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Determination of Bovine Serum Low-density Lipoprotein Cholesterol using the N-geneous Method

Miyamoto, T., Sugiyama, Y., Suzuki, J., Oohashi, T. and Takahashi, Y., 2006. Determination of bovine serum low-density lipoprotein cholesterol using the N-geneous method. Veterinary Research Communications, 30(5), 467–474

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Abstract

The N-geneous method is a recently developed method for determination of low-density lipoprotein cholesterol (LDL-C) in human serum. In the present study, we attempted to adapt this method to bovine serum. The values of LDL-C obtained using the N-geneous method were highly correlated with those from the method using ultracentrifugation and heparin sepharose affinity chromatography (r = 0.934, p < 0.001). The reproducibility of this method was acceptable (intra-assay CV 4.2%, inter-assay CV 7.6%) for clinical use. Using the N-geneous method, serum LDL-C was evaluated in cows around parturition, and in cows with fatty liver induced by fasting. The concentration of LDL-C decreased significantly in cows close to parturition. A reduced concentration of LDL-C was also observed in cows with fatty liver. In both cases, the changes of LDL-C were similar to those of apolipoprotein B (apoB)-100, and the values of LDL-C were highly correlated (r = 0.876, p < 0.001) with those of apoB-100. These results suggest that the concentration of LDL-C reflects the level of apoB-100. The N-geneous method is simple and rapid, and might to be a useful tool to elucidate the clinical significance of LDL-C in bovine serum.

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Abbreviations

apoB:

apolipoprotein B

HDL:

high-density lipoprotein

HDL-C:

high-density lipoprotein cholesterol

LDL:

low-density lipoprotein

LDL-C:

low-density lipoprotein cholesterol

NEFA:

non-esterified fatty acid

SRID:

single radial immunodiffusion

TC:

total cholesterol

TG:

triglyceride

VLDL:

very lowdensity lipoprotein

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Authors and Affiliations

  1. Department of Production Disease, National Institute of Animal Health, Kannondai, Tsukuba, Ibaraki

    T. Miyamoto, T. Oohashi & Y. Takahashi

  2. Gifu Livestock Hygiene Service Center, Gifu

    Y. Sugiyama

  3. Mizoguchi Livestock Hygiene Service Center, Mizoguchi, Tottori, Japan

    J. Suzuki

Authors
  1. T. Miyamoto
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  2. Y. Sugiyama
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  3. J. Suzuki
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  4. T. Oohashi
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  5. Y. Takahashi
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Correspondence to T. Miyamoto.

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Miyamoto, T., Sugiyama, Y., Suzuki, J. et al. Determination of Bovine Serum Low-density Lipoprotein Cholesterol using the N-geneous Method. Vet Res Commun 30, 467–474 (2006). https://doi.org/10.1007/s11259-006-3276-6

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  • DOI: https://doi.org/10.1007/s11259-006-3276-6

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