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The diversity of BVDV subgenotypes in a vaccinated dairy cattle herd in Brazil

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Abstract

Bovine viral diarrhoea virus (BVDV) is an important pathogen of cattle that occurs worldwide with substantial economic impact on beef and dairy industries. The aim of this study was to describe the diversity of BVDV subgenotypes in persistently infected (PI) animals identified in a highly productive, regularly vaccinated, dairy cattle herd presenting with reproductive failure. Serum samples were collected from all animals within the herd (n = 692) and used to detect the presence of BVDV RNA. Using reverse transcription polymerase chain reaction assay, 29 cows were identified as transiently infected, three animals (two cows and one calf) as persistently infected, and one calf as putative BVDV PI animal. The sequences of 5'UTR and/or Npro gene of BVDV used in phylogenetic analyses revealed that the three PI animals were infected by three different BVDV subgenotypes (BVDV-1a, BVDV-1b, and BVDV-1d). These results demonstrated that in an open dairy cattle herd, regular vaccination against BVDV by itself is not able to prevent viral circulation in the herd. Furthermore, depending on the frequency of the acquisition of heifers and/or cows for replacement, several BVDV subgenotypes may co-exist simultaneously in the same herd.

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Acknowledgments

The authors thank the Brazilian Institutes CNPq, CAPES, FINEP, and Araucaria Foundation for financial support. A. F. Alfieri, S. A. Headley, and A. A. Alfieri are recipients of CNPq fellowships.

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Authors and Affiliations

  1. Laboratory of Animal Virology, Department of Veterinary Preventive Medicine, Universidade Estadual de Londrina, Paraná, Brazil

    Rodrigo A. A. Otonel, Alice F. Alfieri & Amauri A. Alfieri

  2. Laboratory of Molecular Biology, Instituto Federal Catarinense, Araquari, Santa Catarina, Brazil

    Stelamaris Dezen

  3. Laboratory of Veterinary Microbiology, Veterinary Teaching Hospital, Universidade de Cuiaba, Mato Grosso, Brazil

    Michele Lunardi

  4. Laboratory of Veterinary Pathology, School of Veterinary Medicine, Universidade Norte do Paraná, Arapongas, Paraná, Brazil

    Selwyn A. Headley

  5. Rodovia Celso Garcia Cid, Campus Universitário, PO Box 10.011, CEP 86057-970, Londrina, Paraná, Brazil

    Amauri A. Alfieri

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  1. Rodrigo A. A. Otonel
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  2. Alice F. Alfieri
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  4. Michele Lunardi
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  6. Amauri A. Alfieri
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Correspondence to Amauri A. Alfieri.

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Otonel, R.A.A., Alfieri, A.F., Dezen, S. et al. The diversity of BVDV subgenotypes in a vaccinated dairy cattle herd in Brazil. Trop Anim Health Prod 46, 87–92 (2014). https://doi.org/10.1007/s11250-013-0451-y

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