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Physicochemical and microbiological parameters of rumen fluid of lactating goats fed diets containing crude glycerin from waste frying oil used in biodiesel production

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Abstract

Crude glycerin from the biodiesel industry is a alternative energy source used to replace part of the energy components of the diet, since the nutritional value of glycerol makes it suitable for use in ruminant diets. The objective was to analyze the effects including crude glycerin (CG) derived from the production of biodiesel from residual frying oil, in diets of lactating goats on the physicochemical and microbiological parameters of rumen fluid. Eight 2-year-old goats weighting approximately 42.06 ± 3.5 kg of body weight (BW) were kept in individual stalls, receiving diets containing 0, 7, 14 and 21% of CG (% DM). The experiment was carried out according to a double 4 × 4 Latin square and lasted for 80 days. Orthogonal contrasts were tested to determine linear and quadratic effects, as well as the effects of 0% CG in the diet in comparison to the other treatments. There was a linear decreasing effect on DM intake (P = 0.001) and neutral detergent fiber (P = 0.0001), as well as a quadratic effect (P = 0.0002) on ether extract (EE) intake with increasing level of CG in the diet. Including CG affected motility and density of the protozoa (P = 0.0001). Treatments had no effect on pH, N-NH3 concentration and percentage of protozoa in the rumen liquid. There was increasing linear effect on sedimentation and flotation time (P = 0.001). Therefore, including 14 and 21% CG in the diet as a source of glycerol decreased nutrient intake and altered the ruminal microbiota, but pH and N-NH3 were not affected. Thus, to avoid impairment to the physicochemical and microbiological parameters of the rumen liquid of goats, the inclusion of CG should not exceed 7% DM.

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References

  • Almeida, V.V.S., Silva, R.R., Queiroz, A.C., Oliveira, A.C Silva, F.F., Abreu Filho, G., Lisboa, M.M., Souza, S.O., 2014. Economic viability of the use of crude glycerin supplements in diets for grazing crossbred calves. Revista Brasileira de Zootecnia, 43,382-389. https://doi.org/10.1590/S1516-35982014000700007.

    Article  Google Scholar 

  • Association of Official Analytical Chemists – AOAC. (1990). Official Methods of Analysis: 15ª ed. AOAC, Washington, p. 69-88.

    Google Scholar 

  • Association of Official Analytical Chemists – AOAC. (2007). Official Methods of Analysis: 18ª ed. AOAC, Gaithersburg, p.40–44.

  • Barros, M.C.C., Marques, J.A., Silva, F.F., Silva, R. R., Guimarães, G.S., Silva, L.L. Araújo, F.L., 2015. Glicerina bruta na dieta de ovinos confinados: consumo, digestibilidade, desempenho, medidas morfométricas da carcaça e características da carne. Semina: Ciências Agrárias, 36(1), 453–466. https://doi.org/10.5433/1679-0359.2015v36n1p453.

  • Beserra, V.A., Cesar, A.S., Peres, A.A.C., 2016. Adoção da glicerina bruta na dieta animal e seu impacto no produto final. Archivos de zootecnia, 65(250), 259-265.

    Article  CAS  Google Scholar 

  • Borges, N.C., Orsine, G.F., Silva, L.A.F., Bernardes, K.M., Martins, M.E.P., Fioravanti, M.C.S., 2011. Parâmetros físico-químicos e microbiológicos do fluido ruminal de ovinos confinados submetidos a crescentes níveis de mistura mineral energético-protéica. Ciência animal brasileira, 12(3), 392-399.

    Article  Google Scholar 

  • Corradini, S.A.S., Madrona, G.S., Visentainer, J.V., Bonafe, E.G., Carvalho, C.B., Roche, P.M., Prado, I.N., 2014. Sensorial and fatty acid profileof ice cream manufactured with milk of crossbred cows fed with palm oil and coconut fat. Journal of Dairy Science, 97, 1-9. https://doi.org/10.3168/jds.2014-8290.

    Article  CAS  Google Scholar 

  • Cunha, C.M., Fernandes, A.R.M., Ricardo, H.A., Cornélio, T.C., Alves, L.G.C., 2014. Glicerina bruta na alimentação de ruminantes. Enciclopédia Biosfera, 10(18), 1872-1888.

    Google Scholar 

  • Dehority, B.A., 1984. Evaluation of sub-sampling and fixation procedures used for counting rumen protozoa. Applied and environmental microbiology, 48(1), 182-185. 0099-2240/84/070182-04$02.00/0

  • Detmann, E. Queiroz, A.C., Cabral L.S., 2012. Métodos para análise de alimentos - INCT - Ciência Animal. 1a ed. Suprema, Visconde do Rio Branco MG, p.193–204.

  • Fávaro, V.R., Ezequiel, J.M.B., D'aurea, A.P., Sancanari, J.B.D., Homem Júnior, A.C., Van Cleef, E.H.C.B., 2014. A utilização de glicerina em dietas para bovinos altera a microbiota ruminal. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 66(5), 1504-1512.

    Article  Google Scholar 

  • Hall, M.B. (2000). Neutral detergent-soluble carbohydrates. Nutritional relevance and analysis. bulletin 339. Gainesville, University of Florida, p.3–5.

  • Khotsakdee, J., Vasupen, K., Wongsuthavas, S., Bureenok, S., Alhaidary, A., Mohamed, H.E., Beynen A.C., Yuangklang, C., 2010. Rumen fermentation and nutriente digestibility in goats fed a tallow-rich ration fortifild with yeast. Research Journal of Biological Sciences, 5(3), 246-250.

    Article  Google Scholar 

  • Kozloski, G.V., 2009. Bioquímica dos Ruminantes. 2a ed. UFSM, Santa Maria, p.25–35.

  • Martinele I., Eifert, E.C., Lana, R.P., Arcuri, P.B., D'Agosto, M., 2008. Efeito da monensina e do óleo de soja sobre os protozoários ciliados do rúmen e correlação dos protozoários com parâmetros da fermentação ruminal e digestivos. Revista Brasileira de Zootecnia, 37(6), 1129-1136. https://doi.org/10.1590/S1516-35982008000600025.

    Article  Google Scholar 

  • Menezes, D.R., Costa, R.G., Araújo, G.G.L., Pereira, L.G.R., Oliveira, P.T.L., Silva, A.E.V.N., Voltolini, T.V., Moraes, S.A., 2012. Parâmetros sanguíneos, hepáticos e ruminais de ovinos alimentados com dietas com farelo de mamona destoxificado. Pesquisa Agropecuária Brasileira, 47(1), 103-110. https://doi.org/10.1590/S0100-204X2012000100014.

    Article  Google Scholar 

  • Messana, J.D., Berchielli, T.T., Arcuri, P.B., Reis, R.A., Canesin, R.C., Ribeiro, A.F., Fiorentini, G., Fernandes, J.J.R., 2013. Rumen fermentation and rumen microbes in Nellore steers receiving diets with different lipid contentes. Revista Brasileira de Zootecnia, 42(3), 204–212. 2013. https://doi.org/10.1590/S1516-35982013000300008.

  • National Research Council - NRC. (2007).Nutrient requirements of small ruminants. National Academy Press, Washington, D.C. p271-299.

    Google Scholar 

  • Radostits, O.M., Mayhew, I.G.J., Houston, D.M., 2002. Exame clínico e diagnóstico em veterinária. 1a. ed. Guanabara Koogan, Rio de Janeiro, p. 332–338.

    Google Scholar 

  • Rodrigues, F.V., Rondina, D., 2013. Alternativas de uso de subprodutos da cadeia do biodiesel na alimentação de ruminantes: glicerina bruta. Acta Veterinaria Brasilica, 7(2), 91-99. https://doi.org/10.21708/avb.2013.7.2.2801.

    Article  Google Scholar 

  • Rosenberger, G., 1993. Exame clínico de bovinos. 3a ed. Guanabara Koogan, Rio de Janeiro, p.44-80.

    Google Scholar 

  • Saleem, A.M., Zanouny, A.I., Singar, A.M., 2018. Effect of glycerol supplementation during early lactation on milk yield, milk composition, nutrient digestibility and blood metabolites of dairy buffaloes, Animal, 12(4), 757-763. doi.org/https://doi.org/10.1017/S175173111700180X.

    Article  CAS  PubMed  Google Scholar 

  • Santos, C.B., Araújo, M.J., Bezerra, L.R., Marques, C.A.T., Torreão, J.N.C., Freitas, N.E., Oliveira Neto, C.B., 2018. Parâmetros hematológicos e bioquímicos de cabras lactantes alimentadas com dietas contendo glicerina bruta oriunda da produção de biodiesel proveniente de óleo de fritura. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 70(6), 1867-1876. https://doi.org/10.1590/1678-4162-10022.

    Article  Google Scholar 

  • Satter, L.D., Slyter, L.L., 1974. Effect of ammonia concentration on rumen microbial production in vitro. British Journal of Nutrition, 32(2), 199-208. https://doi.org/10.1079/BJN19740073.

    Article  CAS  PubMed  Google Scholar 

  • Silva, D.A.V., Ezequiel, J.M.B., Van Cleef, E.H.C.B. 2012. Efeito da glicerina sobre parâmetros físicos e químicos do líquido ruminal e da urina de bovinos de corte. ARS Veterinária, 28(2), 128-133.

    CAS  Google Scholar 

  • Sniffen, C.J., O'Connor, J.D., Van Soest, P.J., Fox, D.G., Russell, J.B., 1992. A net carbohydrate and protein system for evaluating cattle diets: II. Carbohydrate and protein availability. Journal of Animal Science. 70 (12), 3562–3577. 1992. https://doi.org/10.2527/1992.70113562x.

  • Thoh, D., Pakdeechanuan, P., Chanjula, P., 2017. Effect of supplementary glycerin on milk composition and heat stability in dairy goats Animal Bioscience, 30(12), 1711-1717. doi.org/https://doi.org/10.5713/ajas.17.0066.

    Article  CAS  Google Scholar 

  • Van Cleef, E.H.C.B., Almeida, M.T.C., Perez, H.L. Van Cleef, F.O.S., Silva, D.A.V., Ezequiel, J.M.B. 2015. Crude glycerin changes ruminal parameters, in vitro greenhouse gas profile, and bacterial fractions of beef cattle. Livestock Science, 178, 158-164. https://doi.org/https://doi.org/10.1016/j.livsci.2015.06.016.

    Article  Google Scholar 

  • Van Cleef, E.H.C.B., Sancanari, J.B.D., Silva, Z.F., D’Aurea, A.P., Fávaro, V.R., Van Cleef F.O.S., Homem Júnior, A.C., Ezequiel, J.M.B., 2016. High concentrations of crude glycerin on ruminal parameters, microbial yield, and in vitro greenhouse gases production in dairy cows. Canadian Journal of Animal Science, 96, 461-465. doi.org/https://doi.org/10.1139/cjas-2015-0170

    Article  CAS  Google Scholar 

  • Van Soest, P.J., Robertson, J.B., Lewis, B.A., 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74 (10), 3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2.

    Article  PubMed  Google Scholar 

  • Van Soest, P.J., 1994. Nutritional ecology of the ruminant. 2a ed. Cornel University Press, Ithaca, 476. p.253–280.

Download references

Acknowledgements

Graduate Program in Animal Science of the Federal University of Piauí (UFPI), together with the Federal University of Campina Grande and the Federal University of Rio Grande do Norte.

Funding

This work was supported by the National Council for Scientific and Technological Development-CNPq and Coordination for the Improvement of Higher Education Personnel-Capes (Financing Code 001).

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Authors and Affiliations

  1. Universidade Federal Do Piauí, Campus Professora Cinobelina Elvas, BR 135, Avenida Manoel Gracindo Km 1, Planalto Horizonte, Bom Jesus, Piauí, PI 64900-000, Brazil

    Marcos Jácome Araújo, José Pires de Carvalho Neto, Carlos Barbosa Santos, Natylane Eufransino Freitas, Antonio Leandro Chaves Gurgel, Tairon Pannunzio Dias-Silva & Ricardo Loiola Edvan

  2. Universidade Federal Do Rio Grande Do Norte, Km 03 – Distrito de Jundiaí, Macaíba, Rio Grande Do Norte, 59280-000, Brazil

    Carlo Aldrovandi Torreão Marques & Jacira Neves da Costa Torreão

  3. Faculdade de Medicina Veterinára e Zootecnia, Universidade Federal Do Mato Grosso Do Sul, Avenida Senador Filinto Muller, 2443, Vila Ipiranga, Campo Grande, 79070-900, Brazil

    Luis Carlos Vinhas Itavo

  4. Universidade Federal de Campina Grande (UFCG), Avenida Universitária, S/N - Santa Cecília, Patos, Paraíba, 58708-110, Brazil

    Leilson Rocha Bezerra

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  1. Marcos Jácome Araújo
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Contributions

MJdA, JPdCN, CBS, CATM, and JNdCT, conceived and designed research; MJdA, JPdCN, CBS, CATM, JNdCT, NEF, ALCG, and TPDS wrote the manuscript; MJdA, and LRB data analysis and discussion of results; ALCG, TPDS, LCVI and RLE; reviewed the manuscript. MJdA, JPdCN, CBS, CATM, JNdCT, NEF, ALCG, and TPDS conducted experiment, and statistical analysis. All authors read and approved the manuscript.

Corresponding author

Correspondence to Antonio Leandro Chaves Gurgel.

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All procedures were approved by the Ethics Committee on Animal Use of the Federal University of Piauí (CEUA/UFPI), under protocol number 016/14.

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Araújo, M.J., de Carvalho Neto, J.P., Santos, C.B. et al. Physicochemical and microbiological parameters of rumen fluid of lactating goats fed diets containing crude glycerin from waste frying oil used in biodiesel production. Trop Anim Health Prod 55, 295 (2023). https://doi.org/10.1007/s11250-023-03721-1

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