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Tissue and urinary KIM-1 relate to tumor characteristics in patients with clear renal cell carcinoma

  • Urology - Original Paper
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Abstract

The objective of this prospective follow-up trial was to ascertain whether the urinary kidney injury molecule-1 (uKIM-1) associates with tumor tissue (tKIM-1) expression and with the pathological characteristics of clear renal cell carcinoma (cRCC) in radically nephrectomized (RN) and/or in partially nephrectomized (PN) patients with cRCC, pre- and postoperatively. This clinical study included 40 patients subjected to RN/PN (cRCC group) and 30 healthy volunteers (control group). Urinary KIM-1 was determined by ELISA TIM-1/KIM-1 kit and normalized by urinary creatinine. Immunohistochemical staining (monoclonal anti-human anti-TIM-1/KIM-1/HAVCR antibody) was used for semiquantitative analysis of the tKIM-1 expression and expressed as a score (% KIM-1 positively stained tubules). Both markers were interpreted in terms of the tumor characteristics comprising tumor size, Fuhrman grade, pathological (pT) stage, tumor/nodes/metastasis (TNM) stage, lymphovascular invasion and type of surgery RN/PN. Preoperative uKIM-1 was significantly higher in the cRCC group compared to controls, such as uKIM-1 was statistically higher in RN than in PN patients. Postoperatively, uKIM-1 decreased to control values. Expression of tKIM-1 was documented in all nephrectomized patients. Significant associations were achieved between uKIM-1 and tKIM-1 and with considered tumor characteristics, especially with tumor size and grade. Based on the accomplished associations, we found uKIM-1 as a highly sensitive marker for cRCC diagnosis.

The clinical trial registration number: 1110-2012.

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Acknowledgements

This study was supported by Grants from the Ministry of Defense of the Republic of Serbia (MFVMA/3/13-15, MFVMA/24/13-15) and Ministry of Education, Science and Technological Development of the Republic of Serbia (III41018). We are thankful to the reviewers for contributive suggestions and criticisms, helping us make this a much better paper.

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Authors and Affiliations

  1. Faculty of Medicine, Clinic of Nephrology, Military Medical Academy, Belgrade, Serbia

    Mirjana Mijuskovic, Djoko Maksic & Brankica Terzic

  2. Faculty of Medicine, Institute for Medical Research, Military Medical Academy, Belgrade, Serbia

    Ivan Stanojevic & Danilo Vojvodic

  3. Faculty of Medicine, Clinic of Urology, Military Medical Academy, Belgrade, Serbia

    Novak Milovic & Predrag Aleksic

  4. Faculty of Medicine, Institute of Pathology, Military Medical Academy, Belgrade, Serbia

    Snezana Cerovic & Bozidar Kovacevic

  5. Faculty of Medicine, Institute of Medical Biochemistry, Military Medical Academy, Belgrade, Serbia

    Tamara Andjelic

  6. Faculty of Medical Sciences, Clinic of Urology, Nephrology and Dialysis, Clinic Centre of Kragujevac, University of Kragujevac, Kragujevac, Serbia

    Dejan Petrovic

  7. Faculty of Pharmacy, Department of Toxicology, University of Belgrade, Belgrade, Serbia

    Mirjana Djukic

Authors
  1. Mirjana Mijuskovic
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  2. Ivan Stanojevic
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  3. Novak Milovic
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  4. Snezana Cerovic
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  9. Predrag Aleksic
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  10. Brankica Terzic
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Correspondence to Mirjana Mijuskovic.

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Mijuskovic, M., Stanojevic, I., Milovic, N. et al. Tissue and urinary KIM-1 relate to tumor characteristics in patients with clear renal cell carcinoma. Int Urol Nephrol 50, 63–70 (2018). https://doi.org/10.1007/s11255-017-1724-6

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  • DOI: https://doi.org/10.1007/s11255-017-1724-6

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