Skip to main content
SpringerLink
Log in

Biosynthesis of 1,3-propanediol from recombinant E. coli by optimization process using pure and crude glycerol as a sole carbon source under two-phase fermentation system

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

The environmental and nutritional condition for 1,3-propanediol (1,3-PD) production by the novel recombinant E. coli BP41Y3 expressing fusion protein were first optimized using conventional approach. The optimum environmental conditions were: initial pH at 8.0, incubation at 37 °C without shaking and agitation. Among ten nutrient variables, fumarate, (NH4)2HPO4 and peptone were selected to study on their interaction effect using the response surface methodology. The optimum medium contained modified Riesenberg medium (containing pure glycerol as a sole carbon source) supplemented with 63.65 mM fumarate, 3.80 g/L (NH4)2HPO4 and 1.12 g/L peptone, giving the maximum 1,3-PD production of 2.43 g/L. This was 3.5-fold higher than the original medium (0.7 g/L). Two-phase cultivation system was conducted and the effect of pH control (at 6.5, 7.0 and 8.0) was investigated under anaerobic condition by comparing with the no pH control condition. The cultivation system without pH control (initial pH of 8.0) gave the maximum values of 1.65 g/L 1,3-PD, the 1,3-PD production rate of 0.13 g/L h and the yield of 0.31 mol 1,3-PD/mol crude glycerol. Hence, using crude glycerol as a sole carbon source resulted in 32 % lower 1,3-PD production from this recombinant strain that may be due to the presence of various impurities in the crude glycerol of biodiesel plant. In addition, succinic acid was found to be a major product during fermentation by giving the maximum concentration of 11.92 g/L after 24 h anaerobic cultivation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Abbad-Andaloussi S, Amine J, Gerard P, Petitdemange H (1998) Effect of glucose on glycerol metabolism by Clostridium butyricum DSM 5431. J Appl Microbiol 84:515–522

    Article  CAS  Google Scholar 

  • Aghaie E, Pazouki M, Hosseimi MR, Ranjbar M, Ghavipanjeh F (2009) Response surface methodology (RSM) analysis of organic acid production for kaolin beneficiation by Aspergillus niger. Chem Eng J 147:245–251

    Article  CAS  Google Scholar 

  • Barbirato F, Camarasa C, Claret Grivet JP, Bories A (1995) Glycerol fermentation by a new 1,3-propanediol producing microorganism: Enterobacter agglomerans. Appl Microbiol Biotechnol 43:786–793

    Article  CAS  Google Scholar 

  • Barbirato F, Grivet JP, Soucaille P, Bories A (1996) 3-Hydroxypropionaldehyde, an inhibitory metabolite of glycerol fermentation to 1,3-propanediol by enterobacterial species. Appl Environ Microbiol 62:1448–1451

    CAS  Google Scholar 

  • Biebl H, Martin S (1995) Fermentation of glycerol to 1,3-propanediol: use of cosubstrates. Appl Microbiol Biotechnol 44:15–19

    Article  CAS  Google Scholar 

  • Cheek J, Broderick JB (2001) Adenosylmethionine-dependent iron-sulfur enzymes: versatile clusters in a radical new role. J Biol Inorg Chem 6:209–226

    Article  CAS  Google Scholar 

  • Circle SJ, Stone L, Boruff CS (1945) Acrolein determination by means of tryptophan a colorimetric micrometric. Ind Eng Chem Res 17:259–262

    CAS  Google Scholar 

  • Frey PA, Magnusson OT (2003) S-Adenosylmethionine: a wolf in sheep’s clothing, or a rich man’s adenosylcobalamin. Chem Rev 103:2129–2148

    Article  CAS  Google Scholar 

  • Garg SK, Jain A (1995) Fermentative production of 2,3-butanediol: a review. Bioresour Technol 51:103–109

    Article  CAS  Google Scholar 

  • Ghadge SV, Raheman H (2006) Process optimization for biodiesel production from mahua (Madhuca indica) oil using response surface methodology. Bioresour Technol 97:379–384

    Article  CAS  Google Scholar 

  • Laffend LA, Nagarajan V (1997) Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism. US Patents 5,686,276

  • Lin R, Liu H, Hao J, Cheng K, Liu D (2005) Enhancement of 1,3-propanediol production by Klebsiella pneumoniae with fumarate addition. Biotechnol Lett 27:1755–1759

    Article  CAS  Google Scholar 

  • Liu HL, Chiou YR (2005) Optimal decolorization efficiency of Reactive Red 239 by UV/TiO2 photocatalytic process coupled with response surface methodology. Chem Eng J 112:173–179

    Article  CAS  Google Scholar 

  • Malaoui H, Marczak R (2001) Influence of glucose on glycerol metabolism by wild-type and mutant strains of Clostridium butyricum E5 grown in chemostat culture. Appl Microbiol Biotechnol 55:226–233

    Article  CAS  Google Scholar 

  • Meinander N, Zacchi G, Hahn-Hägerdal B (1996) A heterologous reductase affects the redox balance of recombinant Saccharomyces cerevisiae. Microbiology 142:165–172

    Article  CAS  Google Scholar 

  • Mongi F, Edward C, William H, Gwang-Hoon G, Almadidy A (2005) Influence of culture parameters on biological hydrogen production by Clostridium saccharoperbutylacetonicum ATCC 27021. World J Microbiol Biotechnol 21:855–862

    Article  Google Scholar 

  • Oh BR, Seo JW, Choi MH, Kim CH (2008) Optimization of culture conditions for 1,3-propanediol production from crude glycerol by Klebsiella pneumoniae using response surface methodology. Biotechnol Bioproc E 13:666–670

    Article  CAS  Google Scholar 

  • Pirt SJ (1975) Principles of microbe and cell cultivation. Wiley, New York

    Google Scholar 

  • Qi X, Sun L, Luo Z, Wu J, Meng X, Tang Y, Wei Y, Huang R (2006) Rational design of glycerol dehydratase: swapping the genes encoding the subunits of glycerol dehydratase to improve enzymatic properties. Chin Sci Bull 51:2977–2985

    Article  CAS  Google Scholar 

  • Rasch M (2002) The influence of temperature, salt and pH on the inhibitory effect of reuterin on Escherichia coli. Int J Food Microbiol 72:225–231

    Article  CAS  Google Scholar 

  • Raynaud C, Sarcabal P, Meynial-Salles I, Croux C, Soucaille P (2003) Molecular characterization of the 1,3-propanediol operon of Clostridium butyricum encoding a novel coenzyme B12 independent glycerol dehydrataseand a 1,3-propanediol dehydrogenase. Proc Natl Acad Sci USA 100:5010–5015

    Article  CAS  Google Scholar 

  • Riesenberg D, Schulz V, Knorre WA, Pohl HD, Korz D, Sanders EA et al (1991) High cell density cultivation of Escherichia coli at controlled specific growth rate. J Biotechnol 20:17–28

    Article  CAS  Google Scholar 

  • Rujananon R, Prasertsan P, Phongdara A, Panrat T, Sun J, Rappert S, Zeng AP (2011) Construction of recombinant E. coli expressing fusion protein to produce 1,3-propanediol. Int J Biol Med Sci 1(1):26–32

    Google Scholar 

  • Sattayasamitsathit S, Prasertsan P, Methacanon P (2011) Statistical optimization for simultaneous production of 1,3-propanediol and 2,3-butanediol using crude glycerol by newly bacterial isolate. Process Biochem 46:608–614

    Article  CAS  Google Scholar 

  • Saxena R, Anand P, Saran S, Isar J (2009) Microbial production of 1,3-propanediol: recent developments and emerging opportunities. Biotechnol Adv 27:895–913

    Article  CAS  Google Scholar 

  • Skraly FA, Lytle BL, Cameron DC (1998) Construction and characterization of a 1,3-propanediol operon. Appl Environ Microbiol 64:98–105

    CAS  Google Scholar 

  • Sun J, Heuvel J, Soucaille P, Qu Y, Zeng AP (2003) Comparative genomic analysis of dha regulon and related genes for anaerobic glycerol metabolism in bacteria. Biotechnol Prog 19:263–272

    Article  CAS  Google Scholar 

  • Tang X, Tan Y, Zhu H, Zhao K, Shen W (2009) Microbial conversion of glycerol to 1,3-propanediol by an engineered strain of Escherichia coli. Appl Environ Microbiol 75:1628–1634

    Article  CAS  Google Scholar 

  • Tong IT, Liao HH, Cameron DC (1991) 1,3-Propanediol production by Escherichia coli expressing genes from the Klebsiella pneumoniae dha regulon. Appl Environ Microbiol 57:3541–3546

    CAS  Google Scholar 

  • Wang F, Qu H, Zhang D, Tian P, Tan T (2007) Production of 1,3-propanediol from glycerol by recombinant E. coli using incompatible plasmids system. Mol Biotechnol 37:112–119

    Article  CAS  Google Scholar 

  • Zeng AP, Biebl H, Schlieker H, Deckwer WD (1993) Pathway analysis of glycerol fermentation by Klebsiella pneumoniae: regulation of reducing equivalent balance and product formation. Enz Microb Technol 15:770–779

    Article  CAS  Google Scholar 

  • Zhang GL, Ma BB, Xu XL, Li C, Wang L (2007) Fast conversion of glycerol to 1,3-propanediol by a new strain of Klebsiella pneumoniae. Biochem Eng J 37:256–260

    Article  CAS  Google Scholar 

  • Zheng ZM, Hu QL, Hao J, Xu F, Guo NN, Sun Y et al (2008) Statistical optimization of culture conditions for 1,3-propanediol by Klebsiella pneumoniae AC15 via central composite design. Bioresour Technol 99:1052–1056

    Article  CAS  Google Scholar 

  • Zhu MM, Lawman PD, Cameron DC (2002) Improving 1,3-propanediol production from glycerol in a metabolically engineered Escherichia coli by reducing accumulation of sn-Glycerol-3-phosphate. Biotechnol Prog 18:694–699

    Article  CAS  Google Scholar 

  • Zhuge B, Zhang C, Fang H, Zhuge J, Permaul K (2010) Expression of 1,3-propanediol oxidoreductase and its isoenzyme in Klebsiella pneumoniae for bioconversion of glycerol into 1,3-propanediol. Appl Microbiol Biot 87:2177–2184

    Article  CAS  Google Scholar 

  • Zinatizadeh AAL, Mohamed AR, Najafpour GD, Isa MH, Nasrollahzadeh H (2006) Kinetic evaluation of high rate POME digestion in an UASFF bioreactor. Process Biochem 41:1038–1046

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank the Royal Golden Jubilee (RGJ) Ph.D Program of Thailand Research fund (TRF), Graduate School and Faculty of Agro-Industry, Prince of Songkla University and the Annual Government Budget, Thailand for the financial support of this work.

Author information

Authors and Affiliations

  1. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Songkhla, 90112, Thailand

    Rosarin Rujananon & Poonsuk Prasertsan

  2. Palm Oil Products and Technology Research Center (POPTEC), Faculty of Agro-Industry, Prince of Songkla University, Songkhla, 90112, Thailand

    Poonsuk Prasertsan

  3. Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Songkhla, 90112, Thailand

    Amornrat Phongdara

Authors
  1. Rosarin Rujananon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Poonsuk Prasertsan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Amornrat Phongdara
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Poonsuk Prasertsan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rujananon, R., Prasertsan, P. & Phongdara, A. Biosynthesis of 1,3-propanediol from recombinant E. coli by optimization process using pure and crude glycerol as a sole carbon source under two-phase fermentation system. World J Microbiol Biotechnol 30, 1359–1368 (2014). https://doi.org/10.1007/s11274-013-1556-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-013-1556-1

Keywords

Search

Navigation