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Efficient regeneration and antioxidative enzyme activities in Brassica rapa var. turnip

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Abstract

The regeneration potential and antioxidative enzyme activities of economically important Brassica rapa var. turnip were evaluated. Calli were induced from leaf explants of seed-derived plantlets on Murashige and Skoog (MS) medium incorporated with different concentrations of various plant growth regulators (PGRs). The highest leaf explant response (83%) was recorded for 2.0 mg l−1 benzyladenine (BA) and 1.0 mg l−1 α-naphthaleneacetic acid (NAA). Subsequent subculturing of callus after 3 weeks of culture, on medium with similar compositions of PGRs, induced shoot organogenesis. The highest shoot induction response (83%) was recorded for 5.0 mg l−1 BA after 5 weeks of transfer. However, 7.8 shoots/explant were recorded for 2.0 mg l−1 BA. The transferring of shoots to elongation medium resulted in 5.1-cm-long shoots on 10 mg l−1 of gibberellic acid (GA3). Rooted plantlets were obtained on MS medium containing different concentrations of indole butyric acid (IBA). The determination of activities of antioxidative enzymes (superoxide dismutase [SOD], ascorbate peroxidase [APX], catalase [CAT], glutathione peroxidase [GPX], and peroxidase [POD]) revealed involvement of these enzymes in callus formation and differentiation. All of the activities were interlinked with each other and played significant roles in the scavenging of toxic free radicals. This study will help in the advancement of a regeneration protocol for B. rapa var. turnip and the understanding of the functions of antioxidative enzymes in plant differentiation.

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Abbreviations

BA:

6-Benzyladenine

IBA:

Indole butyric acid

GA3 :

Gibberellic acid

MS:

Murashige and Skoog medium

MS0:

MS medium without plant growth regulators

NAA:

α-Naphthaleneacetic acid

PGRs:

Plant growth regulators

SOD:

Superoxide dismutase

CAT:

Catalase

APX:

Ascorbate peroxidase

GPX:

Glutathione peroxidase

POD:

Peroxidase

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Acknowledgments

Dr. Bilal Haider Abbasi acknowledges the support of the University Research Fund (URF 2010–2011) of Quaid-i-Azam University, Islamabad, Pakistan.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, 45320, Pakistan

    Bilal Haider Abbasi, Murad Khan & Mir Ajab Khan

  2. College of Life Sciences, Northwest University, North Taibai Road, 710069, Xi’an, People’s Republic of China

    Bin Guo

  3. Department of Chemistry, GC University, Lahore, 54000, Pakistan

    Saleem Ahmed Bokhari

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  1. Bilal Haider Abbasi
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  2. Murad Khan
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  3. Bin Guo
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  4. Saleem Ahmed Bokhari
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  5. Mir Ajab Khan
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Correspondence to Bilal Haider Abbasi.

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Abbasi, B.H., Khan, M., Guo, B. et al. Efficient regeneration and antioxidative enzyme activities in Brassica rapa var. turnip . Plant Cell Tiss Organ Cult 105, 337–344 (2011). https://doi.org/10.1007/s11240-010-9872-8

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  • DOI: https://doi.org/10.1007/s11240-010-9872-8

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