Abstract
The population structure and genetic diversity of Cucumber vein yellowing virus (CVYV) from Spain were estimated by analyses of partial nucleotide sequences of the P1-proteinase (P1-Pro), P3 protein (P3), and the coat protein (CP) coding regions. Analysis of 56 CVYV Spanish field isolates collected from 2001 to 2005 showed low genetic diversity (0.0026, 0.0013, and 0.0012 for the P1-Pro, P3, and CP regions, respectively). The ratio between nonsynonymous and synonymous substitutions was among the lowest found in a plant virus, indicating a strong negative selective pressure in the regions analyzed. Nonsynonymous nucleotide substitutions were only found within the P1-Pro regions, although these do not appear to have been selected with time. The results support the hypothesis that the Spanish CVYV population could derive from a single origin of recent introduction.
Similar content being viewed by others
References
E. Domingo, J.J. Holland, in The Evolutionary Biology of Viruses, ed. by S.S. Morse (Raven Press, New York, 1994), pp. 161–184
V.V. Dolja, J.C. Carrington, Semin. Virol. 3, 315–326 (1992)
M. Gibbs, in Molecular Basis of Virus Evolution, ed. by A.J. Gibbs, C.H. Calisher, F. García-Arenal (Cambridge University Press, Cambridge, 1995), pp. 351–368
E.V. Koonin, V.V. Dolja, Crit. Rev. Biochem. Mol. Biol. 28, 375–430 (1993)
M.J. Roossinck, Ann. Rev. Phytopathol. 35, 191–209 (1997)
F. García-Arenal, A. Fraile, J.M. Malpica, Ann. Rev. Phytopathol. 39, 157–186 (2001)
I.M. Moreno, J.R. Thompson, F. García-Arenal, J. Gen. Virol. 85, 749–759 (2004)
H. Lecoq, C. Desbiez, B. Delécolle, S. Cohen, A. Mansour, J. Gen. Virol. 81, 2289–2293 (2000)
D. Janssen, G. Martín, L. Velasco, P. Gómez, E. Segundo, L. Ruiz, I.M. Cuadrado, Arch. Virol. 150, 1439–1447 (2005)
S. Cohen, F.E. Nitzany, Phytopathol. Medit. 1, 44–46 (1960)
I. Harpaz, S. Cohen, J. Phytopathol. 54, 240–248 (1965)
A. Mansour, A. Al-Musa, J. Phytopathol. 137, 73–78 (1993)
A.M. Al Musa, S.J. Qusus, A.N. Mansour, Plant Dis. 69, 361 (1985)
M.A. Yimaz, M. Ozaslan, D. Ozaslan, Plant Dis. 73, 610–610 (1989)
I.M. Cuadrado, D. Janssen, L. Velasco, L. Ruiz, E. Segundo, Plant Dis. 85, 336 (2001)
D. Janssen, I.M. Cuadrado, EWSN Newslett. 10, 1–2 (2001)
D. Janssen, L. Ruiz, L. Velasco, E. Segundo, G. Martin, I.M. Cuadrado, Z. Pflanzenk. Pflanzen. 110, 90–91 (2003)
D. Louro, A. Quinot, E. Neto, J.E. Fernandes, D. Marian, M. Vecchiati, P. Caciagli, A.M. Vaira, Plant Pathol. 53, 241–241 (2004)
L.C. Papayiannis, N. Ioannou, I.N. Boubourakas, C.I. Dovas, N.I. Katis, B.W. Falk, J. Phytopathol. 153, 530–535 (2005)
V.V. Dolja, V.P. Boyko, A.A. Agranovsky, E.V. Koonin, Virology 184, 79–86 (1991)
M.E. Aleman-Verdaguer, C. Goudou-Urbino, J. Dubern, R.N. Beachy, C. Fauquet, J. Gen. Virol. 78, 1253–1264 (1997)
K.C. Lee, S.M. Wong, DNA Sequence 9, 275–293 (1998)
C.W. Ward, D.D. Shukla, Intervirology 32, 269–296 (1991)
J. Sambrook, D. Russell, Molecular Cloning: A Laboratory Manual, 3rd edn. (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 2000), p. 999
J.D. Thompson, T.J. Gibson, F. Plewniak, F. Jeanmougin, D.G. Higgins, Nucleic Acids Res. 25, 4876–4882 (1997)
W.-H. Li, J. Mol. Evol. 36, 96–99 (1993)
P. Pamilo, N.O. Bianchi, Mol. Biol. Evol. 10, 271–281 (1993)
M. Kimura, J. Mol. Evol. 16, 111–120 (1980)
M. Nei, S. Kumar, Molecular Evolution, Phylogenetics (Oxford University Press, New York, 2000), p. 333
S. Kumar, K. Tamura, I.B. Jakobsen, M. Nei, Bioinformatics 17, 1244–1245 (2001)
M. Nei, Molecular Evolutionary Genetics (Columbia University Press, New York, 1987), p. 512
M. Nei, T. Gojobori, Mol. Biol. Evol. 3, 418–426 (1986)
Z. Yang, J.P. Bielawski, Trends Ecol. Evol. 15, 496–503 (2000)
T. Kekarainen, A. Merits, I. Oruetxebarria, M. Rajamaki, J.P. Valkonen, Arch. Virol. 144, 2355–2366 (1999)
I. Oruetxebarria, T. Kekarainen, C. Spetz, J.P. Valkonen, Phytopathology 90, 437–444 (2000)
P. Kong, L. Rubio, M. Polek, B.W. Falk, Virus Genes 21, 139–145 (2000)
L. Rubio, Y. Abou-Jawdah, H.X. Lin, B.W. Falk, J. Gen. Virol. 82, 929–933 (2001)
W.L. Schneider, M.J. Roossinck, J. Virol. 74, 3130–3134 (2000)
J.J. Holland, K. Spindler, F. Horodyski, E. Grabau, S. Nichol, S. VandePol, Science 215, 1577–1582 (1982)
L. Rubio, J. Soong, J. Kao, B.W. Falk, Phytopathology 89, 707–711 (1999)
T. Alicai, N.S. Fenby, R.W. Gibson, E. Adipala, H.J. Vetten, G.D. Foster, S.E. Seal, Plant Pathol. 48, 718–726 (1999)
S.B. Mukasa, P.R. Rubaihayo, J.P.T. Valkonen, Arch. Virol. 148, 487–496 (2003)
M. Bousalem, E.J.P. Douzery, D. Fargette, J. Gen. Virol. 81, 243–255 (2000)
B. Moury, C. Morel, E. Johansen, M. Jacquemond, J. Gen. Virol. 83, 2563–2573 (2002)
A. Fraile, J.M. Malpica, M.A. Aranda, E. Rodríguez-Cerezo, F. García-Arenal, Virology 223, 148–155 (1996)
A. Fraile, J.L. Alonso-Prados, M.A. Aranda, J.J. Bernal, J.M. Malpica, F. García-Arenal, J. Virol. 71, 934–940 (1997)
S. Sanchez-Campos, J.A. Díaz, F. Monci, E.R. Bejarano, J. Reina, J. Navas-Castillo, M.A. Aranda, E. Moriones, Phytopathology 92, 842–849 (2002)
Acknowledgments
The authors wish to thank Hervé Lecoq (INRA, Avignon-Montfavet) for his critical review of the manuscript. The present study was supported by research project PIA03/31 from I.F.A.P.A.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Janssen, D., Velasco, L., Martín, G. et al. Low genetic diversity among Cucumber vein yellowing virus isolates from Spain. Virus Genes 34, 367–371 (2007). https://doi.org/10.1007/s11262-006-0026-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11262-006-0026-3