Abstract
Therapeutic efficacy of a new ‘Indigenous vaccine’ prepared from native highly pathogenic ‘Indian Bison Type’ genotype of Mycobacterium avium subspecies paratuberculosis (MAP) of goat origin has been evaluated with respect to control of clinical Johne’s disease in naturally infected Mehsana breed of goat in North Gujarat. Fifty goats from Sheep and Goats Research Station, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, were randomly divided into 2 groups viz.,‘Vaccinated’(n = 35) and ‘Control’(n = 15). After vaccination, goats were monitored for physical condition, morbidity, mortality, body weights, shedding of MAP in feces, internal condition, gross lesions and humoral immune responses up to 120 days (at each interval of 30 days). At the end of 120 days trial, there was marked overall improvement in physical condition and body weights of vaccinated goats as compared to ‘Control’ goats. Vaccinated goats gained significantly (P < 0.05) higher body weights, hardly exhibited any lesions characteristic of JD, had significantly higher (P < 0.01) antibody titers and shedding of MAP was significantly (P < 0.01) reduced. Few of the vaccinated goats were positive for MAP DNA in faecal PCR and blood PCR before vaccination. However, all were found as negative at 120 days post vaccination (DPV). Overall vaccine exhibited effective in restriction of MAP infection and significant improvement in production parameters and reduction in mortality and morbidity due to JD. The trial in the herd will be continued.
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References
Barad DB (2009) Comparative evaluation of molecular, serological and conventional diagnostic methods for Johne’s disease in goats. M.V.Sc. thesis, Sardarkrushinagar Dantiwada Agriculture University. Sardarkrushinagar. Gujarat
Collins MT (2002) Interpretation of a commercial bovine paratuberculosis enzyme-linked immunosorbent assay by using likelihood ratio. Clin Diagn Lab Immun 9(6):1367–1371
Corpa JM, Pe’erez V, Garc’ıa Mar’ın JF (2000) Differences in the immune responses in lambs and kids vaccinated against paratuberculosis, according to the age of vaccination. Vet Microbiol 77(3–4):475–485
Eppleston J, Britton A, Windsor P, Hall D, Whittington R and Jones S (2000) Progress in a field trial to determine the effectiveness of a killed Mycobacterium paratuberculosis vaccine for the control of OJD in Australian sheep flock, Proceedings for Australian Sheep Vetenarian Society 64–67
Eppleston J, Reddacliff LA, Windsor PA, Whittington RJ and Johnes S (2005) Efficacy of killed Mycobacterium paratuberculosis vaccine for the control of OJD in Australian sheep flocks. In Manning EJB, Nielsen SS, editors, Proceedings of the eighth ICP, pp 187–94
Gwozdz JM, Thompson KG, Manktelow BW, Murray A, West DM (2000) Vaccination against paratuberculosis of lambs already infected experimentally with Mycobacterium avium subspecies paratuberculosis. Aust Vet J 78(8):560–566
Juste RA, Garcia-Marin JF, Peris B, de Ocariz CS, Badiola JJ (1994) Experimental infection of vaccinated and non-vaccinated lambs with Mycobacterium paratuberculosis. J Comp Pathol 110(2):185–194
Kalis CH, Hesselink JW, Barkema HW, Collins MT (2001) Use of long term vaccination with a killed vaccine to prevent faecal shedding of Mycobacterium avium subsp paratuberculosis in dairy herd. Am J Vet Res 62(2):270–274
Kumar S, Singh SV, Singh AV, Singh PK, Sohal JS, Maitra A (2010) Wildlife (Boselaphus tragocamelus)- small ruminant (goat and sheep) interface in the transmission of ‘Bison type’ genotype of Mycobacterium avium subspecies paratuberculosis in India. Comp Immunol Microbiol Infect Dis 33(2):145–159
Lambert G (1984) Paratuberculosis: prevalence, diagnosis, prevention and treatment, Proceedings of the 17th Annual Convention of American Association of Bovine Practitioners, Des Moines, Iowa, USA.
Millar DS, Withey SJ, Tizard MLV, Ford JG, Hermon-Taylor J (1995) Solid-phase hybridization capture of low-abundance target DNA sequences: application to the polymerase chain reaction detection of Mycobacterium paratuberculosis and Mycobacterium avium subsp silvaticum. Anal Biochem 226(2):325–330
Ott SL, Wells SJ, Wagner BA (1999) Herd-level economic losses associated with Johne’s disease on US dairy operations. Prev Vet Med 40(3–4):179–192
Perez V, Garcia Marin JF, Bru R, Moreno B, Badiola JJ (1995) Results of vaccination of adult animals against ovine paratuberculosis. Medicina Vet 12(3):196–201
Reddacliff L, Eppleston J, Windsor P, Whittington R, Jones S (2006) Efficacy of killed vaccine for the control of paratuberculosis in Australian Sheep flocks. Vet Microbiol 115:77–90
Saxegaard F, Fodstad FH (1985) Control of paratuberculosis (Johne’s disease) in goats by vaccination. Vet Rec 116(16):439–441
Sevilla I, Singh SV, Garrido JM, Aduriz G, Rodriguez S, Geijo MV, Whittington RJ, Saunders V, Whitlock RH, Juste RA (2005) Molecular typing of Mycobacterium avium subspecies paratuberculosis strains from different hosts and regions. Sci Tech Rev (OIE) 24:1061–1066
Singh SV, Singh PK, Singh AV, Sohal JS, Gupta VK, Vihan VS (2007) Comparative efficacy of an indigenous ‘inactivated vaccine’ using highly pathogenic field strain of Mycobacterium avium subspecies paratuberculosis ‘Bison type’ with a commercial vaccine for the control of Capri-paratuberculosis in India. Vaccine 25:7102–7110
Singh PK, Singh SV, Kumar H, Sohal JS, Singh AV (2010a) Diagnostic application of IS900 PCR using blood as a source sample for the detection of Mycobacterium avium subspecies paratuberculosis in early and sub-clinical cases of Caprine Paratuberculosis. Vet Med Int. doi:10,4061/2010/748621, 8 pages
Singh SV, Singh PK, Singh AV, Sohal JS, Sharma MC (2010b) Therapeutic effects of a new “Indigenous vaccine”developed using novel native “Indian Bison type” Genotype of Mycobacterium avium subspecies paratuberculosis for the control of clinical Johne’s disease in naturally infected goatherds in India. Vet Med Int. doi:10.4061/2010/351846
Singh SV, Singh PK, Singh MK, Singh AV, Sohal JS (2011) Therapeutic potential of Johne’s disease vaccine: a follow up post vaccination study in a goatherd of endangered Jamunapari breed, naturally infected with Mycobacterium avium subsp. Paratuberculosis. Int J Livest Prod 2(13):192–204
Sohal JS, Singh SV, Subhodh S, Singh AV, Singh PK, Sheoran N, Komal S, Narayanasamy K, Maitra A (2007) Mycobacterium avium subspecies paratuberculosis diagnosis and strain typing–present status and future development. Indian J Exp Biol 45:843–852
Sohal JS, Sheoran N, Narayanasamy K, Brahmachari V, Singh SV, Subodh S (2009) Genomic analysis of local isolate of Mycobacterium avium subspecies paratuberculosis. Vet Microbiol 134(3–4):375–382
Srivastav AK (2010). Prophylactic and therapeutic effect of johne’s disease vaccine in cattle. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigya Vishwavidyalya Evam Go Anusandhan Sansthan, Mathura, Uttar Pradesh.
Valle’e H, Rinjard P, Valle’e M (1934) Sur la pr’emunition de l’enterite paratuberculeuse des bovides. Revue G’en’erale De M’edecine V’et’erinaire 43:777–779
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Authors are thankful to Department of Microbiology, College of Veterinary Science and A.H. and Director, CIRG, for extending necessary facilities for the work.
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Singh, K., Chandel, B.S., Chauhan, H.C. et al. Efficacy of ‘indigenous vaccine’ using native ‘Indian bison type’ genotype of Mycobacterium avium subspecies paratuberculosis for the control of clinical Johne’s disease in an organized goat herd. Vet Res Commun 37, 109–114 (2013). https://doi.org/10.1007/s11259-013-9551-4
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DOI: https://doi.org/10.1007/s11259-013-9551-4