Abstract
Biogenic production of nanoparticles is eco-friendly, less expensive method with various medical and biological applications. Nanotechnology along with photodynamic therapy is gaining tremendous importance with enhanced efficacy. The present work was aimed to evaluate methanolic extracts and nanoparticles of two selected plants (Datura suavolens and Verbina tenuisecta) for cytotoxic photodynamic, antioxidant and antimicrobial study. Both extract and silver (5 mM) nanoparticles of Datura plant showed significant activities against bacterial strains. Maximum ZOI of 27.3 ± 1.6 mm was observed with nanoparticles of Datura branches with minimum inhibitory (MIC) value of 32 µg/ml. In case of antifungal and antioxidant assay samples were moderately active. Silver nanoparticles and extracts were effective against rhabdomyosarcoma cell line with lowest IC50 value of 42.5 ± 0.6 μg/ml and percent viability of 25.6 ± 1.3 of Verbena tenuisecta. However, nanoparticles of Datura leaves and branches were more potent with IC50 value of 2.4 ± 0.9 μg/ml and 7.8 ± 1.1 μg/ml respectively. The result of photodynamic study showed that efficacy of photosensitizer was enhanced and percent viability reduced when nanoparticles used as an adjunct. The color change and UV spectra (415‒425 nm) indicated the production of nanoparticles. Fourier transform infrared spectroscopy (FTIR) spectra showed presence of different functional groups e.g., hydroxyl, carbonyl and amino. Nanoparticles are sphenoid in morphology and size ranges between 20–150 nm. Current study showed these silver nanoparticles can be used as cytotoxic agent in photodynamic therapy and can play a critical role to establish medicinal potential of selected plants.
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Abdelshafeek KA, Elgattar AA, Zarkoon AH, Alwahash MA, Shahat AA (2010) Investigation of the volatile oils, lipid constituents and biological activity of Ballota andreuzziana, Teucrium zanonii and Verbena tenuisecta in Libya. Asian Pac J Trop Dis 3(8):594–601
Antony JJ, Sivalingam P, Siva D, Kamalakkannan S, Anbarasu K, Sukirtha R, Krishnan M, Achiraman S (2011) Comparative evaluation of antibacterial activity of silver nanoparticles synthesized using Rhizophora apiculata and glucose. Colloids Surf B Biointerfaces 88(1):134–140
Aromal SA, Philip D (2012) Green synthesis of gold nanoparticles using Trigonella foenum-graecum and its size-dependent catalytic activity. Spectrochim Acta A 97:1–5
Aziz MH, Alam FM, Fatima M, Shaheen F, Iqbal S, Atif M, Talha M, Ali SM, Afzal M, Majid A, Harbi TS (2016) Photodynamic effect of Ni nanotubes on an HeLa cell line. PLoS ONE 11(3):e0150295
Begum F, Haque MR, Nahar KS, Rashid MA (2015) Secondary metabolites from different extractives of Stereospermum suaveolens. Dhaka Univ J Pharm Sci 13(1):31–36
Butler MS (2004) The role of natural product chemistry in drug discovery. J Nat Prod 67(12):2141–2153
Calvo MI (2006) Anti-inflammatory and analgesic activity of the topical preparation of Verbena officinalis L. J Ethnopharmacol 107(3):380–382
Chandran SP, Chaudhary M, Pasricha R, Ahmad A, Sastry M (2006) Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. Biotechnol prog 22(2):577–583
Chen S, Webster S, Czerw R, Xu J, Carroll DL (2004) Morphology effects on the optical properties of silver nanoparticles. J Nanosci Nanotechnol 4(3):254–259
El-Hussein A (2016) Study DNA damage after photodynamic therapy using silver nanoparticles with A549 cell line. J Nanomed Nanotechnol 7(346):2
Fatima N, Kondratyuk TP, Park EJ, Marler LE, Jadoon M, Qazi MA, Mehboob Mirza H, Khan I, Atiq N, Chang LC (2016) Endophytic fungi associated with Taxus fuana (West Himalayan Yew) of Pakistan: potential bio-resources for cancer chemopreventive agents. Pharm biol 54(11):2547–2554
Gajbhiye M, Kesharwani J, Ingle A, Gade A, Rai M (2009) Fungus-mediated synthesis of silver nanoparticles and their activity against pathogenic fungi in combination with fluconazole. Nanomed Nanotechnol 5(4):382–386
Geller F, Murillo R, Steinhauser L, Heinzmann B, Albert K, Merfort I, Laufer S (2014) Four new flavonol glycosides from the leaves of Brugmansia suaveolens. Molecules 19(5):6727–6736
Gong P, Li H, He X, Wang K, Hu J, Tan W, Zhang S, Yang X (2007) Preparation and antibacterial activity of Fe3O4 and Ag nanoparticles. Nanotechnology 18(28):285604
Jeyaraj M, Rajesh M, Arun R, MubarakAli D, Sathishkumar G, Sivanandhan G, Dev GK, Manickavasagam M, Premkumar K, Thajuddin N, Ganapathi A (2013) An investigation on the cytotoxicity and caspase-mediated apoptotic effect of biologically synthesized silver nanoparticles using Podophyllum hexandrum on human cervical carcinoma cells. Colloids Surf B 102:708–717
Kanwal ZG, Hafeez A, Haq IU, Tofeeq-Ur-Rehman MSA, Shazadi I, Fatima N, Rehman NU (2016) Antioxidant, antimicrobial and antileishmanial study of different parts of Peganum harmala. Int J Biosci 9:45–58
Li Y, Satake M, Oshima Y, Ohizumi Y (2003) Naphthoquinone and Iridoid with NGF-potentiating activity from Verbena littoralis. Chem Lett 32(8):728–729
Li Y, Wang J, Zhang X, Guo W, Li F, Yu M, Kong X, Wu W, Hong Z (2015) Highly water-soluble and tumor-targeted photosensitizers for photodynamic therapy. Org Biomol Chem 13(28):7681–7694
Masood F, Chen P, Yasin T, Fatima N, Hasan F, Hameed A (2013) Encapsulation of Ellipticine in poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) based nanoparticles and its in-vitro application. Mater Sci Eng C 33(3):1054–1060
Monira KM, Munan SM (2012) Review on Datura metel: A potential medicinal plant. Glob J Res Med Plants Indig Med 1(4):123
Ogawara KI, Higaki K (2017) Nanoparticle-based photodynamic therapy: current status and future application to improve outcomes of cancer treatment. Chem Pharm Bull 65(7):637–641
Parveen K, Banse V, Ledwani L (2016) Green synthesis of nanoparticles: their advantages and disadvantages. AIP Conf. Proc 1724(1):1–7
Phull AR, Abbas Q, Ali A, Raza H, Zia M, Haq IU (2016) Antioxidant, cytotoxicity and antimicrobial activites of green synthesized silver nanoparticles from crude extract of Bergenia ciliata. Future J Pharm Sci 2(1):31–36
Prabhu D, Arulvasu C, Babu G, Manikandan R, Srinivasan P (2013) Biologically synthesized green silver nanoparticles from leaf extract of Vitex negundo L. induce growth-inhibitory effect on human colon cancer cell line HCT15. Process Biochem 48(2), 317–324.
Ravichandran V, Vasanthi S, Shalini S, Shah SA, Harish R (2016) Green synthesis of silver nanoparticles using Atrocarpus altilis leaf extract and the study of their antimicrobial and antioxidant activity. Mater Lett 180:264–267
Reichelt KV, Hoffmann-Luecke P, Hartmann B, Weber B, Ley JP, Krammer GE, Swanepoel KM, Engel KH (2012) Phytochemical characterization of South African bush tea (Athrixia phylicoides DC.). S Afr J Bot 83:1–8
Renugadevi K, Inbakandan D, Bavanilatha M, Poornima V (2012) Cissus quadrangularis assisted biosynthesis of silver nanoparticles with antimicrobial and anticancer potentials. Int J Pharm Bio Sci 3(3):437–445
Saeed M, Ren W, Wu A (2018) Therapeutic applications of iron oxide based nanoparticles in cancer: basic concepts and recent advances. Biomater sci 6(4):708–725
Sathishkumar M, Sneha K, Won SW, Cho CW, Kim S, Yun YS (2009) Cinnamon zeylanicum bark extract and powder mediated green synthesis of nano-crystalline silver particles and its bactericidal activity. Colloids Surf B 73(2):332–338
Sathyavathi R, Krishna MB, Rao SV, Saritha R, Rao DN (2010) Biosynthesis of silver nanoparticles using Coriandrum sativum leaf extract and their application in nonlinear optics. Adv Sci Lett 3(2):138–143
Shah GM, Jamal ZA, Hussain M (2013) Phytotherapy among the rural women of District Abbotabad. Pak J Bot 45:253–261
Sherwani MA, Tufail S, Khan AA, Owais M (2015) Gold nanoparticle-photosensitizer conjugate based photodynamic inactivation of biofilm producing cells: potential for treatment of C. albicans infection in BALB/c mice. PLoS ONE 10(7):e0131684.
Silva GA (2004) Introduction to nanotechnology and its applications to medicine. Surg Neurol 61(3):216–220
Song JY, Kim BS (2008) Biological synthesis of bimetallic Au/Ag nanoparticles using Persimmon (Diopyros kaki) leaf extract. Korean J Chem Eng 25(4):808–811
Suman TY, Rajasree SR, Kanchana A, Elizabeth SB (2013) Biosynthesis, characterization and cytotoxic effect of plant mediated silver nanoparticles using Morinda citrifolia root extract. Colloids Surf B 106:74–78
Tang R, Habimana-Griffin LM, Lane DD, Egbulefu C, Achilefu S (2017) Nanophotosensitive drugs for light-based cancer therapy: what does the future hold? Nanomedicine 12(10):1101–1105
Zia F, Ghafoor N, Iqbal M, Mehboob S (2016) Green synthesis and characterization of silver nanoparticles using Cydonia oblong seed extract. Appl Nanosci 6(7):1023–1029
Zia M, Gul S, Akhtar J, Ul Haq I, Abbasi BH, Hussain A, Naz S, Chaudhary MF (2017) Green synthesis of silver nanoparticles from grape and tomato juices and evaluation of biological activities. IET Nanobiotechnol 11(2):193–219
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Current work is funded by CIIT Project No. 16–73/CRGP/CIIT/IBD/15/696
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Ashraf, A., Fatima, N., Shahzadi, I. et al. Datura suaveolens and Verbena tenuisecta mediated silver nanoparticles, their photodynamic cytotoxic and antimicrobial evaluation. World J Microbiol Biotechnol 36, 31 (2020). https://doi.org/10.1007/s11274-019-2787-6
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DOI: https://doi.org/10.1007/s11274-019-2787-6