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Hematological parameters, antioxidant status, and gene expression of γ-INF and IL-1β in vaccinated lambs fed different type of lipids

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Abstract

This study was aimed to evaluate the effects of vegetable oils as calcium salt on immune responses and the expression of immune-related genes in vaccinated lambs. Twenty-four lambs (35 kg body weight, 6 months old) were assigned to four treatments with six replicates in a completely randomized design for 40 days. Four concentrates were formulated in which the calcium salts of palm oil, canola oil, corn oil, and flaxseed oil were used. On day 30 of the experiment, lambs were vaccinated by a dose of foot-and-mouth disease virus. The blood samples were collected from jugular vein 10 days after vaccination. The level of malondialdehyde and the activity of liver enzymes were the highest in lambs receiving corn oil and the lowest in lambs receiving flaxseed oil. The highest lymphocytes and the lowest neutrophil percentages were observed in lambs receiving flaxseed oil. There was a significant difference among treatments for the relative genes expression. Flaxseed oil significantly upregulated interferon-γ and corn oil upregulated interleukin-1β. The highest titer against foot-and-mouth disease virus was related to lambs receiving flaxseed oil, and the lowest titer was related to lambs that received corn oil. Flaxseed oil had more beneficial effects on immune response than other oils.

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Data availability

All data analyzed during the current study are available from the corresponding author on request.

References

  • AOAC, (2005). Official Methods of Analysis. 18th ed. Association of Official Analytical Chemists, Washington, DC. USA.

  • Aminoroaya, K., Sadeghi, A. A., Ansari, P. Z., & Kashan, N. (2016). The effects of cyclical higher incubation temperatures on body and organs weights, thyroid hormones and HSP70 gene expression of newly hatched broiler chicks. Kafkas Universitesi Veteriner Fakultesi Dergisi, 22(4), 613-618.

    Google Scholar 

  • Arfuso F, Minuti A, Liotta L, Giannetto C, Trevisi E, Piccione G. & Lopreiato V. (2023). Stress and inflammatory response of cows and their calves during peripartum and early neonatal period. Theriogenology 196:157-166.

    Article  CAS  PubMed  Google Scholar 

  • Armato L., Gianesella M., Morgante M., Fiore E., Rizzo M., Giudice E. & Piccione G. (2016). Rumen volatile fatty acids × dietary supplementation with live yeast and yeast cell wall in feedlot beef cattle, Acta Agriculturae Scandinavica, Section A- Animal Science, 66(2), 119-124.

    CAS  Google Scholar 

  • Ayala, A., Muñoz, M. F., & Argüelles, S. (2014). Lipid peroxidation: production, metabolism, and signaling mechanisms of malondialdehyde and 4-hydroxy-2-nonenal. Oxidative Medicine and Cellular Longevity 2014: 360438.

    Article  PubMed  PubMed Central  Google Scholar 

  • Asgari Safdar, A. H., Sadeghi, A. A., & Chamani, M. (2017). Effects of different fat sources (saturated and unsaturated) on reproductive performance and biological indices of ewes during flushing period. Tropical Animal Health and Production, 49(7), 1447-1453.

    Article  PubMed  Google Scholar 

  • Bertoni, G., Minuti, A., & Trevisi, E. (2015). Immune system, inflammation and nutrition in dairy cattle. Animal Production Science, 55(7), 943-948.

  • Benize, I. F. F., & Strain, J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant” power: the FRAP assay. Anal Biochem, 239, 70-76.

    Article  Google Scholar 

  • Block, E., Kilmer, L.H. & Muller, L.D. (1981). Acid insoluble ash as a marker of digestibility for sheep fed corn plants or hay and for lactating dairy cattle fed hay. Journal of Animal Science, 52, 1164-1169.

    Article  CAS  PubMed  Google Scholar 

  • Calder P.C. (1996). Dietary modification of inflammation with lipids. Lipids, 31, S23–S31.

    Google Scholar 

  • Calder P.C. (2010). Omega-3 fatty acids and inflammatory processes. Nutrients, 2(3), 355-74.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Calder P.C. (2013). Omega-3 polyunsaturated fatty acids and inflammatory processes: nutrition or pharmacology? British Journal of Clinic and Pharmacology, 75, 645-662.

    Article  CAS  Google Scholar 

  • Chen, L., Deng, H., Cui, H., Fang, J., Zuo, Z., Deng, J.,Wang, X. & Zhao, L.(2018). Inflammatory responses and inflammation-associated diseases in organs. Oncotarget 9(6), 7204-7218.

    Article  PubMed  Google Scholar 

  • Chen, F., Castranova, V., Shi, X., & Demers, L. M. (1999). New insights into the role of nuclear factor-κB, a ubiquitous transcription factor in the initiation of diseases. Clinical Chemistry, 45(1), 7-17.

    Article  CAS  PubMed  Google Scholar 

  • Chikara, S., Mamidi, S., Sreedasyam, A., Chittem, K., Pietrofesa, R., Zuppa, A. & Reindl, K. M. (2018). Flaxseed consumption inhibits chemically induced lung tumorigenesis and modulates expression of phase II enzymes and inflammatory cytokines in A/J miceflaxseed inhibits chemically induced lung tumorigenesis. Cancer Prevention Research, 11(1), 27-37.

    Article  CAS  PubMed  Google Scholar 

  • Christman, J. W., Lancaster, L. H., & Blackwell, T. S. (1998). Nuclear factor k B: a pivotal role in the systemic inflammatory response syndrome and new target for therapy. Intensive Care Medicine, 24(11), 1131.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Coleman, D. N., Rivera-Acevedo, K. C., & Relling, A. E. (2018). Prepartum fatty acid supplementation in sheep I. Eicosapentaenoic and docosahexaenoic acid supplementation do not modify ewe and lamb metabolic status and performance through weaning. Journal of Animal Science, 96(1), 364-374.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Darios, F., & Davletov, B. (2006). Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3. Nature, 440(7085), 813-817.

    Article  CAS  PubMed  Google Scholar 

  • Draper, H. H., & Hadley, M. (1990). Oxygen radicals in biological systems part B: oxygen radical antioxidants. Methods in Enzymology, 186, 421-431.

  • Elimam, H., & Ramadan, B. K. (2018). Comparative study of the possible prophylactic and curative effects of flaxseed oil on the lipid profile and antioxidant status of hyperlipidaemic rats. Journal of Applied Pharmacology, 10(1), 257.

    Google Scholar 

  • Francisco, A.E., Santos-Silva, J.M., Portugal, A.P., Alves, S.P., Bessa, R.J. (2019). Relationship between rumen ciliate protozoa and biohydrogenation fatty acid profile in rumen and meat of lambs. PLoS One 14, e0221996. doi: https://doi.org/10.1371/journal.pone.0221996.

  • Gao, L., Wang, J., Sekhar, K. R., Yin, H., Yared, N. F., Schneider, S. N. & Freeman, M. L. (2007). Novel n-3 fatty acid oxidation products activate Nrf2 by destabilizing the association between Keap1 and Cullin3. Journal of Biological Chemistry, 282(4), 2529-2537.

    Article  CAS  PubMed  Google Scholar 

  • Goyal A., Sharma, V., Upadhyay, N., Gill, S., Sihag, M. (2014). Flax and flaxseed oil: an ancient medicine & modern functional food. Journal of Food Science and Technology, 51(9), 1633–1653.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gómez-Cortés, P., Gallardo, B., Mantecón, A. R., Juárez, M., De La Fuente, M. A., & Manso, T. (2014). Effects of different sources of fat (calcium soap of palm oil vs. extruded flaxseed) in lactating ewes’ diet on the fatty acid profile of their suckling lambs. Meat Science, 96(3), 1304-1312.

    Article  PubMed  Google Scholar 

  • Gulliver, C. E., Friend, M. A., King, B. J., & Clayton, E. H. (2012). The role of omega-3 polyunsaturated fatty acids in reproduction of sheep and cattle. Animal Reproduction Science, 131(1-2), 9-22.

    Article  CAS  PubMed  Google Scholar 

  • Hales, K. E., Foote, A. P., Brown-Brandl, T. M., & Freetly, H. C. (2017). The effects of feeding increasing concentrations of corn oil on energy metabolism and nutrient balance in finishing beef steers. Journal of Animal Science, 95(2), 939-948.

    CAS  PubMed  Google Scholar 

  • Han, H., Yan, P., Chen, L., Luo, C., Gao, H., Deng, Q. & Liu, L. (2015). Flaxseed oil containing α-linolenic acid ester of plant sterol improved atherosclerosis in apoE deficient mice. Oxidative Medicine and Cellular Longevity, 2015: 958217.

    Article  PubMed  PubMed Central  Google Scholar 

  • Heshmati, J., Morvaridzadeh, M., Maroufizadeh, S., Akbari, A., Yavari, M., Amirinejad, A. & Sepidarkish, M. (2019). Omega-3 fatty acids supplementation and oxidative stress parameters: a systematic review and meta-analysis of clinical trials. Pharmacological research, 149, 104462.

    Article  CAS  PubMed  Google Scholar 

  • Hiller B. (2014). Recent developments in lipid metabolism in ruminants – the role of fat in maintaining animal health and performance. Animal Production Science, 54(10), 1549-1560.

    Article  CAS  Google Scholar 

  • Iranian Council of Animal Care. (1995). Guide to the care and use of experimental animals, vol. 1. Isfahan University of Technology. Isfahan, Iran.

    Google Scholar 

  • Jump, D. B., Botolin, D., Wang, Y., Xu, J., Christian, B., & Demeure, O. (2005). Fatty acid regulation of hepatic gene transcription. The Journal of Nutrition, 135(11), 2503-2506.

    Article  CAS  PubMed  Google Scholar 

  • Kany, S., Vollrath, J. T., & Relja, B. (2019). Cytokines in inflammatory disease. International Journal of Molecular Sciences, 20(23), 6008.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kliewer, S. A., Lenhard, J. M., Willson, T. M., Patel, I., Morris, D. C., & Lehmann, J. M. (1995). A prostaglandin J2 metabolite binds peroxisome proliferator-activated receptor γ and promotes adipocyte differentiation. Cell, 83(5), 813-819.

    Article  CAS  PubMed  Google Scholar 

  • Kumar, R., & Chandra, M.D. (1996). Influence of palm oil on immune responses and susceptibility to infection in a mouse model. Nutrition Research, 16, 61-68.

    Article  Google Scholar 

  • Lamp, O., Reyer, H., Otten, W., Nürnberg, G., Derno, M., Wimmers, K., Metges, C.C. & Kuhla, B. (2018). Intravenous lipid infusion affects dry matter intake, methane yield, and rumen bacteria structure in late-lactating Holstein cows. Journal of Dairy Science 101, 6032-6046.

    Article  CAS  PubMed  Google Scholar 

  • Lee, J.Y., Kyung, H.S., Rhee, S.H. & Hwang, D. (2001). Saturated fatty acids, but not unsaturated fatty acids, induce the expression of cyclooxygenase-2 mediated through Toll-like receptor. Journal of Biological Chemistry, 276, 16683-16689.

    Article  CAS  PubMed  Google Scholar 

  • Lerma-Reyes, I., Mendoza-Martínez, G. D., Rojo-Rubio, R., Mejia, M., García-Lopez, J. C., & Lee-Rangel, H. A. (2018). Influence of supplemental canola or soybean oil on milk yield, fatty acid profile and postpartum weight changes in grazing dairy goats. Asian-Australasian Journal of Animal Sciences, 31(2), 225.

    Article  CAS  PubMed  Google Scholar 

  • Livak, K.J. & Schmittgen, T.D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2- ΔΔCT method. Methods 25, 402-408.

    Article  CAS  PubMed  Google Scholar 

  • Lundy Iii, F. P., Block, E., Bridges Jr, W. C., Bertrand, J. A., & Jenkins, T. C. (2004). Ruminal biohydrogenation in Holstein cows fed soybean fatty acids as amides or calcium salts. Journal of Dairy Science, 87(4), 1038-1046.

    Article  Google Scholar 

  • Ma, Y., Chen, X., Zahoor Khan, M., Xiao, J., Liu, S., Wang, J., Alugongo, G.M. & Cao, Z. (2022). Biodegradation and hydrolysis of rice straw with corn steep liquor and urea-alkali pretreatment. Frontier Nutrition 9, 989239.

  • Malik, A., & Kanneganti, T. D. (2018). Function and regulation of IL-1α in inflammatory diseases and cancer. Immunological Reviews, 281(1), 124-137.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • McKnight, I. M. (1966). A hematological study on the mountain whitefish, Prosopium williamsoni. Journal of the Fisheries Board of Canada, 23(1), 45-64.

    Article  CAS  Google Scholar 

  • Metcalf, L.C., Schmitz, A.A. & Pelka, J.R. (1966). Rapid preparation of methyl esters from lipid for gas chromatography analysis. Analytic. Chem 38, 514-515.

  • Monmai, C., Go, S.H., Shin, I.S., You, S., Kim, D.O., Kang, S., & Park, W.J. (2018). Anti-inflammatory effect of Asterias amurensis fatty acids through NF-κB and MAPK pathways against LPS-stimulated RAW264. 7 Cells, 28(10), 1635-1644.

  • Monteverde V., Congiu F., Vazzana I., Dara S., Di Pietro S. & Piccione G. (2017). Serum lipid profile modification related to polyunsaturated fatty acid supplementation in thoroughbred horses, Journal of Applied Animal Research, 45(1), 615-618.

    Article  CAS  Google Scholar 

  • Musazadeh, V., Jafarzadeh, J., Keramati, M., Zarezadeh, M., Ahmadi, M., Farrokhian, Z. & Ostadrahimi, A. (2021). Flaxseed oil supplementation augments antioxidant capacity and alleviates oxidative stress: a systematic review and meta-analysis of randomized controlled trials. Evidence-Based Complementary and Alternative Medicine, 12, 13-20.

    Google Scholar 

  • Panickar, K. S., & Jewell, D. E. (2015). The beneficial role of anti-inflammatory dietary ingredients in attenuating markers of chronic low-grade inflammation in aging. Hormone Molecular Biology and Clinical Investigation, 23(2), 59-70.

    Article  CAS  PubMed  Google Scholar 

  • Radzikowska, U., Rinaldi, A. O., Çelebi Sözener, Z., Karaguzel, D., Wojcik, M., Cypryk, K. & Sokolowska, M. (2019). The influence of dietary fatty acids on immune responses. Nutrients, 11(12), 2990.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Robinson, D. R., Urakaze, M., Huang, R., Taki, H., Sugiyama, E., Knoell, C. T. & Auron, P. E. (1996). Dietary marine lipids suppress continuous expression of interleukin-1β gene transcription1. Lipids, 31(1), S23-S31.

    Article  CAS  PubMed  Google Scholar 

  • Sadeghi, A.A., Safaei, A., & Aminafshar, M. (2013). The effects of dietary oil sources on performance, serum corticosterone level, antibody titers and IFN-γ gene expression in broiler chickens. Kafkas Universitesi Veteriner Fakultesi Dergisi, 20(6), 857-862.

  • Sears, B., & Saha, A. K. (2021). Dietary control of inflammation and resolution. Frontiers in Nutrition, 8. 709435.

    Article  PubMed  PubMed Central  Google Scholar 

  • Sembratowicz, I., Zięba, G., Cholewinska, E., & Czech, A. (2020). Effect of dietary flaxseed oil supplementation on the redox status, haematological and biochemical parameters of horses’ blood. Animals, 10(12), 2244.

  • Scarpa, G., Tarricone, S., & Ragni, M. (2021). Carcass composition, meat quality and sensory quality of Gentile di Puglia light lambs: Effects of dietary supplementation with oregano and flaxseed. Animals, 11(3), 607.

    Article  PubMed  PubMed Central  Google Scholar 

  • Shi, X.C., Jin, A., & Sun, J. (2017). α-lipoic acid ameliorates n−3 highly-unsaturated fatty acids induced lipid peroxidation via regulating antioxidant defenses in grass carp (Ctenopharyngodon idellus). Fish & Shellfish Immunology, 67, 359–367.

    Article  CAS  Google Scholar 

  • Steel, R.G.D., & Torrie, J.H. (1980). Principles and procedures of statistics. A biometrical approach, 2nd edn McGraw-Hill Book Company, New York. USA.

    Google Scholar 

  • Takano, H., Nagai, T., Asakawa, M., Toyozaki, T., Oka, T., Komuro, I. & Masuda, Y. (2000). Peroxisome proliferator–activated receptor activators inhibit lipopolysaccharide-induced tumor necrosis factor-α expression in neonatal rat cardiac myocytes. Circulation Research, 87(7), 596-602.

    Article  CAS  PubMed  Google Scholar 

  • van Soest, P.J., Robertson, J.B., & Lewis, B.A. (1991). Methods of dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74, 3583-3597.

  • Vinuesa, A., Pomilio, C., Gregosa, A., Bentivegna, M., Presa, J., Bellotto, M. & Beauquis, J. (2021). Inflammation and insulin resistance as risk factors and potential therapeutic targets for Alzheimer’s disease. Frontiers in Neuroscience, 15, 653651.

    Article  PubMed  PubMed Central  Google Scholar 

  • Voller, A. (1976). Microplate enzyme immunoassay for the immunodiagnosis of virus infection. Manual of Clinical Immunology, 1976, 506-512.

    Google Scholar 

  • Williams, A. R., Andersen-Civil, A. I., Zhu, L., & Blanchard, A. (2020). Dietary phytonutrients and animal health: regulation of immune function during gastrointestinal infections. Journal of Animal Science, 98(4), skaa030.

    Article  PubMed  PubMed Central  Google Scholar 

  • Wang, P., Anderson, P. O., Chen, S., Paulsson, K. M., Sjögren, H. O., & Li, S. (2001). Inhibition of the transcription factors AP-1 and NF-κB in CD4 T cells by peroxisome proliferator-activated receptor γ ligands. International Immunopharmacology, 1(4), 803-812.

    Article  CAS  PubMed  Google Scholar 

  • Xi, S., Cohen, D., Barve, S., & Chen, L. H. (2001). Fish oil suppressed cytokines and nuclear factor-kappaB induced by murine AIDS virus infection. Nutrition Research, 21(6), 865-878.

    Article  CAS  Google Scholar 

  • Xu, X., Otsuki, M., Saito, H., Sumitani, S., Yamamoto, H., Asanuma, N. & Kasayama, S. (2001). PPARα and GR differentially down-regulate the expression of nuclear factor-κB-responsive genes in vascular endothelial cells. Endocrinology, 142(8), 3332-3339.

    Article  CAS  PubMed  Google Scholar 

  • Yaqoob, P., Pala, H. S., Cortina-Borja, M., Newsholme, E. A., & Calder, P. C. (2000). Encapsulated fish oil enriched in alpha-tocopherol alters plasma phospholipid and mononuclear cell fatty acid compositions but not mononuclear cell functions. European Journal of Clinical Investigation, 30(3), 260-274.

    Article  CAS  PubMed  Google Scholar 

  • Zhang, R., Zhu, W., & Mao, S. (2016). High-concentrate feeding upregulates the expression of inflammation-related genes in the ruminal epithelium of dairy cattle. Journal of Animal Science and Biotechnology, 7(1), 1-13.

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The authors thank the Jahan Company for providing oils and Dr. Parvin Shawrang for her cooperation and generous assistance in carrying out this research project.

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Authors and Affiliations

  1. Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Morteza Rahimi-Tari, Ali Asghar Sadeghi, Mehdi Aminafshar & Mohammad Chamani

  2. Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, AEOI, Karaj, Iran

    Farahnaz Motamedi-Sedeh

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  1. Morteza Rahimi-Tari
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AAS and FMS designed research and revised the manuscript. MR conducted the experiments and wrote the initial format of manuscript. MC contributed in lab analysis and reading of manuscript. MAA analyzed the data. All authors reviewed and approved the manuscript.

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Correspondence to Ali Asghar Sadeghi.

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Rahimi-Tari, M., Sadeghi, A.A., Motamedi-Sedeh, F. et al. Hematological parameters, antioxidant status, and gene expression of γ-INF and IL-1β in vaccinated lambs fed different type of lipids. Trop Anim Health Prod 55, 168 (2023). https://doi.org/10.1007/s11250-023-03585-5

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