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Identification of DC-SIGN as the receptor during the interaction of Lactobacillus plantarum CGMCC 1258 and dendritic cells

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Abstract

Lactobacillus plantarum can exert additional probiotic effects via regulation of human immune system. However, the direct interaction between probiotics and the receptors of immune cells still needs to be further studied. To identify the receptor of dendritic cells during the interaction with L. plantarum. Dendritic cells were pretreated with L. plantarum and the antibody to dendritic cells specific intercellular adhesion molecule-grabbing nonintegrin (DC-SIGN), toll like receptor (TLR)-2 and TLR-4. The maturation of immature dendritic cells, cytokine production, and modulation of T cells were studied by flow cytometry. Adherence between L. plantarum and dendritic cells were studied by ELISA, flow cytometry, and Western blot. L. plantarum could mature dendritic cells by up-regulating MHC-II and CD80 and CD86. Anti-inflammatory interlectin (IL)-10 and IL-6 was up-regulated and pro-inflammatory IL-12p70 was retro-regulated by L. plantarum. L. plantarum may interact with DC-SIGN and modulate of T to differentiate into IL-4 producing T cells. The interaction of L. plantarum and DC-SIGN and the biological effects could be blocked by EDTA and antibody to DC-SIGN. Effects of L. plantarum were concentration-dependent. L. plantarum could bind to DC-SIGN to improve DC maturation at different ratios, regulate the secretion of anti-inflammatory and pro-inflammatory cytokines, and induce the polarization of interlectin-4-producing T cells.

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Acknowledgments

The authors thank Dr. Hang Xiaomin (Institute of Science Life of Only, Shanghai Jiao Tong University, Shanghai, China) for generous provision of L. plantarum CGMCC 1258. The authors thank Shanghai Jiao Tong University Affiliated Sixth People’s Hospital for technical assistance during this study. This work was financially supported by National Natural Science Foundation of China (No. 30672044), and the National Basic Research Program of China (No. 2008CB517403).

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Authors and Affiliations

  1. Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 600 Yishan Road, Shanghai, 200233, China

    Zhihua Liu, Yanlei Ma, Hongqi Chen, Yukun Zhou, Peng Zhang, Ming Zhang, Zhaoxin Chu & Huanlong Qin

  2. Department of Surgery, Shanghai Tenth People’s Hospital, Shanghai, China

    Tongyi Shen

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  1. Zhihua Liu
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Correspondence to Huanlong Qin.

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Liu, Z., Ma, Y., Shen, T. et al. Identification of DC-SIGN as the receptor during the interaction of Lactobacillus plantarum CGMCC 1258 and dendritic cells. World J Microbiol Biotechnol 27, 603–611 (2011). https://doi.org/10.1007/s11274-010-0495-3

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