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In vitro propagation of Huernia hystrix: an endangered medicinal and ornamental succulent

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Abstract

An efficient and rapid method for in vitro clonal propagation of Huernia hystrix was developed, resulting in shoot regeneration within 3 weeks of culture. This endangered medicinal and ornamental succulent is in high demand. Multiple shoots were regenerated from stem explants (10 mm length) cultured on Murashige and Skoog (MS) medium containing 3% sucrose and supplemented with a range of NAA (0.00–8.06 μM) and BA (4.44–22.19 μM) concentrations. A 100% shoot response with a multiplication rate of four shoots per explant was obtained on MS medium containing 5.37 μM NAA and 22.19 μM BA. Callus produced at the base of the explant on the same medium showed root organogenic potential. The in vitro regenerated shoots produced roots when transferred to half strength MS medium with or without auxin. The micropropagated plants were easily acclimatized within 2 months under greenhouse conditions when potted in a soil and sand mixture (1:1; v/v) treated with a fungicide (Benlate, 0.01%). More than 95% survival with no observable morphological variations was obtained. The developed protocol provides a simple, cost-effective means for the conservation of endangered H. hystrix by clonal propagation within a short time.

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Abbreviations

BA:

6-Benzylaminopurine

IBA:

Indole butyric acid

MS:

Murashige and Skoog (1962) medium

NAA:

α-Naphthalene acetic acid

PGR:

Plant growth regulator

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Acknowledgements

Garry Stafford of the Research Centre for Plant Growth and Development, University of KwaZulu-Natal Pietermaritzburg is thanked for assistance in plant collection. The National Research Foundation and the University of KwaZulu-Natal are gratefully acknowledged for financial support.

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Authors and Affiliations

  1. Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, South Africa

    S. O. Amoo, J. F. Finnie & J. Van Staden

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  1. S. O. Amoo
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  2. J. F. Finnie
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  3. J. Van Staden
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Correspondence to J. Van Staden.

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Amoo, S.O., Finnie, J.F. & Van Staden, J. In vitro propagation of Huernia hystrix: an endangered medicinal and ornamental succulent. Plant Cell Tiss Organ Cult 96, 273–278 (2009). https://doi.org/10.1007/s11240-008-9484-8

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  • DOI: https://doi.org/10.1007/s11240-008-9484-8

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