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Role of the 25–26 nt siRNA in the resistance of transgenic Prunus domestica graft inoculated with plum pox virus

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Abstract

The reaction of a genetically engineered plum clone (C5) resistant to plum pox virus (PPV) by graft inoculation with the virus was evaluated. The resistance in this clone has been demonstrated to be mediated through post-transcriptional gene silencing (PTGS). A single C5 plant out of 30 plants inoculated with PPV M strain by double chip-budding showed mild diffuse mosaic ‘Sharka’ symptom at the bottom section of the scion. The upper leaves of this PPV-infected C5 plant remained symptomless and the virus was not detected in them by either DAS-ELISA or RT-PCR. An RNA silencing associated small interfering RNA duplex, siRNA (21–26 nt), was detected in non-inoculated C5 plants and in the portions of inoculated C5 plant in which PPV could not be detected. In the PPV-infected portion of the C5 plant and in C6 PPV susceptible plants only the ∼21–22 nt siRNAs was detected. Cytosine-methylation was confirmed in C5 plants both uninfected and showing PPV symptoms. The 25–26 nt siRNA normally present in C5 was absent in PPV-infected C5 tissues confirming the critical role of this siRNA in the resistance of clone C5 to PPV infection. We also show that this PPV infection was limited and transient. It was only detected in one plant at one of four post-dormancy sampling dates and did not appear to affect the overall PPV resistance of the C5 clone.

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Acknowledgment

This work is sponsored by EU contract “TRANSVIR” QLK3-2002-0140.

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Author notes

  1. Jiban Kumar Kundu

    Present address: Department of Virology, Crop Research Institute, Drnovska 507, 161 06, Praha 6-Ruzyne, Czech Republic

Authors and Affiliations

  1. UMR, GDPP, Virologie, INRA-Bordeaux, BP-81, Villenave d’Ornon, 33883, France

    Jiban Kumar Kundu, Pascal Briard, Jean Michel Hily & Michel Ravelonandro

  2. USDA-ARS Appalachian Fruit Research Station, 2217 Wiltshire Rd., Kearneysville, WV, 25430, USA

    Jean Michel Hily & Ralph Scorza

Authors
  1. Jiban Kumar Kundu
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  2. Pascal Briard
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  3. Jean Michel Hily
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  4. Michel Ravelonandro
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  5. Ralph Scorza
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Corresponding author

Correspondence to Michel Ravelonandro.

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Kundu, J.K., Briard, P., Hily, J.M. et al. Role of the 25–26 nt siRNA in the resistance of transgenic Prunus domestica graft inoculated with plum pox virus . Virus Genes 36, 215–220 (2008). https://doi.org/10.1007/s11262-007-0176-y

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  • DOI: https://doi.org/10.1007/s11262-007-0176-y

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