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Responses of Limagne “Clay/Organic Matter-Rich” Soil Microbial Communities to Realistic Formulated Herbicide Mixtures, Including S-Metolachlor, Mesotrione, and Nicosulfuron

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Abstract

Soil is a primary resource used by mankind to ensure its needs mainly through agriculture. Its sustainability is regulated by the indigenous organisms it contains such as microorganisms. Current agricultural practices employ mixtures of pesticides to ensure the crops yield and can potentially impair these non-target organisms. However despite this environmental reality, studies dealing the susceptibility of microorganisms to pesticide mixtures are scarce. In this context, we designed a 3-month microcosm study to assess the ecotoxicity of realistic herbicide mixtures of formulated S-metolachlor (Dual Gold Safeneur®), mesotrione (Callisto®), and nicosulfuron (Milagro®) on the abundance, the diversity, and the activities of microorganisms from a “clay/organic matter-rich” soil, with a particular attention given to N-cycle communities. These communities appeared to be quite resistant to realistic mixtures even if transient effects occurred on the N-cycle-related communities with an increase of ammonification and an inhibition of nitrification as a short-term effect, followed by an increase of denitrification and an accumulation of nitrates. As nitrates are known to be highly leachable with a strong pollution potential, intensive studies should be carried out at field level to conclude on this potential accumulation and its consequences. Moreover, these data now need to be compared with other agricultural soils receiving these herbicide mixtures in order to bring general conclusion on such practices.

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Acknowledgments

The work of P. Joly was supported by a MENRT doctoral fellowship from the French Ministère de l’Education, de la Recherche et de la Technologie. The authors would like to thank I. Batisson for her wise advices on the numerous fingerprinting tools and the INRA Genosol Plateforme for their technical assistance with the ARISA procedure.

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Authors and Affiliations

  1. INRA, UMR 1095 Génétique, Diversité et Ecophysiologie des Céréales, 5 chemin de Beaulieu, 63039, Clermont-Ferrand, France

    Pierre Joly

  2. Clermont Université, Université Blaise Pascal, LMGE, 63000, Clermont-Ferrand, France

    Frédérique Bonnemoy, Fanny Perrière & Clarisse Mallet

  3. CNRS, UMR 6023, Laboratoire Microorganismes, Génome et Environnement, 63177, Aubière, France

    Frédérique Bonnemoy, Fanny Perrière & Clarisse Mallet

  4. Clermont Université, Université Blaise Pascal, ICCF, BP10448, 63000, Clermont-Ferrand, France

    Pascale Besse-Hoggan

  5. CNRS, UMR 6296, Institut de Chimie de Clermont-Ferrand, 63171, Aubière, France

    Pascale Besse-Hoggan

  6. INRA, UR-251 Pessac, 78026, Versailles Cedex, France

    Olivier Crouzet & Nathalie Cheviron

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  1. Pierre Joly
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Joly, P., Bonnemoy, F., Besse-Hoggan, P. et al. Responses of Limagne “Clay/Organic Matter-Rich” Soil Microbial Communities to Realistic Formulated Herbicide Mixtures, Including S-Metolachlor, Mesotrione, and Nicosulfuron. Water Air Soil Pollut 226, 413 (2015). https://doi.org/10.1007/s11270-015-2683-0

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