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A preliminary study on using autologous and heterologous boar sperm supernatant from freezing processes as post-thawing solution: its effect on sperm motility

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Abstract

The aim of this study was to evaluate the effect of post-thawing dilution with autologous and heterologous sperm supernatant on motility of frozen-thawed boar spermatozoa. During the cryopreservation, sperm supernatant (a combination of seminal plasma and semen extender, 50% v/v) or seminal plasma from nine boars (Duroc, Large White, and Landrace; three in each) was collected by centrifugation and stored frozen until use as post-thawing solution. Sperm pellets were further processed and cryopreserved using control-rate freezer and was thawed at 50°C for 12 s. After thawing, frozen thawed semen samples were diluted with seminal plasma (group A), supernatant from Landrace (group B), supernatant from Large White (group C), supernatant from Duroc (group D), and Modena™ semen extender (group E). Post-thawing motility was evaluated using a phase-contrast microscope after thawing at 1, 10, 20, and 30 min. The present results show that at 1 min, a significantly higher percentage (P ≤ 0.001) of progressive motility was found in groups B (53.3%) and C (53.9%) than the other groups. At 10 min, the highest (P ≤ 0.001) progressive motility was found in groups B (65%) and C (61%). At 20 and 30 min, a significantly higher percentage (P ≤ 0.001) of progressive motility was found in groups B (58.9%), C (53.5%), and D (45.6%) than groups A (3.9%) and E (20.6%). It can be stated that supernatant from the freezing processes (consisting of seminal plasma and Modena™, 50% v/v) had a beneficial effect on post-thawing progressive motility of frozen boar semen.

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Acknowledgments

Research grants for this study were provided by the Thailand Research Fund (MRG 4980040 and CHETRF Senior Research Fund, RTA 5080010) and National Research Council of Thailand (NRCT).

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Authors and Affiliations

  1. Semen Laboratory, Faculty of Veterinary Science, Mahidol University, Nakorn-pathom, Thailand, 73170

    Kampon Kaeoket & Panida Chanapiwat

  2. Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand, 10330

    Panida Chanapiwat, Padet Tummaruk, Mongkol Techakumphu & Annop Kunavongkrit

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  1. Kampon Kaeoket
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  2. Panida Chanapiwat
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Correspondence to Kampon Kaeoket.

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Kaeoket, K., Chanapiwat, P., Tummaruk, P. et al. A preliminary study on using autologous and heterologous boar sperm supernatant from freezing processes as post-thawing solution: its effect on sperm motility. Trop Anim Health Prod 43, 1049–1055 (2011). https://doi.org/10.1007/s11250-011-9804-6

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