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Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows

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Abstract

Mastitis is the inflammation of the mammary gland due to microbial infiltration causing a reduced mammary function. This study aims at developing a vaccine using Malaysian local isolate of Staphylococcus aureus and evaluating serum amyloid A, Interleukin-10, IgM and IgG responses periodically. Four bacterin concentrations (106, 107, 108 and 109 cfu/ml of the local isolate of S. aureus) were adjuvanted with aluminium potassium sulphate. Thirty cows grouped into 4 treatment groups (G-) were vaccinated (2 ml) intramuscularly, with a fifth G-A as control. The mean concentration (MC) of serum amyloid A (SAA) was significantly different (sig-d) (p ˂ 0.05) in G-D at 0 h post vaccination (PV), 3 h PV, 24 h PV, weeks 1, 2, 3 and 4 PV (6-, 15-, 5-, 12-, 11-, 4- and 11-fold increased (FI) respectively). The MC of serum amyloid A was also sig-d in G-E at 0 h PV, weeks 1, 2 and 4 PV (3, 8, 5 and 8 FI respectively). The MC of IL-10 was sig-d in G-D and C at 3 h PV and week 2 PV (5 and 2 FI respectively). The IgM MC was sig-d in G-B and C at 3 h PV (5 and 6 FI respectively), at 24 h PV (5 and 9 FI respectively), at week 3 PV(2 and 2 FI respectively) and week 4 PV (3 and 4 FI respectively). The MC of IgG was sig-d in G-E at 0 h, 3 h and week 3 PV(5, 6 and 2 FI respectively) and in G-D at weeks 1–4 (3, 3, 3 and 5 FI respectively). In conclusion, elevated levels of SAA, IgG and IL-10 in G-D(108) informed our choice of best dosage which can be used to evoke immunity in cows.

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Acknowledgements

The authors wish to appreciate the management and staff of the Department of Clinical studies, Faculty of Veterinary Medicine, University Putra Malaysia (UPM) for their cooperation throughout the duration of the experimental trial.

Funding statement

The publication of this article was funded by Universiti Putra Malaysia and the grant number to this research is 9490500.

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Authors and Affiliations

  1. Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Idris Umar Hambali, Faez Firdaus Jesse Bin Abdullah, K. R. Bhutto, A. H. Wahid, M. N. Odhah & M. N. Jefri

  2. Department of Veterinary Public health and Preventive Medicine, University of Maiduguri, Maiduguri, 600233, Nigeria

    Idris Umar Hambali

  3. Research Centre for Ruminant Disease, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Faez Firdaus Jesse Bin Abdullah

  4. Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Faez Firdaus Jesse Bin Abdullah, M. Arsalan & N. A. Muhammad

  5. Directorate of Veterinary Research and Diagnosis, Livestock and Fisheries Department, Sindh, 70050, Pakistan

    K. R. Bhutto

  6. Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    M. L. Mohd Azmi & Z. Zakaria

  7. Department of Veterinary Medicine, Faculty of Agriculture and Veterinary Medicine, Thamar University, 39, Dhamar, Yemen

    M. N. Odhah

  8. Directorate of Animal Health, Livestock and Dairy Development Department Baluchistan, Lahore, 87300, Pakistan

    M. Arsalan

  9. Department of Clinical Medicine and Surgery, University of Veterinary and Animal Science Lahore, Lahore, 54500, Pakistan

    N. A. Muhammad

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  1. Idris Umar Hambali
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  2. Faez Firdaus Jesse Bin Abdullah
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  3. K. R. Bhutto
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  8. M. Arsalan
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Correspondence to Idris Umar Hambali or Faez Firdaus Jesse Bin Abdullah.

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The ethical approval for this vaccine trial was issued by the Institutional Animal Care and use committee (IACUC) of the Universiti Putra Malaysia referenced as UPM/IACUC/AUP- R072/2016.

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Hambali, I.U., Abdullah, F.F.J.B., Bhutto, K.R. et al. Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators, mediators and immunoglobulins in cows. Trop Anim Health Prod 51, 781–789 (2019). https://doi.org/10.1007/s11250-018-1755-8

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