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Production of an alkaline protease by Bacillus cereus MCM B-326 and its application as a dehairing agent

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Abstract

The present investigation describes microbial production of an alkaline protease and its use in dehairing of buffalo hide. Bacillus cereus produced extracellular protease when grown on a medium containing starch, wheat bran and soya flour (SWS). The ammonium sulphate precipitated (ASP) enzyme was applied for dehairing of buffalo hide. Microscopic observation of longitudinal section of buffalo hide revealed that the epidermis was completely removed and hair was uprooted leaving empty follicles in the hide. The ASP enzyme was stable for one month at ambient temperature between 25–35 °C. Enzymatic dehairing may be a promising shift towards an environment-friendly leather processing method.

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References

  • Alagarsamy S, Chandran S, George S, Carlos RS, Ashok P (2005) Production and partial purification of a neutral metalloprotease by fungal mixed substrate fermentation. Food Technol Biotechnol 43:313–319

    Google Scholar 

  • Alessandro R, Silvia O, Adriano B (2003) Dehairing activity of extracellular proteases produced by keratinolytic bacteria. J Chem Technol Biotechnol 78:855–859

    Article  Google Scholar 

  • Alexandre JM, Walter O, Beys DS, Renata G, David D, Joao A, Pegas H, Carlos T (2005) Novel Keratinase from Bacillus subtilis S14 exhibiting remarkable dehairing capabilities. Appl Environ Microbiol 71:594–596

    Article  Google Scholar 

  • Amare G (1997) The use of nug meal as a low-cost substrate for the production of alkaline protease by the alkaliphilic Bacillus sp. AR-009 and some properties of the enzyme. Biores Technol 62:59–61

    Article  Google Scholar 

  • Chandrasekaran S, Dhar SC (1985) Studies on the development of a multiple proteinase concentrate and its application in the depilation of skin. Leath Sci 32:297–304

    CAS  Google Scholar 

  • Dayanandan A, Kanagaraj J, Lesley S, Govindaraju R, Rajkumar GS (2003) Application of an alkaline protease in leather processing: an ecofriendly approach. J Clean Product 11:533–536

    Article  Google Scholar 

  • George S, Raju V, Krishnan MRV, Subramanian TV, Jayaraman K (1995) Production of protease by Bacillus amyloliquefaciens in solid state fermentation and its application in the unhairing of hides and skins. Proc Biochem 30:457–462

    Article  CAS  Google Scholar 

  • Ghorbel FB, Sellami KA, Fakhfakh N, Haddar A, Manni L, Nasri M (2005) Production and purification of a calcium-dependent protease from Bacillus cereus BG1. J Ind Microbiol Biotechnol 32:186–194

    Article  Google Scholar 

  • Jaquess PA, Elmore ME, Miguel NB (1999) Advances in stabilized enzymes for leather processing. J Am Leath Chem Asso 94:355–361

    CAS  Google Scholar 

  • Jayaraman J (2003) Biuret method of protein estimation and Lowry’s method. In: Laboratory manual in biochemistry, New Age international (P) ltd., New Delhi. pp 78–79

  • John DB, Merriline J (2002) Theory and practice of histopathalogical techniques. 5th edn. Churchill publishing company

  • Johnvesly B, Manjunath BR, Naik GR (2002) Pigeon pea waste as novel inexpensive, substrate for production of a thermostable alkaline protease from thermoalkalophilic Bacillus sp. JB-99. Biores Technol 82:61–64

    Article  CAS  Google Scholar 

  • Kanagaraj J, Velappan KC, Chandra Babu NK, Sadulla S (2006) Solid waste generation in the leather industry and its utilization for cleaner environment- a review. J Sci Ind Res 65:541–548

    CAS  Google Scholar 

  • Kanekar PP, Nilegaonkar SS, Sarnaik, SS, Kelkar AS (1997) Process for production of protease using deoiled soybean cake and alkaliphilic bacteria Arthobacter ramosus and Bacillus alkalophilus. Indian Patent No.188072

  • Kim SS, Kim YJ, Rhee IK (2001) Purification and characterization of a novel extracellular protease from Bacillus cereus KCTC 3674. Arch Microbiol 175:458–461

    Article  CAS  Google Scholar 

  • Malathi S, Chakraborty R (1991) Production of alkaline protease by a new Aspergillus flavus isolate under solid substrate fermentation conditions for use as a depilation agent. Appl Environ Microbiol 57:712–716

    CAS  Google Scholar 

  • Mukhopadhyay RP, Chandra A (1993) Protease of a keratinolytic streptomycete to unhair goat skin. Ind JExpt Biol 1:557–558

    Google Scholar 

  • Nashy EHA, Ismail SA, Ahmady AM, Fadaly HE, Sayed NH (2005) Enzymatic bacterial dehairing of bovine hide by a locally isolated strain of Bacillus licheniformis. J Soc Leath Technol Chem 89:242–249

    CAS  Google Scholar 

  • Nilegaonkar SS, Kanekar PP, Sarnaik SS, Kelkar AS (2000) Production, isolation and characterization of extracellular protease of an alkaliphilic protease of an alkaliphilic strain of Arthrobacter ramosus, MCM B-351 isolated from the alkaline lake of Lonar, India. World J Microbiol Biotechnol 18:785–789

    Article  Google Scholar 

  • Nilegaonkar SS, Zambare VP, Kanekar PP, Dhakephalkar PK, Sarnaik SS (2007) Production and partial characterization of dehairing protease from Bacillus cereus MCM B-326. Biores Technol 98:1238–1245

    Article  CAS  Google Scholar 

  • Pal S, Banerjee R, Bhattacharyya BC (1996) Application of a proteolytic enzyme in tanneries as a depilating agent. J Am Leather Chem As 91:59–63

    CAS  Google Scholar 

  • Palanisamy T, Jonnlagadda RR, Balachandran UN, Thirumalachari T (2004) Progress and recent trends in biotechnological methods for leather processing. Trend Biotechnol 22:181–188

    Article  Google Scholar 

  • Philippe B, Franccois L, Maria U, Bernard V (1999) Purification and Characterization of a Keratinolytic Serine Proteinase from Streptomyces albidoflavus. Appl Environ Microbiol 65:2570–2576

    Google Scholar 

  • Prakasham RS, Rao CS, Sharma PN (2006) Green gram husk—an inexpensive substrate for alkaline protease production by Bacillus sp. in solid-state fermentation. Biores Technol 97:1449–1454

    Article  CAS  Google Scholar 

  • Purushotham H, Malathi S, Rao PV, Rai CL, Immanuel MM, Raghavan KV (1996) Unhairing enzyme by solid-state fermentation. J Soc Leath Technol Chem 80:52–56

    CAS  Google Scholar 

  • Qing H, Yong P, Xin L, Haifeng W, Yizheng Z (2003) Purification and characterization of an extracellular alkaline serine protease with dehairing function from Bacillus pumilus. Curr Microbiol 46:169–173

    Article  Google Scholar 

  • Roberta CST, Samanta OG, Florencia CO, Adriano B (2006) Optimization of protease production by Microbacterium sp. in feather meal using response surface methodology. Proc Biochem 41:67–73

    Article  Google Scholar 

  • Sinha N, Satyanarayana T (1991) Alkaline protease production by thermophilic Bacillus licheniformis. Ind J Microbiol 31:425–430

    Google Scholar 

  • Thanikaivelan P, Raghava Rao J, Nair U, Ramasami T (2003) Approach towards zero discharge tanning: role of concentration on the development of eco-friendly liming-reliming processes. J Clean Product 11:79–90

    Article  Google Scholar 

  • Varela H, Ferrai MD, Belobrajdic L, Vazquez A, Loperena ML (1997) Skin unhairing proteases of Bacillus subtilis: production and partial characterization. Biotechnol Lett 19:755–758

    Article  CAS  Google Scholar 

  • Yang JK, Shih IL, Tzeng YM, Wang SL (2000) Production and purification of protease from a Bacillus subtilis that can deproteinize crustacean waste. Enz Microb Technol 26:406–411

    Article  CAS  Google Scholar 

  • Yang SS, Wang JY (1999) Protease and amylase production of Streptomyces rimosus in submerged and solid state cultivations. Bot Bull Acad Sin 40:259–265

    CAS  Google Scholar 

Download references

Acknowledgement

The work was carried out under NMITLI project on “Biotechnology for leather-towards cleaner processing”, sponsored by CSIR, Govt. of India.

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Correspondence to P. P. Kanekar.

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Zambare, V.P., Nilegaonkar, S.S. & Kanekar, P.P. Production of an alkaline protease by Bacillus cereus MCM B-326 and its application as a dehairing agent. World J Microbiol Biotechnol 23, 1569–1574 (2007). https://doi.org/10.1007/s11274-007-9402-y

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  • DOI: https://doi.org/10.1007/s11274-007-9402-y

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