Skip to main content
SpringerLink
Log in

Effect of different concentrations of resveratrol on nuclear maturation and in-vitro development competence of oocytes of Nili Ravi buffalo

  • Regular Articles
  • Published:
Tropical Animal Health and Production Aims and scope Submit manuscript

Abstract

Buffaloes are considered animals of the future with the ability to survive under unfavorable conditions. However, the lack of access to superior germplasm poses a significant challenge to increasing buffalo production. Resveratrol has been shown to improve oocyte quality and developmental competence in various animals during in vitro embryo development. However, limited information is available on the use of resveratrol to improve the in vitro maturation and development competence of Nili Ravi buffalo oocytes. Therefore, the current study aimed to investigate the influence of different concentrations of resveratrol on the maturation, fertilization, and development of buffalo oocytes under in vitro conditions. Oocytes were collected from ovaries and subjected to in vitro maturation (IVM) using varying concentrations of resveratrol (0 µM, 0.5 µM, 1 µM, 1.5 µM, and 2 µM), and the maturation process was assessed using a fluorescent staining technique. Results indicated no significant differences in oocyte maturation, morula rate, and blastocyst rate among the various resveratrol concentrations. However, the cleavage rate notably increased with 1 µM and 1.5 µM concentrations of resveratrol (p < 0.05). In conclusion, the study suggests that adding 1 µM of resveratrol into the maturation media may enhance the cleavage and blastocyst hatching of oocytes of Nili Ravi buffaloes. These findings hold promise for advancing buffalo genetics, reproductive performance, and overall productivity, offering potential benefits to the dairy industry, especially in Asian countries.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Data availability

Data can be provided on request from the author.

References

  • Agarwal, A., Maldonado Rosas, I., Anagnostopoulou, C., Cannarella, R., Boitrelle, F., Munoz, L.V., Finelli, R., Durairajanayagam, D., Henkel, R. and Saleh, R. (2022). Oxidative stress and assisted reproduction: a comprehensive review of its pathophysiological role and strategies for optimizing embryo culture environment. Antioxidants, 11(3), p.477.

  • Brisard D, Chesnel F, Elis S, Desmarchais A, Sánchez-Lazo L, Chasles M, Maillard V, Uzbekova S. (2014). Tribbles expression in cumulus cells is related to oocyte maturation and fatty acid metabolism. Journal of Ovarian Research. 7(1): 1–13.

    Article  Google Scholar 

  • Ciani F, Maruccio L, Cocchia N, d’Angelo D, Carotenuto D, Avallone L, Namagerdi AA, Tafuri S. (2021). Antioxidants in assisted reproductive technologies: An overview on dog, cat, and horse. Journal of Advanced Veterinary and Animal Research. 8(1): 173.

    Article  PubMed  PubMed Central  Google Scholar 

  • De Matos D, Furnus C. (2000). The importance of having high glutathione (GSH) level after bovine in vitro maturation on embryo development: effect of β-mercaptoethanol, cysteine and cystine. Theriogenology. 53(3): 761–771.

    Article  PubMed  Google Scholar 

  • Espinosa-Diez C, Miguel V, Mennerich D, Kietzmann T, Sánchez-Pérez P, Cadenas S, Lamas S. (2015). Antioxidant responses and cellular adjustments to oxidative stress. Redox Biology. 6: 183–197.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ferré, L. B., Kjelland, M. E., Strøbech, L. B., Hyttel, P., Mermillod, P., & Ross, P. J. (2020). Recent advances in bovine in vitro embryo production: Reproductive biotechnology history and methods. Animal, 14(5), 991–1004.

    Article  PubMed  Google Scholar 

  • García-Martínez T, Vendrell-Flotats M, Martínez-Rodero I, Ordóñez-León EA, Álvarez-Rodríguez M, López-Béjar M, Yeste M, Mogas T. (2020). Glutathione ethyl ester protects in vitro-maturing bovine oocytes against oxidative stress induced by subsequent vitrification/warming. International Journal of Molecular Sciences. 21(20): 7547.

    Article  PubMed  PubMed Central  Google Scholar 

  • Jainudeen M editor. Proceedings, II World Buffalo Congress, New Delhi, India, December 1988. Volume II, Part II. Invited papers and special lectures.; 1990.

  • Jang J-H, Surh Y-J. (2001). Protective effects of resveratrol on hydrogen peroxide-induced apoptosis in rat pheochromocytoma (PC12) cells. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 496(1–2): 181–190.

    Article  CAS  Google Scholar 

  • Jeong BS, Yang X. (2001). Cysteine, glutathione, and Percoll treatments improve porcine oocyte maturation and fertilization in vitro. Molecular Reproduction and Development. 59(3): 330–335.

    Article  CAS  PubMed  Google Scholar 

  • Jeong YW, Park SW, Hossein MS, Kim S, Kim JH, Lee SH, Kang SK, Lee BC, Hwang WS. (2006). Antiapoptotic and embryotrophic effects of α-tocopherol and L-ascorbic acid on porcine embryos derived from in vitro fertilization and somatic cell nuclear transfer. Theriogenology. 66(9): 2104–2112.

    Article  CAS  PubMed  Google Scholar 

  • Kobayashi M, Lee E-S, Fukui Y. (2006). Cysteamine or β-mercaptoethanol added to a defined maturation medium improves blastocyst formation of porcine oocytes after intracytoplasmic sperm injection. Theriogenology. 65(6): 1191–1199.

    Article  CAS  PubMed  Google Scholar 

  • Kwak S-S, Cheong S-A, Jeon Y, Lee E, Choi K-C, Jeung E-B, Hyun S-H. (2012). The effects of resveratrol on porcine oocyte in vitro maturation and subsequent embryonic development after parthenogenetic activation and in vitro fertilization. Theriogenology. 78(1): 86–101.

    Article  CAS  PubMed  Google Scholar 

  • Labarrere CA, Kassab GS. (2022). Glutathione: A Samsonian life-sustaining small molecule that protects against oxidative stress, ageing and damaging inflammation. Frontiers in Nutrition. 9: 2653.

    Article  Google Scholar 

  • Lee S, Park EJ, Moon JH, Kim SJ, Song K, Lee BC. (2015). Sequential treatment with resveratrol-trolox improves development of porcine embryos derived from parthenogenetic activation and somatic cell nuclear transfer. Theriogenology. 84(1): 145–154.

    Article  CAS  PubMed  Google Scholar 

  • Li, Y., Wang, J., Zhang, Z., et al. (2016). Resveratrol compares with melatonin in improving in vitro porcine oocyte maturation under heat stress. Journal of Animal Science and Biotechnology, 7, 33. Liu, E.-J., Sun, A.-G., Zhao, S.-G., Liu, H., Ma, S.-Y., Li, M., Huai, Y.-X., Zhao, H., & Liu, H.-B. (2018). Resveratrol improves in vitro maturation of oocytes in aged mice and humans. Fertility and Sterility, 109(5), 900–907.

  • Lonergan P, Monaghan P, Rizos D, Boland M, Gordon I. (1994). Effect of follicle size on bovine oocyte quality and developmental competence following maturation, fertilization, and culture in vitro. Molecular Reproduction and Development. 37(1): 48–53.

    Article  CAS  PubMed  Google Scholar 

  • Martín-Maestro, A., Sánchez-Ajofrín, I., Maside, C., Peris-Frau, P., Medina-Chávez, D.A., Cardoso, B., Navarro, J.C., Fernández-Santos, M.R., Garde, J.J. and Soler, A.J. (2020). Cellular and molecular events that occur in the oocyte during prolonged ovarian storage in sheep. Animals, 10(12), p.2414.

  • Meng Q, Li J, Wang C, Shan A. (2023). Biological function of resveratrol and its application in animal production: a review. Journal of Animal Science and Biotechnology. 14(1): 1–23.

    Article  Google Scholar 

  • Mukherjee A, Kumar D, Singh K, Chauhan M, Singla S, Palta P, Manik R. (2010). Assessment of DNA damage during in vitro development of buffalo (Bubalus bubalis) embryos: effect of cysteamine. Reproduction in Domestic Animals. 45(6): 1118–1121.

    Article  CAS  PubMed  Google Scholar 

  • Mukherjee A, Malik H, Saha AP, Dubey A, Singhal DK, Boateng S, Saugandhika S, Kumar S, De S, Guha SK. (2014). Resveratrol treatment during goat oocytes maturation enhances developmental competence of parthenogenetic and hand-made cloned blastocysts by modulating intracellular glutathione level and embryonic gene expression. Journal of Assisted Reproduction and Genetics. 31(2): 229–239.

    Article  PubMed  Google Scholar 

  • Neglia G, de Nicola D, Esposito L, Salzano A, D’Occhio MJ, Fatone G. (2020). Reproductive management in buffalo by artificial insemination. Theriogenology. 150: 166–172.

    Article  CAS  PubMed  Google Scholar 

  • Nikseresht M, Toori MA, Rahimi HR, Fallahzadeh AR, Kahshani IR, Hashemi SF, Bahrami S, Mahmoudi R. (2017). Effect of antioxidants (β-mercaptoethanol and cysteamine) on assisted reproductive technology in vitro. Journal of Clinical and Diagnostic Research: JCDR. 11(2): BC10.

    CAS  PubMed  PubMed Central  Google Scholar 

  • Okamoto, N., Sato, Y., Kawagoe, Y., Shimizu, T. and Kawamura, K. (2022). Short-term resveratrol treatment restored the quality of oocytes in aging mice. Aging (Albany NY), 14(14), p.5628.

  • Parrish JJ. 2014. Bovine in vitro fertilization: in vitro oocyte maturation and sperm capacitation with heparin. Theriogenology. 81(1): 67–73.

    Article  PubMed  Google Scholar 

  • Pasquariello, R., Verdile, N., Brevini, T. A., Gandolfi, F., Boiti, C., Zerani, M., & Maranesi, M. (2020). The role of resveratrol in mammalian reproduction. Molecules, 25(19), 4554.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Pervaiz S, Holme AL. (2009). Resveratrol: its biologic targets and functional activity. Antioxidants & Redox Signaling. 11(11): 2851–2897.

    Article  CAS  Google Scholar 

  • Pohler, K.G., Franco, G.A., Reese, S.T. and Smith, M.F. (2020). Physiology and pregnancy of beef cattle. In Animal Agriculture (pp. 37–55). Academic Press.

  • Rodriguez-Martinez, H. (2012). Assisted reproductive techniques for cattle breeding in developing countries: A critical appraisal of their value and limitations. Reproduction in Domestic Animals, 47(1), 21–26.

    Article  PubMed  Google Scholar 

  • Sabapara G, Padheriya Y, Kharadi V. (2016). A field survey of feeding and breeding practices at peri urban buffalo farms of Surat city of Gujarat. Journal of Animal Research. 6(5): 933–939.

    Article  Google Scholar 

  • Siddiky, M. N., & Faruque, M. O. (2017). Buffaloes for dairying in South Asia: potential, challenges and way forward. SAARC Journal of Agriculture, 15(2), 227–239.

    Article  Google Scholar 

  • Sovernigo, T. C., Adona, P. R., Monzani, P. S., Guemra, S., Barros, F. D. A., Lopes, F. G., & Leal, C. L. V. (2017). Effects of supplementation of medium with different antioxidants during in vitro maturation of bovine oocytes on subsequent embryo production. Reproduction in Domestic Animals, 52(4), 561–569.

    Article  CAS  PubMed  Google Scholar 

  • Uhm SJ, Gupta MK, Das ZC, Lim KT, Yang JH, Lee HT. (2011). Effect of 3-hydroxyflavone on pig embryos produced by parthenogenesis or somatic cell nuclear transfer. Reproductive Toxicology. 31(2): 231–238.

    Article  CAS  PubMed  Google Scholar 

  • Velilla E, López-Béjar M, Rodríguez-González E, Vidal F, Paramio M-T. (2002). Effect of Hoechst 33342 staining on developmental competence of prepubertal goat oocytes. Zygote. 10(3): 201–208.

    Article  PubMed  Google Scholar 

  • Villanueva M, Mingala C, Tubalinal G, Gaban P, Nakajima C, Suzuki Y. Emerging Infectious Diseases in Water Buffalo: An Economic and Public Health Concern; IntechOpen: London, UK, (2018). Emerging Infectious Diseases in Water Buffalo: An Economic and Public Health Concern.

  • Wang F, Tian X, Zhang L, He C, Ji P, Li Y, Tan D, Liu G. (2014). Beneficial effect of resveratrol on bovine oocyte maturation and subsequent embryonic development after in vitro fertilization. Fertility and Sterility. 101(2): 577–586. e571.

    Article  CAS  PubMed  Google Scholar 

  • Wang, Y., Zhang, M., Chen, Z. J., & Du, Y. (2018). Resveratrol promotes the embryonic development of vitrified mouse oocytes after in vitro fertilization. In Vitro Cellular & Developmental Biology-Animal, 54, 430–438.

    Article  CAS  Google Scholar 

  • Warriach H, McGill D, Bush R, Wynn P, Chohan K. (2015). A review of recent developments in buffalo reproduction—a review. Asian-Australasian Journal of Animal Sciences. 28(3): 451.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • You J, Kim J, Lim J, Lee E. (2010). Anthocyanin stimulates in vitro development of cloned pig embryos by increasing the intracellular glutathione level and inhibiting reactive oxygen species. Theriogenology. 74(5): 777–785.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

All authors thanks to Ms. Syeda Anum Masood Bokhari MNS University of Agriculture, Multan for statistical analysis on collected data.

Funding

The current study was funded by Technology Development Funds (TDF), Higher Education Commission (HEC) under TDF-02-059 research project.

Author information

Authors and Affiliations

  1. Faculty of Veterinary and Animal Sciences, Muhammad Nawaz Shareef University of Agriculture, Multan, 66000, Pakistan

    Samar Wafa Kabeer, Aziz Ul-Rahman, Rana Muhammad Shahbakht, Ahsan Anjum, Hafeez ur Rehman Ali Khera, Ali Haider, Faisal Riaz, Naheed Bano, Muhammad Asif Raza & Junaid Ali Khan

  2. Department of Theriogenology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan

    Amjad Riaz, Muhammad Yaseen & Muhammad Saleem

  3. Department of Zoology, University of Education, D.G Khan Campus, Dera Ghazi Khan, Pakistan

    Riffat Yasin

Authors
  1. Samar Wafa Kabeer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Amjad Riaz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Aziz Ul-Rahman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rana Muhammad Shahbakht
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ahsan Anjum
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hafeez ur Rehman Ali Khera
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ali Haider
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Faisal Riaz
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Riffat Yasin
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Muhammad Yaseen
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Muhammad Saleem
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Naheed Bano
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Muhammad Asif Raza
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Junaid Ali Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Kabeer SW, Riaz A and Ul-Rahman A conceived and designed the research. Kabeer SW, Ul-Rahman A, Yaseen M, Shahbakht RM, Haider A, and Saleem M, conducted experiments. Kabeer SW, Ul-Rahman A, Khera HRA, Yasin R, and Bano N, did data analysis. Kabeer SW, Ul-Rahman A, Riaz A, Raza MA, and Khan JA wrote and reviewed the draft. All the authors worked in a coordinated way during reading and preparing this manuscript.

Corresponding authors

Correspondence to Samar Wafa Kabeer or Aziz Ul-Rahman.

Ethics declarations

Ethical approval

Not applicable.

Consent to participate

Not applicable.

Conflict of interest

All authors declared no conflict of interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kabeer, S.W., Riaz, A., Ul-Rahman, A. et al. Effect of different concentrations of resveratrol on nuclear maturation and in-vitro development competence of oocytes of Nili Ravi buffalo. Trop Anim Health Prod 56, 105 (2024). https://doi.org/10.1007/s11250-024-03952-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11250-024-03952-w

Keywords

Search

Navigation