Abstract
Berkleasmium sp. Dzf12, an endophytic fungus from Dioscorea zingiberensis, is a high producer of the spirobisnaphthalene diepoxin ζ. However, diepoxin ζ produced by Berkleasmium sp. Dzf12 was retained as both the intracellular and extracellular product. This study was to evaluate an in situ resin adsorption for enhancement of diepoxin ζ production in mycelial liquid culture of Berkleasmium sp. Dzf12. Diepoxin ζ production was most effectively enhanced by macroporous resin AB-8 among five test resins. The highest diepoxin ζ yield reached 448.6 mg l−1 that was 1.4 fold of the control (329.7 mg l−1), when 1.5 g of resin AB-8 was added to 30 ml medium in each flask on day 11 of culture and in a period of 40 h for adsorption. The results show that in situ resin adsorption is an effective strategy for enhancing diepoxin ζ production and also facilitating its recovery in mycelial liquid culture of Berkleasmium sp. Dzf12.
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Acknowledgments
This work was co-financed by the grants from the National Natural Science Foundation of China (31071710 and 30871662), the Hi-Tech R&D Program of China (2011AA10A202), and the Natural Science Foundation of Beijing (6092015).
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Zhao, J., Li, Y., Shan, T. et al. Enhancement of diepoxin ζ production with in situ resin adsorption in mycelial liquid culture of the endophytic fungus Berkleasmium sp. Dzf12 from Dioscorea zingiberensis . World J Microbiol Biotechnol 27, 2753–2758 (2011). https://doi.org/10.1007/s11274-011-0750-2
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DOI: https://doi.org/10.1007/s11274-011-0750-2