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Isolation and molecular characterization of infectious bronchitis virus from recent outbreaks in broiler flocks reveals emergence of novel strain in India

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Abstract

In this study, two isolates of infectious bronchitis virus (IBV) from field outbreaks in 2008 (India/LKW/56/IVRI/08) and 2010 (India/NMK/72/IVRI/10) from broiler chickens in India were isolated and characterized. Reverse transcription polymerase chain reaction–restriction fragment length polymorphism of the entire S1 gene revealed that these isolates belong to two different genotypes, India/LKW/56/IVRI/08 as Mass strain whereas India/NMK/72/IVRI/10 as of different genotype. Nucleotide sequencing analysis showed that India/LKW/56/IVRI/08 shared 99 % homology with THA280252 (Thailand) and India/NMK/72/IVRI/10 shared greater than 99 % homology with 4/91 pathogenic strain (UK), JP/Wakayama/2/2004 (Japan) and TA03 (China), while the two Indian IBV isolates shared 73 % identity between them. Phylogenetic data allowed classification of two Indian isolates, India/LKW/56/IVRI/08 as having unique lineage within Mass genotype and India/NMK/72/IVRI/10 as of 4/91 genotype. Our study confirmed the presence of 4/91 (793/B) IBV nephropathogenic strain for the first time in India by virus isolation and sequencing.

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Acknowledgments

The authors are thankful to the Indian Council of Agricultural Research for providing the necessary grants and facilities to carry out this research work.

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Authors and Affiliations

  1. Avian Diseases Section, Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, Uttar Pradesh, 243 122, India

    Vikshe Sumi, Shambhu Dayal Singh, Kuldeep Dhama, Vasudevan Gowthaman & Rajamani Barathidasan

  2. Department of Veterinary Microbiology, Veterinary College and Research Institute, Namakkal, Tamil Nadu, 637003, India

    Kuppannan Sukumar

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  1. Vikshe Sumi
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  2. Shambhu Dayal Singh
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  3. Kuldeep Dhama
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  4. Vasudevan Gowthaman
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  5. Rajamani Barathidasan
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  6. Kuppannan Sukumar
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Correspondence to Kuldeep Dhama.

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Sumi, V., Singh, S.D., Dhama, K. et al. Isolation and molecular characterization of infectious bronchitis virus from recent outbreaks in broiler flocks reveals emergence of novel strain in India. Trop Anim Health Prod 44, 1791–1795 (2012). https://doi.org/10.1007/s11250-012-0140-2

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