Skip to main content
SpringerLink
Log in

Comparison of different methods for release of Bifidobacterium longum Bb46 from the poly(vinylpyrrolidone)-poly(vinylacetate-co-crotonic acid) interpolymer complex matrix, and the effect of grinding on the microparticles

  • Short Communication
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

Bifidobacteria have been efficiently encapsulated in poly(vinylpyrrolidone)-poly(vinylacetate-co-crotonic acid) (PVP: PVAc-CA) interpolymer complex formed in scCO2. Research indicated that this method improves the stability of encapsulated bacteria in simulated gastrointestinal fluids in vitro. However, further analysis indicated release of lower numbers of encapsulated bacteria from the encapsulating matrix. The aims of this study were to determine a method that would release high numbers of bacteria from the PVP: PVAc-CA interpolymer complex matrix microparticles, and furthermore, to determine the effects of milling on the morphological properties of the microparticles. Three release methods, namely sonication, homogenization in a stomacher and incubation in simulated intestinal fluid were compared. Released viable bacteria were assayed using plate counts. Viable bacteria released using a stomacher were three orders of magnitude higher than those released by incubation and an order of magnitude higher than those released using sonication. SEM indicated no negative effects such as exposure of encapsulated bacteria on the matrix due to milling of product. Homogenization in a stomacher is the most efficient method for releasing bacteria from the PVP: PVAc-CA interpolymer complex matrix. Particle size of the PVP: PVAc-CA microparticles encapsulating bacteria can be reduced further by grinding, without exposing the enclosed bacteria.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

References

  • Annan NT, Borza AD, Truelstrup Hansen L (2008) Encapsulation in alginate-coated gelatin microspheres improves survival of the probiotic Bifidobacterium adolescentis 15703T during exposure to simulated gastro-intestinal conditions. Food Res Int 41:184–193

    Article  CAS  Google Scholar 

  • Blum JS, Saltzman WM (2008) High loading efficiency and tunable release of plasmid DNA encapsulated in submicron particles fabricated from PLGA conjugated with poly-l-lysine. J Contr Release 129:66–72

    Article  CAS  Google Scholar 

  • Champagne CP, Yves R, Tompkins TA (2010) The determination of viable counts in probiotic cultures microencapsulated by spray-coating. Food Microbiol. doi:10.1016/j.fm.2010.07.017

  • Chandramouli V, Kailasapathy K, Peiris P, Jones M (2004) An improved method of microencapsulation and its evaluation to protect Lactobacillus spp. in simulated gastric conditions. J Microbiol Meth 56:27–36

    Article  CAS  Google Scholar 

  • Doleyres Y, Lacroix C (2005) Technologies with free and immobilized cells for probiotic bifidobacteria production and protection. Int Dairy J 15:973–988

    Article  CAS  Google Scholar 

  • Godward G, Kailasapathy K (2003) Viability and survival of free, encapsulated and co-encapsulated probiotic bacteria in yoghurt. Milchwissenschaft 58:396–399

    Google Scholar 

  • Homayouni A, Azizi A, Ehsani MR, Yarmand MS, Razavi SH (2008) Effect of microencapsulation and resistant starch on the probiotic survival and sensory properties of synbiotic ice cream. Food Chem 111:50–55

    Article  CAS  Google Scholar 

  • Krasaekoopt W, Bhandar B, Deeth H (2003) Evaluation of encapsulation techniques of probiotics for yoghurt: a review. Int Dairy J 13:3–13

    Article  CAS  Google Scholar 

  • Lo P-R, Chou C-C, Yu R-C, Tsai Y-H (2002) Antimutagenic activity of several probiotic bifidobacteria against benzo[a]pyrene. J Biosci Bioeng 94:148–153

    Article  CAS  Google Scholar 

  • Lyer C, Kailasapathy K, Peiris P (2004) Evaluation of survival and release of encapsulated bacteria in ex vivo porcine gastrointestinal contents using a green fluorescent protein gene-labelled E. coli. Food Sci Technol 37:639–642

    Google Scholar 

  • Ma Y, Huang X, Korenevsky A, Pacan JC, Sabour PM, Wang Q, Xu Y (2008) Microencapsulation of bacteriophage felix O1 into chitosan-alginate microspheres for oral delivery. Appl Environ Microb 74:4799–4805

    Article  CAS  Google Scholar 

  • Madigan MT, Martinko JM (2006) Brock biology of microorganisms. Pearson Prentice Hall, USA

    Google Scholar 

  • Moolman FS, Labuschagne PW, Rolfes H, Thantsha MS, van der Merwe TL, Cloete TE (2006) Encapsulating probiotics with an interpolymer complex in supercritical carbon dioxide. S Afr J Sci 102:349–354

    CAS  Google Scholar 

  • Nguyen TMP, Lee KY, Zhou W (2009) Stimulating fermentative activities of bifidobacteria in milk by high intensity ultrasound. Int Dairy J 19:410–416

    Article  CAS  Google Scholar 

  • Picot A, Lacroix C (2003) Effects of micronization on viability and thermotolerance of probiotic freeze-dried cultures. Int Dairy J 13:455–462

    Article  Google Scholar 

  • Picot A, Lacroix C (2004) Encapsulation of bifidobacteria in whey protein-based microcapsules and survival in simulated gastrointestinal conditions and in yoghurt. Int Dairy J 14:505–515

    Article  CAS  Google Scholar 

  • Sensoy D, Cevher E, Özdamar A, Sarici A, Yilmaz M, Bergişadi N (2009) Bioadhesive sulfacetamide sodium microspheres: evaluation of their effectiveness in the treatment of bacterial keratitis caused by Staphylococcus aureus and Pseudomonas aeruginosa in a rabbit model. Eur J Pharm Biopharm 72:487–495

    Article  CAS  Google Scholar 

  • Sheu TY, Marshall RT (1993) Micro-encapsulation of Lactobacilli in calcium alginate gels. J Food Sci 54:557–561

    Article  Google Scholar 

  • Sultana K, Arumugaswamy R, Godward G, Peiris P, Reynolds N, Kailasapathy K (2000) Encapsulation of probiotic bacteria with alginate–starch and evaluation of survival in simulated gastrointestinal conditions and in yoghurt. Int J Food Microbiol 62:47–55

    Article  CAS  Google Scholar 

  • Thantsha MS (2007) Cell immobilization techniques for the preservation of probiotics. Dissertation, University of Pretoria

  • Thantsha MS, Cloete TE, Moolman FS, Labuschagne PW (2009) Supercritical carbon dioxide interpolymer complexes improve survival of B. longum Bb-46 in simulated gastrointestinal fluids. Int J Food Microbiol 129:88–92

    Article  CAS  Google Scholar 

  • United States Pharmacopeial Convention (2005) United States pharmacopeia, 25th edn. Webcom, Toronto

    Google Scholar 

  • Xiaoyan LI, Xiguang C (2009) Drying of micro-encapsulated lactic acid bacteria effects of trehalose and immobilization on cell survival and release properties. J Ocean Univ China 8:39–44

    Article  Google Scholar 

  • Yang X, Chena H, Gaoa F, Wang Y, Zhang H, Zhang O, Liu L (2008) Self-aggregated nanoparticles from methoxy poly(ethylene glycol)-modified chitosan: synthesis; characterization; aggregation and methotrexate release in vitro. Colloid Surface B 61:125–131

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank the National Research Foundation of South Africa for funding of the project, the Laboratory for Microscopy and Microanalysis of the University of Pretoria for assistance with the SEM work and Professor Teresa Coutinho for language editing.

Author information

Authors and Affiliations

  1. Department of Microbiology and Plant Pathology, New Agricultural Building, University of Pretoria, Pretoria, 0002, South Africa

    M. S. Thantsha & J. Guest

  2. Polymers, Ceramics and Composites, Council For Scientific and Industrial Research (CSIR), Pretoria, South Africa

    I. Mputle

Authors
  1. M. S. Thantsha
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. Guest
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. Mputle
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. S. Thantsha.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Thantsha, M.S., Guest, J. & Mputle, I. Comparison of different methods for release of Bifidobacterium longum Bb46 from the poly(vinylpyrrolidone)-poly(vinylacetate-co-crotonic acid) interpolymer complex matrix, and the effect of grinding on the microparticles. World J Microbiol Biotechnol 27, 2443–2448 (2011). https://doi.org/10.1007/s11274-011-0691-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11274-011-0691-9

Keywords

Search

Navigation