Abstract
In this study, the association between PAPPA2 coding variants and gastrointestinal (GI) nematode fecal egg count (FEC) score in adult Turkish sheep was investigated. For this purpose, the FEC score was determined in adult sheep from six breeds: Karacabey Merino (n = 137), Kivircik (n = 116), Cine capari (n = 109), Karakacan (n = 102), Imroz (n = 73), and Chios (n = 50). Sheep were classified as shedders or non-shedders within breeds and flocks. The first group was the fecal egg shedders (> 50 per gram of feces), and the second group was the no fecal egg shedders (≤ 50 per gram of feces). The exon 1, exon 2, exon 5, exon 7, and a part of 5′UTR of the ovine PAPPA2 gene were genotyped by Sanger sequencing of these two groups. Fourteen synonymous and three non-synonymous single-nucleotide polymorphisms (SNPs) were found. The non-synonymous SNPs, D109N, D391H, and L409R variants, are reported for the first time. Two haplotype blocks were constructed on exon 2 and exon 7. The specific haplotype, C391G424G449T473C515A542 on the exon 2 that carries the 391H variant, was tested against four other common haplotypes. Our results indicate that C391G424G449T473C515A542 haplotype was significantly associated with fecal egg shedding status in adult Turkish sheep (p-value, 0.044).
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Data availability
The data that support the findings of this study are available upon reasonable request from the corresponding author.
All animal procedures in the study were reviewed and approved by the ethics committee of Sheep Breeding and Research Institute (approval number: 1282412), and the authors complied with the ARRIVE guidelines.
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Acknowledgements
We would like to thank the Ministry of Agriculture and Forestry of the Republic of Turkey, the General Directorate of Agricultural Research and Policies, and the Sheep Breeding and Research Institute in Bandirma, Balikesir/Turkey.
Funding
This research has been funded by the Republic of Turkey Ministry of Agriculture and Forestry, General Directorate of Agricultural Research and Policies (Project number: TAGEM/HAYSÜD/15/A01/P02/02–02).
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Y.Y. conceived and designed the study, S.S., R.A., M.K., and V.B. conducted genetics laboratory works, Y.Y., A.T.Ö., S.S., and R.A. performed field sampling, S.K. and O.Y. prepared and arranged the genotypic and phenotypic data, M.Ö. and B.Ş. conducted the parasitological experiments, and C.Ü. reviewed the manuscript and assisted in data interpretation. All authors read and approved the manuscript.
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Yaman, Y., Bay, V., Sevim, S. et al. Ovine PAPPA2 gene coding variants are linked to decreased fecal egg shedding in native Turkish sheep naturally infected with gastrointestinal nematodes. Trop Anim Health Prod 55, 186 (2023). https://doi.org/10.1007/s11250-023-03612-5
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DOI: https://doi.org/10.1007/s11250-023-03612-5