Abstract
The family Cyathocotylidae trematode is a world-widely distributed parasite whose adults are mainly found in fish, reptiles, birds, and mammals in both freshwater and marine environments. However, little is known of the prevalence of these trematode in China. For the first time, we found Cyathocotylidae trematode in the Wuhu area. Therefore, we investigated the prevalence of metacercariae in Carassius auratus from Zhang Lake, Kui Lake, Qingyi River, and Yangtze River (Wuhu Section) in Wuhu area. A total of 392 one-year-old C. auratus were tested in January, April, July, and October 2019, respectively. After the fish were euthanized, the back, chest, and tail muscles were used for preliminary screening for the presence of metacercariae by direct compression method. Metacercariae were isolated by the artificial digestion method and then morphologically and molecularly identified by cox1 (642 bp) and ITS2 (418 bp) sequences amplification by PCR. Based on morphological features and sequence analysis, they were identified as cyathocotylid metacercariae. The four water sources have different degrees of prevalence throughout the year, the prevalence ranges from 28.45% (29/102) in Yangtze River to 50.55% (46/91) in Zhang Lake, with an average prevalence of 40.56% (159/392). There were also differences in the prevalence in different months: The highest occurred in July, which was 81.63% (80/98), while the lowest occurred in January, which was 8.89% (8/90). This study can provide basic data for the prevention and control of cyathocotylid trematode in this area.
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Data availability
The cox1 and ITS2 data presented in this study can be found in GenBank under accession number ON411423 – ON411427 and ON261172 – ON261176.
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Acknowledgements
The authors gratefully acknowledge Prof. Chaopin Li (Wannan Medical College) for his help with morphological identification. The authors also are gratefully acknowledge the Central Laboratory of Wannan Medical College for providing experimental facilities and conditions.
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This work was supported by the Key Projects of Natural Science Research of Universities in Anhui Province (grant no. KJ2019A0403).
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All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by FJ, WZ, YP, XY, JC, ND, MZ, and SG. The first draft of the manuscript was written by FJ and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Jiang, F., Zhang, W., Pan, Y. et al. Identification and prevalence investigation of cyathocotylid trematode (Trematoda: Digenea: Cyathocotylidae) in Carassius auratus in Wuhu, China. Vet Res Commun 47, 1185–1193 (2023). https://doi.org/10.1007/s11259-022-10049-y
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DOI: https://doi.org/10.1007/s11259-022-10049-y