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Serological and molecular detection of Mycobacterium avium subsp. paratuberculosis in cattle of dairy herds in Colombia

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Abstract

The objective of this study is the detection of Mycobacterium avium subsp. paratuberculosis (MAP) by serum enzyme-linked immunosorbent assay (ELISA), fecal polymerase chain reaction (PCR), and fecal culture in Colombian dairy herds. Serum and fecal samples from asymptomatic cows (n = 307) of 14 dairy herds were tested for MAP by an unabsorbed ELISA test (ELISA-A). Serum and fecal samples from positive ELISA-A animals (n = 31) were further tested by an absorbed ELISA test (ELISA-B) and PCR. Fecal samples from animals of herds positive by ELISA-A and PCR (n = 105) were inoculated onto three different culture media. ELISA-A produced positive results in 10% of the serum samples and 71% of the herds. ELISA-B and PCR results were positive in two and six serum and fecal samples from positive ELISA-A animals, respectively. Fecal samples were negative for MAP on all culture media. The results of this study confirmed the presence of MAP in local dairy herds and the difficulties of MAP detection in asymptomatic animals by ELISA, PCR, and fecal culture.

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Acknowledgments

This study was partially funded by the ALECOL program (Universidad de Antioquia, German Academic Exchange Service-DAAD, Colciencias–Ministerio de Educación Nacional–Icetex). Authors thank M. Fischer, C. Walter, K. Simon, and K. Failing of the Justus Liebig University Giessen for scientific, technical, and statistical support. J. Reyes, LG. Palacio, F. Arango, and M. Palacio for scientific and logistic support, and Departamento de Formación Académica de Haciendas of the Universidad de Antioquia, as well as participating farmers for their cooperation.

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Authors and Affiliations

  1. Escuela de Medicina Veterinaria (Grupo Centauro), Facultad de Ciencias Agrarias, Universidad de Antioquia, Carrera 75 #65-87, Medellín, Colombia

    Jorge Arturo Fernández-Silva

  2. Institut für Tierärztliche Nahrungsmittelkunde, Fachbereich Veterinärmedizin, Justus–Liebig–Universität Giessen, Frankfurter Straße 92, 35392, Giessen, Germany

    Jorge Arturo Fernández-Silva, Amir Abdulmawjood, Ömer Akineden & Michael Bülte

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  1. Jorge Arturo Fernández-Silva
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  2. Amir Abdulmawjood
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  3. Ömer Akineden
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  4. Michael Bülte
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Correspondence to Jorge Arturo Fernández-Silva.

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Fernández-Silva, J.A., Abdulmawjood, A., Akineden, Ö. et al. Serological and molecular detection of Mycobacterium avium subsp. paratuberculosis in cattle of dairy herds in Colombia. Trop Anim Health Prod 43, 1501–1507 (2011). https://doi.org/10.1007/s11250-011-9833-1

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