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Protoplast Fusion in Banana (Musa spp.): Comparison of Chemical (PEG: Polyethylene Glycol) and Electrical Procedure

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Abstract

Optimization of protoplast fusion parameters is a prerequisite for the establishment of somatic fusion technology for banana breeding. In the present investigations, we compared the most frequently used fusion methods: the electrofusion technique and chemical procedure (polyethylene glycol). With regard to frequency of binary fusion, protoplast fusion with the fusogen polyethylene glycol was best. Conversely, electric fusion was found to be better with respect to mitotic activities, somatic embryogenesis and plantlet regeneration rate.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

BA:

benzylaminopurine

CIRAD:

Center for International Cooperation in Agricultural Research for Development

IAA:

indole-3-acetic acid

MES:

2-(N-morpholino) ethanesulfonic acid

NAA:

α-naphthaleneacetic acid

PCV:

packed cell volume

PEG:

polyethylene glycol

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Authors and Affiliations

  1. Department of Plant Agriculture, University of Guelph, Bovey Building, N1G 2W1, Ontario, Guelph, Canada

    Akym Assani

  2. Faculté des Sciences Biologiques, Université des Sciences et de Technologie, B.P: 32, El Alia, 16111, Alger, Algérie

    Djamila Chabane

  3. Ecologie, Systématique et Evolution, Université de Paris Sud XI, UMR 8079, Bâtiment 362, F-91405, Orsay, Cedex, France

    Robert Haïcour

  4. CIRAD-FLHOR, TA 50/PS4, Boulevard de la Lironde, F-34398, Montpellier Cedex 5, France

    Frédéric Bakry

  5. Lehrstuhl für Pflanzenbau und Pflanzenzüchtung, Technische Universität München, D-85350, Freising-Weihenstephan, Germany

    Gerhard Wenzel

  6. Institut für Resistenzgenetik, Bundesanstalt für Züchtungsforschung an Kulturpflanzen, Graf-Seinsheim-Str. 23, D-85461, Grünbach, Germany

    Bärbel Foroughi-Wehr

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  1. Akym Assani
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  2. Djamila Chabane
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  3. Robert Haïcour
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  4. Frédéric Bakry
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  5. Gerhard Wenzel
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  6. Bärbel Foroughi-Wehr
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Correspondence to Akym Assani.

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Assani, A., Chabane, D., Haïcour, R. et al. Protoplast Fusion in Banana (Musa spp.): Comparison of Chemical (PEG: Polyethylene Glycol) and Electrical Procedure. Plant Cell Tiss Organ Cult 83, 145–151 (2005). https://doi.org/10.1007/s11240-005-4633-9

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  • DOI: https://doi.org/10.1007/s11240-005-4633-9

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