Skip to main content
SpringerLink
Log in

Identification of the Apoptosis Inhibitor Gene p49 of Spodoptera litura Multicapsid Nucleopolyhedrovirus

  • Published:
Virus Genes Aims and scope Submit manuscript

Abstract

Baculoviruses possess two types of genes that suppressed apoptosis, p35 and inhibitor of apoptosis (iap). Computer-assisted analysis indicated that Spodoptera litura multicapsid nucleopolyhedrovirus (SpltMNPV) ORF55 (designated as the p49 gene) display 79 and 31% amino acid identity with Spodoptera littoralis (Spli)MNPV P49 and Autographa californica (Ac)MNPV P35, respectively, Splt MNPV putative P49 contains a peptide cleavage site TVTDG recognized by death caspases. In marker rescue assay, Splt-p49 was able to suppress apoptosis induced by infection of a mutant AcMNPV deficient in p35 and rescue the mutant virus replication from apoptosis in Sf-9 cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. L.K. Miller (1997) J Cell Physiol 173 178–182

    Google Scholar 

  2. P.A. Hershberger D.J. LaCount P.D. Friesen (1994) J Virol 68 3467–3477

    Google Scholar 

  3. B.A. Hay T Wolff G.M. Rubin (1994) Development 120 2121–2129

    Google Scholar 

  4. A. Sugimoto P.D. Friesen J.H. Rothman (1994) EMBO J 13 2023–2038

    Google Scholar 

  5. I. Martinou P.A. Fernandez M. Missotten E. White B. Allet R. Sadoul J.C. Martinou (1995) J Cell Biol 128 201–208

    Google Scholar 

  6. Q. Zhou J.F. Krebs S.J. Snipas A. Price E.S. Alnemri K.J. Tomaselli G.S. Salvesen (1998) Biochemistry 37 10757–10765

    Google Scholar 

  7. J. Bertin S.M. Mendrys D.J. LaCount S. Gaur J.F. Krebs R.C. Armstrong K.J. Tomaselli P.D. Friesen (1996) J Virol 70 6251–6259

    Google Scholar 

  8. D. Xue H.R. Horvitz (1995) Nature 377 248–251

    Google Scholar 

  9. D.R. Green J.C. Reed (1998) Science 281 1309–1312

    Google Scholar 

  10. Z. Pei G. Reske Q. Huang B.D. Hammock Y. Qi N. Chejanovsky (2002) J Bio Chem 277 48677–48684

    Google Scholar 

  11. Q. Du D. Lehay O. Faktor Y Qi N. Chejanovsky (1999) J Virol 73 1278–1285

    Google Scholar 

  12. S.J. Zoog J.J. Schiller J.A. Wetter N. Chejanovsky P.D. Friesen (2002) EMBO J 21 5130–5140

    Google Scholar 

  13. T. Luque R. Finch N. Crook D.R. O’Reilly D. Winstanley (2001) J Gen Virol 82 2531–2547

    Google Scholar 

  14. T. Maguire P. Harrison O. Hyink J. Kalmakoff V.K. Ward (2000) J Gen Virol 81 2803–2011

    Google Scholar 

  15. M. Ikeda K. Yanagimoto M. Kobayashi (2004) Virology 321 359–371

    Google Scholar 

  16. L. Vilaplana D.R. O’Reilly (2003) Virus Res 92 107–111

    Google Scholar 

  17. Q. Liu Y. Qi N. Chejanovsky (2003) Virus Genes 26 143–149

    Google Scholar 

  18. Pang Y. in Pu Z.L. (ed.), Insect Pathology. Guangdong Science and Technology Press, Guangzhou, 1994, pp. 85–216

  19. Q.J. Chen Y. Pang G.H. Li (1998) J Wuhan Univ Technol 44 183

    Google Scholar 

  20. Pang Y., in Jin S.B. (ed.), Study and Application of Insect Pathogens. Shanxi Science and Technology Press, Taiyuan, 1998, pp. 369–496

  21. Y. Pang J.X. Yu L.G. Wang X.H. Hu W.D. Bao G. Li C. Chen H. Han S. Hu H. Yang (2001) Virology 287 391–404

    Google Scholar 

  22. J.D. Thompson D.G. Higgins T.J. Gibson (1994) Nucleic Acids Res 22 4673–4680

    Google Scholar 

  23. E. Harlow D. Lane (1988) Antibodies: A Laboratory Manual Cold Spring Harbor NewYork

    Google Scholar 

  24. M. Herrmann H.M. Lorenz R. Voll M. Grunke W. Woith J.R. Kalden (1994) Nucleic Acids Res 22 5506–5507

    Google Scholar 

  25. J. Sambrook E.F. Fritsch T. Maniatis (1989) Molecular Cloning: A Laboratory Manual Cold Spring Harbor NewYork

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. State Key Laboratory for Biocontrol and Institute of Entomology, Zhongshan University, 510275, Guangzhou, P.R. China

    Mei Yu, Zhaofei Li, Kai Yang, Tiehao Lin, Yingxue Gong, Lijing Pan & Yi Pang

  2. Life Science School, Foshan University, 528231, Foshan, P.R. China

    Mei Yu

Authors
  1. Mei Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhaofei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kai Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tiehao Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yingxue Gong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Lijing Pan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yi Pang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yi Pang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yu, M., Li, Z., Yang, K. et al. Identification of the Apoptosis Inhibitor Gene p49 of Spodoptera litura Multicapsid Nucleopolyhedrovirus. Virus Genes 31, 145–151 (2005). https://doi.org/10.1007/s11262-005-1786-x

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11262-005-1786-x

Keywords

Search

Navigation