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Biological control of Ascaris suum eggs by Pochonia chlamydosporia fungus

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Abstract

Ascaris suum is a gastrointestinal nematode parasite of swines. The aim of this study was to observe Pochonia chlamydosporia fungus on biological control of A. suum eggs after fungus passage through swines gastrointestinal tract. Eighteen pigs, previously dewormed, were randomly divided into three groups: group 1, treated with the fungus isolate VC4; group 2, treated with the fungus isolate VC1 and group 3 did not receive fungus (control). In the treated groups, each animal received a 9 g single dose of mycelium mass containing P. chlamydosporia (VC1 or VC4). Thereafter, animal fecal samples were collected at the following intervals: 8, 12, 24, 36, 48, 72 and 96 h after treatment beginning and these were poured in Petri dishes containing 2% water-agar culture medium. Then, 1,000 A. suum eggs were poured into each dish and kept in an incubator at 26°C and in the dark for 30 days. After this period, approximately 100 eggs were removed from each Petri dish and morphologically analyzed under light microscopy following the ovicidal activity parameters. The higher percentage observed for isolated VC4 eggs destruction was 57.5% (36 h) after fungus administration and for isolate VC1 this percentage was 45.8% (24 h and 72 h) (p > 0.01). P. chlamydosporia remained viable after passing through the gastrointestinal tract of swines, maintaining its ability of destroying A. suum eggs.

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Acknowledgements

CNPq and Capes for financial support.

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Authors and Affiliations

  1. Departamento de Medicina Veterinária, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    Sebastião Rodrigo Ferreira, Jackson Victor de Araújo, Fábio Ribeiro Braga, Juliana Milani Araujo & Luiza Neme Frassy

  2. Departamento de Zootecnia, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil

    Aloízio Soares Ferreira

Authors
  1. Sebastião Rodrigo Ferreira
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  2. Jackson Victor de Araújo
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  3. Fábio Ribeiro Braga
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  4. Juliana Milani Araujo
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  5. Luiza Neme Frassy
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  6. Aloízio Soares Ferreira
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Correspondence to Sebastião Rodrigo Ferreira.

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Jackson Victor de Araújo: CNPq Scholarship

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Ferreira, S.R., de Araújo, J.V., Braga, F.R. et al. Biological control of Ascaris suum eggs by Pochonia chlamydosporia fungus. Vet Res Commun 35, 553–558 (2011). https://doi.org/10.1007/s11259-011-9494-6

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