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Identification of differentially expressed sequences in pre-embryogenic tissue of oilseed rape by suppression subtractive hybridisation (SSH)

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Abstract

A subtracted cDNA library comprising of 576 clones were constructed to isolate differentially expressed sequences in the pre-embryogenic tissue (PEC) of winter oilseed repe. After differential screening, only 16 clones were identified as potential positives. Eventually, only three clones: BNPE DG3, BNPE AE4 and BNPE EG1 were confirmed by Northern analysis as upregulated in␣PEC of the oilseed rape embryogenic culture. This is the first study to report Ae4, Dg3 and Eg1 sequences as preferentially expressed in the oilseed␣rape PEC and associated with the induction of somatic embryogenesis in B.napus secondary embryogenesis. Ae4 encodes a putative proline-rich protein, Dg3 encodes a lipid transfer-like protein and Eg1 encodes a napin, member of the BNMNAP subfamily.

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Abbreviations

SSH:

Suppression subtractive hybridisation

NEC:

Non-embryogenic tissue

PEC:

Pre-embryogenic tissue

BNPE:

Brassica napus pre-embryogenic

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Authors and Affiliations

  1. Department of Cell and Biomolecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia

    Parameswari Namasivayam

  2. Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge, CB2 3EA, UK

    David Hanke

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  1. Parameswari Namasivayam
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  2. David Hanke
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Correspondence to Parameswari Namasivayam.

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Namasivayam, P., Hanke, D. Identification of differentially expressed sequences in pre-embryogenic tissue of oilseed rape by suppression subtractive hybridisation (SSH). Plant Cell Tiss Organ Cult 86, 417–421 (2006). https://doi.org/10.1007/s11240-006-9141-z

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  • DOI: https://doi.org/10.1007/s11240-006-9141-z

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