Abstract
The present study aimed to isolate actinobacteria from soil samples and characterized them using molecular tools and screened their secondary metabolites for antimicrobial activities. Thirty-nine strains from four different location of Barrientos Island, Antarctica using 12 types of isolation media was isolated. The isolates were preceded to screening of secondary metabolites for antimicrobial and antifungal activities. Using high-throughput screening methods, 38% (15/39) of isolates produced bioactive metabolites. Approximately 18% (7/39), 18% (7/39), 10% (4/39) and 2.5% (1/39) of isolates inhibited growth of Candida albicans ATCC 10231T, Staphylococcus aurues ATCC 51650T, methicillin-resistant Staphylococcus aurues (MRSA) ATCC BAA-44T and Pseudomonas aeruginosa ATCC 10145T, respectively. Molecular characterization techniques like 16S rRNA analysis, Enterobacterial repetitive intergenic consensus—polymerase chain reaction (ERIC-PCR), Random amplified polymorphic DNA (RAPD) and composite analyses were used to characterize the actinobacteria strains. Analysis of 16S rRNA sequences is still one of the most powerful methods to determine higher taxonomic relationships of Actinobacteria. Both RAPD and ERIC-PCR fingerprinting have shown good discriminatory capability but RAPD proved to be better in discriminatory power than ERIC-PCR. Our results demonstrated that composite analysis of both fingerprinting generally increased the discrimination ability and generated best clustering for actinobacteria strains in this study.
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Acknowledgments
The authors are grateful to Instituto Antártico Ecuatoriano (INAE) for their support during the XI Ecuadorian Antarctic Expedition to “Pedro Vicente Maldonado” Research Station, Greenwich Island, South Shetlands Islands. The authors are thankful to Gao Zhu-Fen and Li Xiao-Hui for laboratory assistance. This work was supported under the Malaysia Antarctic Research Program, Academy of Sciences Malaysia and Universiti Putra Malaysia Research Universiti Grant Scheme (05-01-11-1219RU).
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Lee, LH., Cheah, YK., Mohd Sidik, S. et al. Molecular characterization of Antarctic actinobacteria and screening for antimicrobial metabolite production. World J Microbiol Biotechnol 28, 2125–2137 (2012). https://doi.org/10.1007/s11274-012-1018-1
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DOI: https://doi.org/10.1007/s11274-012-1018-1