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Dye decolorisation by laccase entrapped in copper alginate

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Abstract

A novel immobilisation system was developed for dye decolorisation using laccase produced by Ganoderma sp. KU-Alk4. The enzyme showed high efficiency in dye decolorisation when entrapped in Cu–Al and Cu-alginate beads. The former gave the highest activity but the enzyme activity survived longer in the latter. An experimental design of two 3 × 3 Latin Square experiments was applied to evaluate the effects of three different alginate compositions (low, intermediate and high mannuronate), concentration of alginate, (1.5, 3.0 and 4.5% w/v) and concentration of cross-linking agent, CuSO4 (0.075, 0.15 and 0.225 M) on the decolorisation of indigo carmine dye and residual laccase activity in beads. The most significant factor for residual activity was the concentration of the cross-linking agent (P < 0.05) followed by alginate composition (P < 0.1). Increasing the alginate concentration resulted in only small increase in the dye decolorisation. However, higher laccase activity remained in 3.0% w/v alginate beads. Maximal dye decolorisation was achieved when 3.6% w/v low mannuronate alginate and 0.15 M CuSO4 was used. Optimal conditions were confirmed in an extended experimental run. Results are presented from 9 successive batch runs over 12 days, reaching 96% removal of the dye (216 mg/l).

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Acknowledgements

Grateful to The Royal Golden Jubilee Ph.D. Program of the Thailand Research Fund for support to CT. Thank to Department of Microbiology, Faculty of Science, Kasetsart University.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand

    Churapa Teerapatsakul & Lerluck Chitradon

  2. Emeritus Professor of Biotechnology, School of Biosciences, University of Westminster, 115 New Cavendish Street, London, W1W 6UW, UK

    Christopher Bucke

  3. Department of Applied and Molecular Biosciences, University of Westminster, 115 New Cavendish Street, London, W1W 6UW, UK

    Roberto Parra & Tajalli Keshavarz

Authors
  1. Churapa Teerapatsakul
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  2. Christopher Bucke
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  3. Roberto Parra
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  4. Tajalli Keshavarz
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  5. Lerluck Chitradon
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Correspondence to Lerluck Chitradon.

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Teerapatsakul, C., Bucke, C., Parra, R. et al. Dye decolorisation by laccase entrapped in copper alginate. World J Microbiol Biotechnol 24, 1367–1374 (2008). https://doi.org/10.1007/s11274-007-9617-y

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  • DOI: https://doi.org/10.1007/s11274-007-9617-y

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