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L-phenylalanine applications and culture duration affect root growth and production of tropane alkaloids and phenolics in adventitious root cultures of Hyoscyamus niger L.

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Abstract

This study was aimed to determine the effects of different concentrations of L-phenylalanine (0.25, 0.50 and 1.00 mM L-Phe) applied to adventitious root cultures for different culture durations on root growth and production of tropane alkaloids and phenolics in Hyoscyamus niger. For this purpose, adventitious roots obtained from petiole explants of in vitro seedlings were used as plant materials. After adventitious roots were cultured in liquid Murashige and Skoog (MS) medium, L-Phe was applied to the adventitious roots. Then the roots were harvested on the 1st, 3rd, and 7th day of the applications, fresh and dry root weights, root growth indexes were analysed. It was also examined the contents of tropane alkaloids (hyoscyamine and scopolamine) and phenolics (gallic acid, catechin, epicatechin, vanillin, cinnamic acid, rosmarinic acid, p-coumaric, o-coumaric acid, rutin, caffeic acid, ferulic acid, chlorogenic acid, and quercetin) by HPLC in both adventitious roots and culture medium. As a result, according to root growth parameters data, day*application interaction was not found statistically significant. The maximum scopolamine and hyoscyamine amounts were detected in the cultures with the application of 0.50 mM L-Phe for 3 days. In terms of phenolics, 0.50 mM and 1.00 mM L-Phe application with 1st and 3rd days were selected as the most appropriate L-Phe applications and culture duration. Consequently, it has been determined that in vitro secondary metabolite production may be increased in H. niger root cultures with the selection of appropriate L-Phe concentrations and culture durations.

Key Message

L-phenylalanine is an effective and inexpensive application for the production of secondary metabolites in adventitious root cultures of Hyoscyamus niger with the correct concentration and appropriate culture duration.

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Funding

This study was funded by the Scientific and Technological Research Council of Turkey (TUBITAK, TOVAG, 1002, Project No: 218O196).

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Authors and Affiliations

  1. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Suleyman Demirel University, 32260, Isparta, Turkey

    Tunhan Demirci

  2. Department of Agricultural Biotechnology, Faculty of Agriculture, Isparta University of Applied Sciences, 32270, Isparta, Turkey

    İlknur Albayrak & Nilgün Göktürk Baydar

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  1. Tunhan Demirci
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  2. İlknur Albayrak
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  3. Nilgün Göktürk Baydar
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Contributions

TD: Conceptualization, Methodology, Formal analysis, Investigation, Writing—original draft, Writing—review & editing, Supervision, Funding acquisition. IA: Formal analysis, Investigation. NGB: Writing—review & editing, Supervision, Resources, Funding acquisition, Project administration.

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Correspondence to Tunhan Demirci.

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Communicated by Konstantin V. Kiselev.

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Demirci, T., Albayrak, İ. & Göktürk Baydar, N. L-phenylalanine applications and culture duration affect root growth and production of tropane alkaloids and phenolics in adventitious root cultures of Hyoscyamus niger L.. Plant Cell Tiss Organ Cult 149, 295–311 (2022). https://doi.org/10.1007/s11240-021-02173-5

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