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Biodegradation of Endosulfan by Bacterial Strain Alcaligenes faecalis JBW4 in Argi-Udic Ferrosols and Hapli-Udic Isohumosols

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Abstract

The overapplication of endosulfan on crops has resulted in the widespread contamination of soil. In this study, we examine the potential for bioremediation of the bacteria strain Alcaligenes faecalis JBW4 in degrading endsosulfan in soils. Bacteria were inoculated into sterilized and non-sterilized soils (Argi-Udic Ferrosols and Hapli-Udic Isohumosols) spiked with endosulfan. The results obtained from polymerase chain reaction-denaturing gradient gel electrophoresis indicate that JBW4 colonized Argi-Udic Ferrosols and Hapli-Udic Isohumosols successfully. The degradation efficiencies of α and β isomers of endosulfan by JBW4 were higher in Hapli-Udic Isohumosols than in Argi-Udic Ferrosols, and α and β isomers were degraded by 100.0 and 69.8%, respectively. In addition, detected endosulfan metabolites were either endosulfan ether and endosulfan lactone. Results of the single-cell gel electrophoresis assay showed that the toxicity of endosulfan and its metabolites in Hapli-Udic Isohumosols decreased after 77 days when compared to those in Argi-Udic Ferrosols after degradation by JBW4. Strain JBW4 is an excellent bio-remediator through its ability to degrade endosulfan in contaminated Argi-Udic Ferrosols and Hapli-Udic Isohumosols.

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Acknowledgments

We thank the professor of soil and water science, University of Florida, and the student of agricultural science, North Carolina State University for reviewing the manuscript. This study was funded by grants from the National Key Research and Development Plan (No. 2016YFD0800202) and the National Natural Science Foundation of China (No. 21377075).

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Authors and Affiliations

  1. Key Laboratory of Agricultural Environment in the University of Shandong, College of Resources and Environment, Shandong Agricultural University, 61 Daizong Road, Taian, 271018, People’s Republic of China

    Yu Zhang, Lusheng Zhu, Jinhua Wang, Jun Wang, Cheng Zhang, Yuting Shao & Chao Li

  2. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, People’s Republic of China

    Benying Su

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  1. Yu Zhang
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  2. Lusheng Zhu
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  3. Jinhua Wang
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  4. Jun Wang
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  5. Benying Su
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  6. Cheng Zhang
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  7. Yuting Shao
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Correspondence to Lusheng Zhu or Benying Su.

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Zhang, Y., Zhu, L., Wang, J. et al. Biodegradation of Endosulfan by Bacterial Strain Alcaligenes faecalis JBW4 in Argi-Udic Ferrosols and Hapli-Udic Isohumosols. Water Air Soil Pollut 227, 425 (2016). https://doi.org/10.1007/s11270-016-3125-3

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