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Evolution of 2B and 2C Genomic Parts of Species B Coxsackie Viruses. Phylogenetic Study and Comparison with Other Regions

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Abstract

Modern molecular approaches on the genome of enteroviruses’ circulating strains have established new data about the mechanism and significance of its evolution. In the present study, 46 enteroviruses isolates, belonging to HEV-B species and exhibiting distinct origin in geographical or chronological terms, were investigated concerning their primary structure and phylogeny. Two regions of the aforementioned strains genome, which have not been thoroughly investigated (2B and 5′ extreme of 2C) were amplified and sequenced for the first time. Phylogenetic and nucleotide analysis of the isolates’ fragments, along with representative prototype sequences, demonstrate that the classification scheme of monophyly and accordance with the genotype, which characterizes VP1 region, is seriously disturbed. Moreover, the phylogenetic trees constructed from adjacent regions of the genome appear radically incongruent suggesting that the parameters that affect these portions are different or act in a different extent. Our study results an additional step in the study of enteroviruses evolution and inheritance, by investigating unstudied regions of newly sequenced strains and revealing that the primary structure and phylogeny of them is different not only comparably to the structural genome but also from one to another.

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Acknowledgements

The authors would like to thank Prof. Glyn Stanway, Dr Francis Delpeyroux, Dr Marianna Combiescu, Dr G. Papageorgiou and Prof. George Berensci for the kind provision of many CAV and CBV strains. The present work was co funded by the European Union (75%) and the Greek Ministry of Education (25%) under the framework of the Education and Initial Vocational Training Program “Pythagoras II.” Research project: “Molecular detection of Enteroviruses in clinical samples and the environment. Implications for the public health.”

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Authors and Affiliations

  1. Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Panepistimioupolis, Athens, Greece

    Eugenia Bolanaki, Lukas Margaritis & Theodoros Katsorchis

  2. Department of Biochemistry & Biotechnology, Microbiology–Virology Laboratory, University of Thessaly, 26 Ploutonos & Aeolou str, 41221, Larisa, Greece

    Christine Kottaridi & Panayotis Markoulatos

  3. Virology Laboratory, Hellenic Pasteur Institute, 127, Vasilissis Sofias Ave, 115 21, Athens, Greece

    Eugenia Bolanaki

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  1. Eugenia Bolanaki
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  2. Christine Kottaridi
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Correspondence to Panayotis Markoulatos.

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Bolanaki, E., Kottaridi, C., Markoulatos, P. et al. Evolution of 2B and 2C Genomic Parts of Species B Coxsackie Viruses. Phylogenetic Study and Comparison with Other Regions. Virus Genes 32, 249–259 (2006). https://doi.org/10.1007/s11262-005-6909-x

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