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Human platelet alloantigens HPA-1, HPA-2, and HPA-3 polymorphisms associated with extent of severe coronary artery disease

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Abstract

The contribution of human platelet antigen (HPA)-1 (GPIIb/IIIa), HPA-2 (GPIb/IX), and HPA-3 (GPIIb/IIIa) polymorphisms to the risk of coronary artery disease (CAD) was investigated in 341 CAD patients and 316 matched control subjects. HPA genotyping was performed by PCR-SSP. Regression analysis was employed in assessing the contribution of these variants to CAD risk. The frequency of HPA-1b (P = .009) and HPA-3b (P = .004) alleles, and HPA-1a/1b (P = .045), HPA-1b/1b (P = .007), and HPA-3b/3b (P = .008) genotypes were higher in patients than control subjects. No significant association was demonstrated between the HPA variants and 1-, 2- and 3-vessel disease. HPA-1b/2a/3b (Pc = .021) and HPA-1b/2b/3a (Pc = .002) haplotypes were positively associated with CAD, thereby conferring a disease susceptibility nature to these haplotypes. Multivariate analysis confirmed the positive association of HPA-1b/2a/3b (aOR = 3.72; 95% CI = 1.49–9.28), and in addition identified HPA-1b/2a/3a (aOR = 2.49; 95% CI = 1.06–5.86) to be positively associated with CAD, after adjusting for a number of covariates. Our results demonstrate positive association of HPA variants and specific HPA-1/HPA-2/HPA-3 haplotypes with CAD in Tunisians.

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Authors and Affiliations

  1. Research unit of Hematological and Autoimmune Diseases, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia

    Nesrine Abboud, Lakdhar Ghazouani, Sonia Ben-Hadj-Khalifa & Touhami Mahjoub

  2. Cardiology Department, CHU Menzel Bourguiba, Monastir, Tunisia

    Fatma Anabi & Ali Khalfallah

  3. Cardiology Department, CHU Fattouma Bourguiba, Monastir, Tunisia

    Faouzi Added

  4. Pathology Department, Military Hospital, Tunis, Tunisia

    Brahim Nsiri

  5. Department of Medical Biochemistry, College of Medicine and Medical Sciences, Arabian Gulf University, PO Box 22979, Manama, Bahrain

    Wassim Y. Almawi

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  1. Nesrine Abboud
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  2. Lakdhar Ghazouani
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Correspondence to Wassim Y. Almawi.

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Abboud, N., Ghazouani, L., Ben-Hadj-Khalifa, S. et al. Human platelet alloantigens HPA-1, HPA-2, and HPA-3 polymorphisms associated with extent of severe coronary artery disease. J Thromb Thrombolysis 29, 409–415 (2010). https://doi.org/10.1007/s11239-009-0368-5

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