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Production of monoclonal antibody against FimA protein from Porphyromonas gingivalis in rice cell suspension culture

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Abstract

Major fimbriae (FimA) of Porphyromonas gingivalis play an important role in the adherence of the bacterium to oral surfaces, making them a target for the development of a vaccine against periodontal disease. In our previous study, monoclonal antibodies to FimA were expressed in tobacco plants using the agroinfiltration method. In this report, for easy purification, monoclonal antibodies to FimA were produced and accumulated in rice callus suspension culture. The accumulated antibodies were purified by protein G-affinity chromatography. The plant-produced monoclonal antibodies inhibited the binding of P. gingivalis to saliva-coated hydroxyapatite beads, as well as the invasion of oral epithelial KB cells due to the bacterium. The antibodies enhanced the intracellular killing of P. gingivalis by polymorphonuclear neutrophils. These results suggest that FimA-specific monoclonal antibodies produced in a rice suspension culture were easily purified and biologically active against P. gingivalis, and thus may be used for passive immunization to prevent P. gingivalis-induced periodontal disease.

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Acknowledgments

This study was supported by the Bio-industry Technology Development Program, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea.

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Authors and Affiliations

  1. Department of Molecular Biology, Chonbuk National University, Chonju, 561-756, Republic of Korea

    Bang-Geul Kim, Sae-Hae Kim, Nan-Sun Kim, Yong-Suk Jang, Moon-Sik Yang & Tae-Geum Kim

  2. Department of Techno-pedagogy, Hue University’s College of Education, 34 Le Loi St., Hue, Vietnam

    Nguyen-Xuan Huy

  3. Institute of Oral Biology, Kyung Hee University, Seoul, 130-701, Republic of Korea

    Young-Suk Choi & Jin-Yong Lee

  4. Institute of Molecular Biology and Genetics, Chonbuk National University, Chonju, 561-756, Republic of Korea

    Yong-Suk Jang & Moon-Sik Yang

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  1. Bang-Geul Kim
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Correspondence to Tae-Geum Kim.

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Kim, BG., Kim, SH., Kim, NS. et al. Production of monoclonal antibody against FimA protein from Porphyromonas gingivalis in rice cell suspension culture. Plant Cell Tiss Organ Cult 118, 293–304 (2014). https://doi.org/10.1007/s11240-014-0481-9

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  • DOI: https://doi.org/10.1007/s11240-014-0481-9

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