Abstract
Newcastle disease virus with velogenic characteristics circulates in the poultry industry in Mexico and various other American countries. In Mexico, vaccine efficacy testing to obtain commercial registration is reliant on a challenge with a velogenic strain known colloquially as Chimalhuacan due to the site where it was isolated. In this paper, we performed a full genome sequencing of the Chimalhuacan strain. The strain belongs to Class II of APMV, particularly genotype V. The viral RNA genome is 15,192 nt in size and contains six genes: 3′ NP-P-M-F-HN-L 5′. The 3′ leader sequence is 55 nt in size and the 5′ trailer sequence 113 nt. The deduced amino acid sequence confirms a velogenic genotype with four basic amino acids at the cleavage site: 112RRQKR↓F117. In addition, evolutionary relatedness based on the gene sequence of the fusion protein indicates that this strain is the ancestor of the strains currently circulating in Mexico.
Similar content being viewed by others
References
M.A. Mayo, Arch. Virol. 147, 1655 (2002)
D.J. Alexander, in Disease of poultry, ed. by J.M. Saif, H.J. Barnes, J.R. Glisson, A.M. Fadly, L.R. McDougald, D.E. Swayne, I.A. Ames (Iowa State University Press, Ames, 2003), p. 541
R. Merino, H. Villegas, J.A. Quintana, N. Calderon, Int. J. Poult. Sci. 10, 713 (2011)
S.C. Courtney, L. Susta, D. Gomez, N.L. Hines, J.C. Pedersen, C.C. Brown, P.J. Miller, C.L. Afonso, J. Clin. Microbiol. 51, 508 (2013)
J.W. Walker, B.R. Heron, M.A. Mixon, Avian Dis. 17, 486 (1973)
R. Merino, H. Villegas, J.A. Quintana, N. Calderon, Vet. Res. Commun. 33, 1023 (2009)
Norma Oficial Mexicana. NOM-052-ZOO-1995. www.sagarpa.gob.mx. Accessed 27 Oct 2013
A.E. Absalón, A. Mariano-Matías, A. Vásquez-Marquez, A. Morales-Garzón, D.V. Cortés-Espinosa, R. Ortega-García, E. Lucio-Decanini, Virus Genes 45, 304 (2012)
K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei, S. Kumar, Mol. Biol. Evol. 28, 2731 (2011)
B.P.H. Peeters, O.S. de Leeuw, G. Koch, A.L.J. Gielkens, J. Virol. 73, 5001 (1999)
J.C.F.M. Dortmans, P.J.M. Rottier, G. Koch, B.P.H. Peeters, J. Gen. Virol. 92, 336 (2011)
D.G. Diel, L.H. da Silva, H. Liu, Z. Wang, P.J. Miller, C.L. Afonso, Infect. Genet. Evol. 12, 1770 (2012)
L. Susta, K.R. Hamal, P.J. Miller, S. Cárdenas-Garcia, C.C. Brown, J.C. Pedersen, V. Gongora, C.L. Afonso, J. Clin. Microbiol. 52, 1382 (2014)
F. Perozo, R. Merino, C.L. Afonso, P. Villegaz, N. Calderon, Avian Dis. 52, 472 (2008)
N.L. Hines, M.L. Killian, J.C. Pedersen, M.M. Reising, N.A. Mosos, C. Mathieu-Benson, C.L. Miller, Avian Dis. 56, 387 (2012)
Acknowledgments
We are grateful to Roberto Ortega and Raúl Montalvo for technical support. Private funding from Investigación Aplicada S.A. de C.V supported this study.
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Absalón, A.E., Mariano-Matías, A., García, L.J. et al. Complete genome analysis of velogenic Newcastle disease virus reference strain “Chimalhuacan”: evolution of viral lineages in Mexico. Virus Genes 49, 233–236 (2014). https://doi.org/10.1007/s11262-014-1082-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11262-014-1082-8