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Integrated approach for disease management and growth enhancement of Sesamum indicum L. utilizing Azotobacter chroococcum TRA2 and chemical fertilizer

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Abstract

Azotobacter chroococcum TRA2, an isolate of wheat rhizosphere displayed plant growth promoting attributes including indole acetic acid, HCN, siderophore production, solubilization of inorganic phosphate and fixation of atmospheric nitrogen. In addition, it showed strong antagonistic effect against Macrophomina phaseolina and Fusarium oxysporum. It also caused degradation and digestion of cell wall components, resulting in hyphal perforations, empty cell (halo) formation, shrinking and lysis of fungal mycelia along with significant degeneration of conidia. Fertilizer adaptive variant strain of A. chroococcum TRA2 was studied with Tn5 induced streptomycin resistant transconjugants of wild type tetracycline-resistant TRA2 (designated TRA2tetra+strep+) after different durations. The strain was significantly competent in rhizosphere, as its population increased by 15.29 % in rhizosphere of Sesamum indicum. Seed bacterization with the strain TRA2 resulted in significant increase in vegetative growth parameters and yield of sesame over the non-bacterized seeds. However, application of TRA2 with half dose of fertilizers showed sesame yield almost similar to that obtained by full dose treatment. Moreover, the oil yield increased by 24.20 %, while protein yield increased by 35.92 % in treatment receiving half dose of fertilizer along with TRA2 bacterized seeds, as compared to untreated control.

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Acknowledgments

The authors wish to thank the TMOP & M, CSIR (New Delhi), UGC (New Delhi), UCOST (Dehradun) and CST (UP) India for financial assistance.

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Authors and Affiliations

  1. Department of Botany and Microbiology, Gurukul Kangri University, Haridwar, 249404, Uttar Pradesh, India

    D. K. Maheshwari, R. C. Dubey, Abhinav Aeron, Bhavesh Kumar & Sandeep Kumar

  2. Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University, Lucknow, 226025, Uttar Pradesh, India

    Sakshi Tewari & Naveen Kumar Arora

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  1. D. K. Maheshwari
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  2. R. C. Dubey
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  3. Abhinav Aeron
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  4. Bhavesh Kumar
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  5. Sandeep Kumar
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  7. Naveen Kumar Arora
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Correspondence to Naveen Kumar Arora.

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Maheshwari, D.K., Dubey, R.C., Aeron, A. et al. Integrated approach for disease management and growth enhancement of Sesamum indicum L. utilizing Azotobacter chroococcum TRA2 and chemical fertilizer. World J Microbiol Biotechnol 28, 3015–3024 (2012). https://doi.org/10.1007/s11274-012-1112-4

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  • DOI: https://doi.org/10.1007/s11274-012-1112-4

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