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Molecular characterization of picobirnaviruses in small ruminants with diarrhea in Turkey

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Abstract

Picobirnaviruses (PBVs), detected in a wide range of host species, are viruses of which limited information is available about their pathogenic potential, ecology, or evolutionary characteristics. In this study, a molecular analysis of segment 2 encoding the PBV RNA-dependent RNA-polymerase (RdRp) in small ruminants with diarrhea in Turkey was undertaken. A total of 66 fecal samples or gut contents from diarrheic small ruminants including 55 sheep and 11 goats were screened. Four samples (6.06%), obtained from sheep in different farms, yielded the expected amplicon size for the genogroup I RdRp gene fragment, whereas no positivity was detected for genogroup II PBVs. Phylogenetic analysis revealed high levels of genetic diversity among the genogroup I PBVs. Additionally, all PBV infected sheep were also positive for rotavirus A. This study, reporting the presence of the PBVs in sheep Turkey for the first time, contributes to the molecular characterization and epidemiology of PBVs.

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Fig. 1

Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

The authors received no specific funding for this work.

Author information

Authors and Affiliations

  1. Department of Virology, Faculty of Veterinary Medicine, Ankara University, Diskapi, 06110, Ankara, Turkey

    Ilke Karayel-Hacioglu & Feray Alkan

  2. Department of Virology, Graduate School of Health Sciences, Ankara University, Sehit Omer Halisdemir Street, Diskapi, 06110, Ankara, Turkey

    Buket Gul

Authors
  1. Ilke Karayel-Hacioglu
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  2. Buket Gul
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  3. Feray Alkan
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Contributions

IKH together with BG conducted the experiments and performed the molecular biology and bioinformatic analyses (alignments, phylogeny). IKH drafted the manuscript. FA was responsible for the supervision of the study. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Ilke Karayel-Hacioglu.

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The authors declare that they have no conflict of interest.

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Not applicable.

Research involving human participants and/or animals

Symptomatic small ruminants were examined for clinical evaluation, and samples were collected by the field veterinarians. No ethical approval was required for this study.

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Edited by William Dundon.

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Supplementary Information

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11262_2022_1894_MOESM1_ESM.jpg

Supplementary file1 (PDF 1519 kb). Fig. S1 The map showing the distribution of the samples according to sampled provinces. Blue and grey colors indicate the provinces where positive and negative samples were detected, respectively

Supplementary file2 (PDF 300 kb).

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Karayel-Hacioglu, I., Gul, B. & Alkan, F. Molecular characterization of picobirnaviruses in small ruminants with diarrhea in Turkey. Virus Genes 58, 238–243 (2022). https://doi.org/10.1007/s11262-022-01894-9

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  • DOI: https://doi.org/10.1007/s11262-022-01894-9

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