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Constitutive expression of two pathogenesis-related genes in tomato plants enhanced resistance to oomycete pathogen Phytophthora capsici

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Abstract

The potential role of two pathogen-induced pepper genes, encoding basic pathogenesis-related protein 1 (CABPR1) and ascorbate peroxidase-like 1 (CAPOA1), in tolerance against phytopathogens was examined in transgenic tomato (Lycopersicum esculentum cv. House Momotaro) plants. Polymerase chain reaction and reverse transcription-polymerase chain reaction analyses using gene-specific primers revealed that the pepper CABPR1 and CAPOA1 genes were integrated into the tomato genome. The constitutive expression of CABPR1 and CAPOA1 in the tomato did not exhibit any morphological abnormalities. However, these transgenic tomato plants showed enhanced tolerance to the oomycete pathogen Phytophthora capsici, and very weak resistance to the bacterial pathogen Pseudomonas syringae pv. tomato. These results suggest that overexpression of CABPR1 and CAPOA1 in tomato plants altered their resistance responses to pathogenic attack.

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Acknowledgement

This study was supported by the special grant from the Agricultural R&D Promotion Center funded by the Ministry of Agriculture and Forestry.

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Authors and Affiliations

  1. School of Life Sciences and Biotechnology, Korea University, 136-701, Seoul, Korea

    Sujon Sarowar, Young Jin Kim & Jeong Sheop Shin

  2. College of Life and Environmental Sciences, Korea University, 136-701, Seoul, Korea

    Eui Nam Kim & Ki Deok Kim

  3. Department of Plant Science and Technology, Sangmyung University, 330-720, Cheonan, Korea

    Jun Young Choi & Nam In Hyung

Authors
  1. Sujon Sarowar
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  2. Young Jin Kim
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  3. Eui Nam Kim
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  4. Ki Deok Kim
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  5. Jun Young Choi
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  6. Nam In Hyung
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  7. Jeong Sheop Shin
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Correspondence to Jeong Sheop Shin.

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Sarowar, S., Kim, Y.J., Kim, E.N. et al. Constitutive expression of two pathogenesis-related genes in tomato plants enhanced resistance to oomycete pathogen Phytophthora capsici . Plant Cell Tiss Organ Cult 86, 7–14 (2006). https://doi.org/10.1007/s11240-006-9090-6

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  • DOI: https://doi.org/10.1007/s11240-006-9090-6

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