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Platelet-derived microparticle count and surface molecule expression differ between subjects with and without type 2 diabetes, independently of obesity status

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Abstract

This study investigated the impact of either type 2 diabetes or obesity, separately or in combination, on the absolute amounts of microparticles (MP) and the pathways by which these are associated with either condition. The concentrations of circulating MP derived from platelets (PMP), leukocytes (LMP) and monocytes (MMP), together with their specific activation markers, were compared in 30 subjects who were characterised across 4 cohorts as obese or type 2 diabetes. The subjects with type 2 diabetes had elevated concentrations of total PMP (P = 0.003), and PMP that were fibrinogen-positive (P = 0.04), tissue factor-positive (P < 0.001), P-selectin-positive (P = 0.03). Type 2 diabetes did not alter either total or activated LMP or MMP. Obesity per se did not impact on any MP measurement. Elevated concentrations of plasma PMP occurred in subjects with type 2 diabetes, whether they were obese or non-obese. In contrast, obesity in the absence of type 2 diabetes had no effect. The increased concentrations of specific marker-positive PMP in the subjects with diabetes might reflect potential pathways by which PMP may contribute to the pathogenesis of atherosclerosis and type 2 diabetes.

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Acknowledgments

This study was partly funded by a Grant awarded by the Chief Scientists Office of the Scottish Government to the University of the Highlands and Islands, Rowett Institute of Nutrition & Health at the University of Aberdeen and Aberdeen Royal Infirmary to examine the impact of diets rich in oats for lifestyle control of type 2 diabetes. Additional support was contributed by the Rural & Environment Science & Analytical Services (RESAS) of the Scottish Government to Rowett Institute of Nutrition & Health (RINH). Costs of microparticle analyses were borne by Provexis Plc.

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Authors and Affiliations

  1. Rowett Institute of Nutrition and Health, University of Aberdeen, Aberdeen, UK

    Xuguang Zhang, Alexandra M. Johnstone, Alan A. Sneddon, Baukje De Roos & Gerald E. Lobley

  2. Department of Diabetes, Aberdeen Royal Infirmary, Aberdeen, UK

    Susan C. McGeoch, Donald W. M. Pearson & Prakash Abraham

  3. Biomathematics and Statistics Scotland, Rowett Institute of Nutrition & Health, Aberdeen, UK

    Grietje Holtrop

  4. Department of Diabetes and Cardiovascular Science, University of the Highlands and Islands, Inverness, UK

    Sandra M. MacRury & Ian L. Megson

  5. Provexis Plc, c/o University of Aberdeen, Rowett Institute of Nutrition & Health, Greenburn Road, Bucksburn, Aberdeen, AB219SB, UK

    Niamh O’Kennedy

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  1. Xuguang Zhang
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  2. Susan C. McGeoch
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  10. Baukje De Roos
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Correspondence to Niamh O’Kennedy.

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Zhang, X., McGeoch, S.C., Johnstone, A.M. et al. Platelet-derived microparticle count and surface molecule expression differ between subjects with and without type 2 diabetes, independently of obesity status. J Thromb Thrombolysis 37, 455–463 (2014). https://doi.org/10.1007/s11239-013-1000-2

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  • DOI: https://doi.org/10.1007/s11239-013-1000-2

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