Abstract
The domestic pig louse Haematopinus suis (Linnaeus, 1758) (Phthiraptera: Anoplura) is a common ectoparasite of domestic pigs, which can act as a vector of various infectious disease agents. Despite its significance, the molecular genetics, biology and systematics of H. suis from China have not been studied in detail. In the present study, the entire mitochondrial (mt) genome of H. suis isolate from China was sequenced and compared with that of H. suis isolate from Australia. We identified 37 mt genes located on nine circular mt minichromosomes, 2.9 kb-4.2 kb in size, each containing 2-8 genes and one large non-coding region (NCR) (1,957 bp-2,226 bp). The number of minichromosomes, gene content, and gene order in H. suis isolates from China and Australia are identical. Total sequence identity across coding regions was 96.3% between H. suis isolates from China and Australia. For the 13 protein-coding genes, sequence differences ranged from 2.8%-6.5% consistent nucleotides with amino acids. Our result is H. suis isolates from China and Australia being the same H. suis species. The present study determined the entire mt genome of H. suis from China, providing additional genetic markers for studying the molecular genetics, biology and systematics of domestic pig louse.
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Data availability
The complete mitochondrial genome of Haematopinus suis from China have been deposited in the GenBank under the accession numbers ON585586-ON585594.
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This study was supported in part, by the National Natural Science Foundation of China (Grant No. 32172884).
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GHL and RL conceived and designed the study, and critically revised the manuscript. RL and YN performed the experiments. RL YTF and YPD analyzed the data. RL YTF and YPD drafted the manuscript. WW and PPM helped in study design, study implementation, and manuscript preparation. All authors read and approved the final manuscript.
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All procedures involving animals in the present study were approved and this study was approved by the Animal Ethics Committee of Hunan Agricultural University (No. 43321503).
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11230_2023_10106_MOESM1_ESM.tif
Supplementary file1 Fig. S1 PCR verification of the nine mt minichromosomes of the Haematopinus suis from China. Lane M: DL5000 DNA marker; Lane M0: Negative control, Lane M1–M9: trnD-trnY-cox2-trnS1-trnS2-trnP-cox3-trnA, trnE-cytb-trnV, trnH-nad5-trnF, trnK-nad4-atp8-atp6-trnN, trnL1-rrnL, nad2-trnI-cox1-trnL2, trnQ-nad1-trnT-trnG-nad3-trnW, trnR-nad4L-nad6-trnM, rrnS-trnC. (TIF 15490 KB)
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Li, R., Nie, Y., Fu, YT. et al. Characterization of the fragmented mitochondrial genome of domestic pig louse Haematopinus suis (Insecta: Haematopinidae) from China. Syst Parasitol 100, 571–578 (2023). https://doi.org/10.1007/s11230-023-10106-3
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DOI: https://doi.org/10.1007/s11230-023-10106-3