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Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1

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Abstract

A locally isolated Acinetobacter sp. Strain AQ5NOL 1 was encapsulated in gellan gum and its ability to degrade phenol was compared with the free cells. Optimal phenol degradation was achieved at gellan gum concentration of 0.75% (w/v), bead size of 3 mm diameter (estimated surface area of 28.26 mm2) and bead number of 300 per 100 ml medium. At phenol concentration of 100 mg l−1, both free and immobilized bacteria exhibited similar rates of phenol degradation but at higher phenol concentrations, the immobilized bacteria exhibited a higher rate of degradation of phenol. The immobilized cells completely degrade phenol within 108, 216 and 240 h at 1,100, 1,500 and 1,900 mg l−1 phenol, respectively, whereas free cells took 240 h to completely degrade phenol at 1,100 mg l−1. However, the free cells were unable to completely degrade phenol at higher concentrations. Overall, the rates of phenol degradation by both immobilized and free bacteria decreased gradually as the phenol concentration was increased. The immobilized cells showed no loss in phenol degrading activity after being used repeatedly for 45 cycles of 18 h cycle. However, phenol degrading activity of the immobilized bacteria experienced 10 and 38% losses after the 46 and 47th cycles, respectively. The study has shown an increased efficiency of phenol degradation when the cells are encapsulated in gellan gum.

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Acknowledgments

This work was supported by Research Grant Scheme (RUGS) 2009, Universiti Putra Malaysia (91851).

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang, 43400, Selangor, Malaysia

    Siti Aqlima Ahmad, Noorliza Mat Arif, Gan Bee Koon, Mohd Yunus Abdul Shukor & Mohd Arif Syed

  2. Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, 13th Floor, Menara B, Persiaran MPAJ, Jalan Pandan Utama, Pandan Indah, 55100, Kuala Lumpur, Malaysia

    Nor Aripin Shamaan

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  1. Siti Aqlima Ahmad
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  2. Nor Aripin Shamaan
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  3. Noorliza Mat Arif
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  5. Mohd Yunus Abdul Shukor
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  6. Mohd Arif Syed
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Correspondence to Mohd Arif Syed.

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Ahmad, S.A., Shamaan, N.A., Arif, N.M. et al. Enhanced phenol degradation by immobilized Acinetobacter sp. strain AQ5NOL 1. World J Microbiol Biotechnol 28, 347–352 (2012). https://doi.org/10.1007/s11274-011-0826-z

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  • DOI: https://doi.org/10.1007/s11274-011-0826-z

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