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Cloning and biochemical characterization of a glucosidase from a marine bacterium Aeromonas sp. HC11e-3

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Abstract

By constructing the genomic library, a β-glucosidase gene, with a length of 2,382 bp, encoding 793 amino acids, designated bgla, is cloned from a marine bacterium Aeromonas sp. HC11e-3. The enzyme is expressed successfully in the recombinant host Escherichia coli BL21 (DE3) and purified using glutathione affinity purification system. It shows the optimal activity at pH 6, 55 °C and hydrolyzes aryl-glucoside specially. Ca2+, Mn2+, Zn2+, Ba2+, Pb2+, Sr2+ can activate the enzyme activity, whereas SDS, EDTA, DTT show slight inhibition to the enzyme activity. Homologous comparing shows that the enzyme belongs to glycosyl hydrolase family 3, exhibiting 46 % identity with a fully characterized glucosidase from Thermotoga neapolitana DSM 4359. Such results provide useful references for investigating other glucosidases in the glycosyl family 3 as well as developing glucosidases using in suitable industrial area.

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Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (NO.u1170303) and the open project of state key laboratory of Huazhong Agricultrual University (ALM0810).

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Authors and Affiliations

  1. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Xiaoluo Huang, Yan Zhao, Yunjing Dai, Gaobing Wu & Ziduo liu

  2. Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State of Oceanic Administration, Xiamen, 361005, People’s Republic of China

    Zongze Shao

  3. Department of Biological Engineering, Xianning Vocational Technical College, Xianning, 437100, People’s Republic of China

    Qinglan Zeng

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  1. Xiaoluo Huang
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  2. Yan Zhao
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  3. Yunjing Dai
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  4. Gaobing Wu
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  5. Zongze Shao
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  6. Qinglan Zeng
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  7. Ziduo liu
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Correspondence to Ziduo liu.

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Xiaoluo Huang and Yan Zhao contribute equally to the work.

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Huang, X., Zhao, Y., Dai, Y. et al. Cloning and biochemical characterization of a glucosidase from a marine bacterium Aeromonas sp. HC11e-3. World J Microbiol Biotechnol 28, 3337–3344 (2012). https://doi.org/10.1007/s11274-012-1145-8

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  • DOI: https://doi.org/10.1007/s11274-012-1145-8

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