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Genomic sequence analysis of the Illinois strain of the Agrotis ipsilon multiple nucleopolyhedrovirus

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Abstract

The Agrotis ipsilon multiple nucleopolyhedrovirus (AgipMNPV) is a group II nucleopolyhedrovirus (NPV) from the black cutworm, A. ipsilon, with potential as a biopesticide to control infestations of cutworm larvae. The genome of the Illinois strain of AgipMNPV was completely sequenced. The AgipMNPV genome was 155,122 nt in size and contained 163 open reading frames (ORFs), including 61 ORFs found among all lepidopteran baculoviruses sequenced to date. Phylogenetic inference placed AgipMNPV in a clade with group II NPVs isolated from larvae of Agrotis and Spodoptera species. Though closely related to the Agrotis segetum NPV (AgseNPV), AgipMNPV was found to be missing 15 ORFs present in the AgseNPV genome sequence, including two of the three AgseNPV enhancin genes. Remarkably few polymorphisms were identified in the AgipMNPV sequence even though an uncloned field isolate of this virus was sequenced. A genotype characterized by a 128-bp deletion in the ecdysteroid UDP-glucosyltransferase gene (egt) was identified in the AgipMNPV field isolate and among clonal isolates of AgipMNPV. The deletion in egt was not associated with differences in budded virus or occluded virus production among AgipMNPV clones in cell culture.

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References

  1. B·C. Bonning, in Comprehensive Molecular Insect Science, ed. by L. Gilbert, K. Iatrou, S·S. Gill (Elsevier, Amsterdam; Boston, 2005), pp. 233–269

  2. D.A. Theilmann, G.W. Blissard, B. Bonning, J.A. Jehle, D.R. O’Reilly, G.F. Rohrmann, S. Thiem, J.M. Vlak. in Virus Taxonomy-Classification and Nomenclature of Viruses 8th Report of the International Committee on the Taxonomy of Viruses, ed. by C.X. Fauquet, M.A. Mayo, J. Maniloff, U. Desselberger, L.A. Ball (Elsevier, Amsterdam, 2005), pp. 177–185

  3. J.A. Jehle, G.W. Blissard, B.C. Bonning, J.S. Cory, E.A. Herniou, G.F. Rohrmann, D.A. Theilmann, S.M. Thiem, J.M. Vlak, Arch. Virol. 151, 1257–1266 (2006). doi:https://doi.org/10.1007/s00705-006-0763-6

    Article  CAS  Google Scholar 

  4. T.A. Kost, J.P. Condreay, D.L. Jarvis, Nat. Biotechnol. 23, 567–575 (2005). doi:https://doi.org/10.1038/nbt1095

    Article  CAS  PubMed Central  Google Scholar 

  5. M.D. Summers, Adv. Virus Res. 68, 3–73 (2006). doi:https://doi.org/10.1016/S0065-3527(06)68001-9

    Article  CAS  Google Scholar 

  6. F. Moscardi, Annu. Rev. Entomol. 44, 257–289 (1999). doi:https://doi.org/10.1146/annurev.ento.44.1.257

    Article  CAS  Google Scholar 

  7. N. van Beek, D.C. Davis, Methods Mol. Biol. 388, 367–378 (2007). doi:https://doi.org/10.1007/978-1-59745-457-5-19

    Article  Google Scholar 

  8. A.J. Boughton, R.L. Harrison, L.C. Lewis, B.C. Bonning, J. Invertebr. Pathol. 74, 289–294 (1999). doi:https://doi.org/10.1006/jipa.1999.4901

    Article  CAS  Google Scholar 

  9. R.W. Rings, F.J. Arnold, B.A. Johnson, B.C. Bonning, Bull. Entomol. Soc. Am. 21, 229–234 (1975)

    Google Scholar 

  10. A.J. Boughton, L.C. Lewis, J. Econ. Entomol. 94, 1045–1052 (2001)

    Article  CAS  Google Scholar 

  11. C.A. Prater, C.T. Redmond, W. Barney, B.C. Bonning, D.A. Potter, J. Econ. Entomol. 99, 1129–1137 (2006)

    Article  Google Scholar 

  12. D.A. Potter, A. Bixby, Green Sect. Rec. 46, 11–13 (2008)

    Google Scholar 

  13. S. El-Salamouny, M. Lange, M. Jutzi, J. Huber, J.A. Jehle, J. Invertebr. Pathol. 84, 75–82 (2003). doi:https://doi.org/10.1016/j.jip.2003.08.005

    Article  CAS  Google Scholar 

  14. A.K. Jakubowska, S.A. Peters, J. Ziemnicka, J.M. Vlak, M.M. van Oers, J. Gen. Virol. 87, 537–551 (2006). doi:https://doi.org/10.1099/vir.0.81461-0

    Article  CAS  Google Scholar 

  15. D.R. O’Reilly, L.K. Miller, V.A. Luckow, Baculovirus Expression Vectors (W·H. Freeman and Company, New York, 1992)

    Google Scholar 

  16. J.M. Slack, S.D. Lawrence, Methods Cell Sci. 24, 155–163 (2002). doi:https://doi.org/10.1023/A:1024413604663

    Article  CAS  Google Scholar 

  17. R.L. Harrison, D.E. Lynn, J. Invertebr. Pathol. 99, 28–34 (2008). doi:https://doi.org/10.1016/j.jip.2008.02.015

    Article  CAS  Google Scholar 

  18. R.L. Harrison, B. Puttler, H.J. Popham, J. Gen. Virol. 89, 775–790 (2008). doi:https://doi.org/10.1099/vir.0.83566-0

    Article  CAS  Google Scholar 

  19. J.D. Thompson, D.G. Higgins, T.J. Gibson, Nucleic Acids Res. 22, 4673–4680 (1994). doi:https://doi.org/10.1093/nar/22.22.4673

    Article  CAS  PubMed Central  Google Scholar 

  20. K. Tamura, J. Dudley, M. Nei, S. Kumar, Mol. Biol. Evol. 24, 1596–1599 (2007). doi:https://doi.org/10.1093/molbev/msm092

    Article  CAS  Google Scholar 

  21. J. Kuzio, M.N. Pearson, S.H. Harwood, C.J. Funk, J.T. Evans, J.M. Slavicek, G.F. Rohrmann, Virology 253, 17–34 (1999). doi:https://doi.org/10.1006/viro.1998.9469

    Article  CAS  Google Scholar 

  22. W.F. IJkel, E.A. van Strien, J.G. Heldens, R. Broer, D. Zuidema, R.W. Goldbach, J.M. Vlak, J. Gen. Virol. 80, 3289–3304 (1999)

    Article  CAS  Google Scholar 

  23. Y. Pang, J. Yu, L. Wang, X. Hu, W. Bao, G. Li, C. Chen, H. Han, S. Hu, H. Yang, Virology 287, 391–404 (2001). doi:https://doi.org/10.1006/viro.2001.1056

    Article  CAS  Google Scholar 

  24. X. Chen, I.J. WF, R. Tarchini, X. Sun, H. Sandbrink, H. Wang, S. Peters, D. Zuidema, R.K. Lankhorst, J.M. Vlak, Z. Hu, J. Gen. Virol. 82, 241–257 (2001)

    Article  CAS  Google Scholar 

  25. X. Chen, W.J. Zhang, J. Wong, G. Chun, A. Lu, B.F. McCutchen, J.K. Presnail, R. Herrmann, M. Dolan, S. Tingey, Z.H. Hu, J.M. Vlak, J. Gen. Virol. 83, 673–684 (2002)

    Article  Google Scholar 

  26. Q. Li, C. Donly, L. Li, L.G. Willis, D.A. Theilmann, M. Erlandson, Virology 294, 106–121 (2002). doi:https://doi.org/10.1006/viro.2001.1313

    Article  CAS  Google Scholar 

  27. L. Li, C. Donly, Q. Li, L.G. Willis, B.A. Keddie, M.A. Erlandson, D.A. Theilmann, Virology 297, 226–244 (2002). doi:https://doi.org/10.1006/viro.2002.1411

    Article  CAS  Google Scholar 

  28. M. Nakai, C. Goto, W. Kang, M. Shikata, T. Luque, Y. Kunimi, Virology 316, 171–183 (2003). doi:https://doi.org/10.1016/j.virol.2003.08.002

    Article  CAS  Google Scholar 

  29. L.G. Willis, R. Seipp, T.M. Stewart, M.A. Erlandson, D.A. Theilmann, Virology 338, 209–226 (2005). doi:https://doi.org/10.1016/j.virol.2005.04.041

    Article  CAS  Google Scholar 

  30. C.X. Zhang, X.C. Ma, Z.J. Guo, Virology 333, 190–199 (2005). doi:https://doi.org/10.1016/j.virol.2004.12.028

    Article  CAS  Google Scholar 

  31. L. Li, Q. Li, L.G. Willis, M. Erlandson, D.A. Theilmann, C. Donly, J. Gen. Virol. 86, 91–105 (2005). doi:https://doi.org/10.1099/vir.0.80488-0

    Article  CAS  Google Scholar 

  32. M.M. van Oers, M.H. Abma-Henkens, E.A. Herniou, J.C. de Groot, S. Peters, J.M. Vlak, J. Gen. Virol. 86, 2069–2080 (2005). doi:https://doi.org/10.1099/vir.0.80964-0

    Article  Google Scholar 

  33. X.C. Ma, J.Y. Shang, Z.N. Yang, Y.Y. Bao, Q. Xiao, C.X. Zhang, Virology 360, 235–246 (2007). doi:https://doi.org/10.1016/j.virol.2006.10.024

    Article  CAS  Google Scholar 

  34. H. Xiao, Y. Qi, Virus Genes 35, 845–856 (2007). doi:https://doi.org/10.1007/s11262-007-0106-z

    Article  CAS  Google Scholar 

  35. J.L. Wolff, F.H. Valicente, R. Martins, J.V. Oliveira, P.M. Zanotto, J. Gen. Virol. 89, 1202–1211 (2008). doi:https://doi.org/10.1099/vir.0.83581-0

    Article  CAS  Google Scholar 

  36. M.D. Ayres, S.C. Howard, J. Kuzio, M. Lopez-Ferber, R.D. Possee, Virology 202, 586–605 (1994). doi:https://doi.org/10.1006/viro.1994.1380

    Article  CAS  Google Scholar 

  37. T. Luque, R. Finch, N. Crook, D.R. O’Reilly, D. Winstanley, J. Gen. Virol. 82, 2531–2547 (2001)

    Article  CAS  Google Scholar 

  38. T. Hayakawa, R. Ko, K. Okano, S.I. Seong, C. Goto, S. Maeda, Virology 262, 277–297 (1999). doi:https://doi.org/10.1006/viro.1999.9894

    Article  CAS  Google Scholar 

  39. R.L. Harrison, H.J. Popham, Virus Genes 36, 565–581 (2008). doi:https://doi.org/10.1007/s11262-008-0218-0

    Article  CAS  Google Scholar 

  40. C.H. Ahrens, R.L. Russell, C.J. Funk, J.T. Evans, S.H. Harwood, G.F. Rohrmann, Virology 229, 381–399 (1997). doi:https://doi.org/10.1006/viro.1997.8448

    Article  CAS  Google Scholar 

  41. Q. Fan, S. Li, L. Wang, B. Zhang, B. Ye, Z. Zhao, L. Cui, Virology 366, 304–315 (2007). doi:https://doi.org/10.1016/j.virol.2007.04.027

    Article  CAS  Google Scholar 

  42. Z.M. Nie, Z.F. Zhang, D. Wang, P.A. He, C.Y. Jiang, L. Song, F. Chen, J. Xu, L. Yang, L.L. Yu, J. Chen, Z.B. Lv, J.J. Lu, X.F. Wu, Y.Z. Zhang, BMC Genomics 8, 248 (2007). doi:https://doi.org/10.1186/1471-2164-8-248

    Article  PubMed Central  Google Scholar 

  43. C.L. Afonso, E.R. Tulman, Z. Lu, C.A. Balinsky, B.A. Moser, J.J. Becnel, D.L. Rock, G.F. Kutish, J. Virol. 75, 11157–11165 (2001). doi:https://doi.org/10.1128/JVI.75.22.11157-11165.2001

    Article  CAS  PubMed Central  Google Scholar 

  44. J.G. de Jong, H.A. Lauzon, C. Dominy, A. Poloumienko, E.B. Carstens, B.M. Arif, P.J. Krell, J. Gen. Virol. 86, 929–943 (2005). doi:https://doi.org/10.1099/vir.0.80490-0

    Article  Google Scholar 

  45. M.M. van Oers, J.M. Vlak, Curr. Drug Targets 8, 1051–1068 (2007). doi:https://doi.org/10.2174/138945007782151333

    Article  Google Scholar 

  46. C.B. McCarthy, D.A. Theilmann, Virology 375, 277–291 (2008). doi:https://doi.org/10.1016/j.virol.2008.01.039

    Article  CAS  Google Scholar 

  47. Z.H. Hu, B.M. Arif, F. Jin, J.W. Martens, X.W. Chen, J.S. Sun, D. Zuidema, R.W. Goldbach, J.M. Vlak, J. Gen. Virol. 79, 2841–2851 (1998)

    Article  CAS  Google Scholar 

  48. S. Hilton, D. Winstanley, J. Gen. Virol. 88, 1496–1504 (2007). doi:https://doi.org/10.1099/vir.0.82760-0

    Article  CAS  Google Scholar 

  49. R.D. Possee, G.F. Rohrmann, in The Baculoviruses, ed. by L.K. Miller (Plenum, New York, 1997), pp. 109–140

  50. T. Hong, S.C. Braunagel, M.D. Summers, Virology 204, 210–222 (1994). doi:https://doi.org/10.1006/viro.1994.1525

    Article  CAS  Google Scholar 

  51. C.J. Chen, S.M. Thiem, Virology 227, 88–95 (1997). doi:https://doi.org/10.1006/viro.1996.8341

    Article  CAS  Google Scholar 

  52. A. Lu, L.K. Miller, J. Virol. 69, 6265–6272 (1995)

    CAS  PubMed  PubMed Central  Google Scholar 

  53. D. Dall, T. Luque, D. O’Reilly, Bioessays 23, 184–193 (2001). doi:10.1002/1521-1878(200102)23:2<184::AID-BIES1026>3.0.CO;2-H

    Article  CAS  Google Scholar 

  54. D.K. Bideshi, S. Renault, K. Stasiak, B.A. Federici, Y. Bigot, J. Gen. Virol. 84, 2531–2544 (2003). doi:https://doi.org/10.1099/vir.0.19256-0

    Article  CAS  Google Scholar 

  55. L.M. Iyer, E.V. Koonin, L. Aravind, Genome Biol. 3, RESEARCH0012 (2002)

    Article  PubMed Central  Google Scholar 

  56. E.A. Zemskov, W. Kang, S. Maeda, J. Virol. 74, 6784–6789 (2000). doi:https://doi.org/10.1128/JVI.74.15.6784-6789.2000

    Article  CAS  PubMed Central  Google Scholar 

  57. J.S. Cory, B.M. Green, R.K. Paul, F. Hunter-Fujita, J. Invertebr. Pathol. 89, 101–111 (2005). doi:https://doi.org/10.1016/j.jip.2005.03.008

    Article  Google Scholar 

  58. J.S. Cory, J.H. Myers, Annu. Rev. Ecol. Evol. Syst. 34, 239–272 (2003). doi:https://doi.org/10.1146/annurev.ecolsys.34.011802.132402

    Article  Google Scholar 

  59. D.R. O’Reilly, M.R. Brown, L.K. Miller, Insect Biochem. Mol. Biol. 22, 313–320 (1992). doi:https://doi.org/10.1016/0965-1748(92)90069-Q

    Article  Google Scholar 

  60. D.R. O’Reilly, O.W. Howarth, H.H. Rees, L.K. Miller, Insect Biochem. 21, 795–801 (1991). doi:https://doi.org/10.1016/0020-1790(91)90121-T

    Article  Google Scholar 

  61. D.R. O’Reilly, R.S. Hails, T.J. Kelly, J. Invertebr. Pathol. 72, 269–275 (1998). doi:https://doi.org/10.1006/jipa.1998.4785

    Article  Google Scholar 

  62. D.R. O’Reilly, L.K. Miller, Science 245, 1110–1112 (1989). doi:https://doi.org/10.1126/science.2505387

    Article  Google Scholar 

  63. K.R. Wilson, D.R. O’Reilly, R.S. Hails, J.S. Cory, Biol. Control 19, 57–63 (2000). doi:https://doi.org/10.1006/bcon.2000.0841

    Article  CAS  Google Scholar 

  64. S. Kumar, L.K. Miller, Virus Res. 7, 335–349 (1987). doi:https://doi.org/10.1016/0168-1702(87)90047-5

    Article  CAS  Google Scholar 

  65. D.R. O’Reilly, A.L. Passarelli, I.F. Goldman, L.K. Miller, J. Gen. Virol. 71, 1029–1037 (1990). doi:https://doi.org/10.1099/0022-1317-71-5-1029

    Article  Google Scholar 

  66. O. Simon, T. Williams, M. Lopez-Ferber, P. Caballero, Appl. Environ. Microbiol. 70, 5579–5588 (2004). doi:https://doi.org/10.1128/AEM.70.9.5579-5588.2004

    Article  CAS  PubMed Central  Google Scholar 

  67. B. Beames, M.D. Summers, Virology 168, 344–353 (1989). doi:https://doi.org/10.1016/0042-6822(89)90275-4

    Article  CAS  Google Scholar 

  68. R.L. Harrison, M.D. Summers, J. Gen. Virol. 76, 1451–1459 (1995). doi:https://doi.org/10.1099/0022-1317-76-6-1451

    Article  CAS  Google Scholar 

  69. D.S. Bischoff, J.M. Slavicek, J. Virol. 71, 8133–8140 (1997)

    CAS  PubMed  PubMed Central  Google Scholar 

  70. H.J. Popham, D.S. Bischoff, J.M. Slavicek, J. Virol. 75, 8639–8648 (2001). doi:https://doi.org/10.1128/JVI.75.18.8639-8648.2001

    Article  CAS  PubMed Central  Google Scholar 

  71. J.H. Zhang, T. Ohkawa, J.O. Washburn, L.E. Volkman, J. Gen. Virol. 86, 1619–1627 (2005). doi:https://doi.org/10.1099/vir.0.80930-0

    Article  CAS  Google Scholar 

  72. R. Lapointe, H.J. Popham, U. Straschil, D. Goulding, D.R. O’Reilly, J.A. Olszewski, J. Virol. 78, 6439–6448 (2004). doi:https://doi.org/10.1128/JVI.78.12.6439-6448.2004

    Article  CAS  PubMed Central  Google Scholar 

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Acknowledgements

The author wishes to thank Tad Sonstegard (USDA-ARS, Bovine Functional Genomics Laboratory, Beltsville, MD) for the use of his GeneMachines Hydroshear, Jing Hu and Dan Rowley (USDA-ARS, Invasive Insect Biocontrol and Behavior Laboratory, Beltsville, MD) for assistance with sequencing, and anonymous referees for a critical review of the manuscript. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture.

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  1. Invasive Insect Biocontrol and Behavior Laboratory, USDA Agricultural Research Service, Plant Sciences Institute, Building 011A, Room 214, BARC-W, 10300 Baltimore Avenue, Beltsville, MD, 20705, USA

    Robert L. Harrison

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The nucleotide sequence data reported in this article have been submitted to the GenBank nucleotide sequence database and have been assigned the accession number EU839994.

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Harrison, R.L. Genomic sequence analysis of the Illinois strain of the Agrotis ipsilon multiple nucleopolyhedrovirus . Virus Genes 38, 155–170 (2009). https://doi.org/10.1007/s11262-008-0297-y

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