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Henneguya michiganensis n. sp. (Cnidaria: Myxosporea) from the gills of muskellunge Esox masquinongy Mitchill(Esociformes: Esocidae)

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Abstract

Henneguya Thélohan, 1892 is the second most species rich genus of myxozoans, with reports from freshwater and marine fish worldwide. In the Great Lakes region of North America, muskellunge Esox masquinongy is an important game fish species that serves as an apex predator in the ecosystems of many inland lakes. The myxozoan fauna of esocid fish, especially muskellunge, remains largely understudied. During fish health assessments, muskellunge were examined for parasitic infections and myxozoan pseudocysts were observed on gill clip wet mounts. When ruptured under pressure, the intralamellar pseudocysts released thousands of myxospores consistent with those of the genus Henneguya. The myxospores were 67.3–96.6 (79.1 ± 5.9) µm in total length. The spore body was 18.6–22.6 (20.9 ± 1.0) µm × 5.4–6.9 (6.3 ± 0.4) µm in valvular view and 3.5–4.0 (3.8 ± 0.3) µm wide in sutural view. The two pyriform polar capsules positioned at the anterior of the spore body were 6.4–7.7 (7.0 ± 0.4) µm × 1.8–2.1 (2.0 ± 0.1) µm and each contained a tightly coiled polar filament with 9–10 turns. Two tapering caudal processes extended from the posterior of the spore body and were 47.3–75.6 (58.3 ± 5.8) µm in length. Histologically, large intralamellar polysporic plasmodia were surrounded by plump pillar cells and a distinct layer of plasma. Mild inflammation was present peripherally, with small numbers of necrotic germinative cells and intraplasmodial phagocytes internally. Ribosomal 18S rRNA gene sequence data were obtained from three gill pseudocysts. The three ~2000-bp sequences were identical, but shared no significant similarity with any publicly available sequence data. Phylogenetic analyses demonstrated sequence data from this Henneguya fell within a well-supported clade of Henneguya spp. reported from northern pike Esox lucius in Europe. Based on the distinct morphological, histological and molecular data, this species is designated as Henneguya michiganensis n. sp. from muskellunge in Michigan, USA.

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Acknowledgements

The authors thank Michigan Department of Natural Resources personnel for fish collections, particularly Mr. Todd Summers and Mr. Matt Hughes. The authors also thank past and present members of the Michigan State University – Aquatic Animal Health Laboratory for technical assistance.

Funding

This research was supported by the Michigan Department of Natural Resources and the Mississippi State University College of Veterinary Medicine.

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Authors and Affiliations

  1. Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi, MS, 39762, USA

    Thomas G. Rosser

  2. Department of Fisheries and Wildlife, College of Agriculture and Natural Resources, Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI, 48824, USA

    Thomas P. Loch & Mohamed Faisal

  3. Veterinary Diagnostic Laboratory, College of Veterinary Medicine, University of Illinois, Urbana, IL, 61802, USA

    Wes A. Baumgartner

  4. Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Stoneville, MS, 38776, USA

    Matt J. Griffin

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Correspondence to Thomas P. Loch.

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All applicable institution, national and international guidelines for the care and use of animals were followed. All fish handling and processing was performed in accordance with Michigan State University Institutional Animal Care and Use Committee (IACUC) standards (AUF 07/12-124-99).

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This article was registered in the Official Register of Zoological Nomenclature (ZooBank) as urn:lsid:zoobank.org:pub:6E88B072-457F-46C6-8E6A-B23E2CBE8D07. This article was published as an Online First article on the online publication date shown on this page. The article should be cited by using the doi number. This is the version of record.

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Rosser, T.G., Loch, T.P., Faisal, M. et al. Henneguya michiganensis n. sp. (Cnidaria: Myxosporea) from the gills of muskellunge Esox masquinongy Mitchill(Esociformes: Esocidae). Syst Parasitol 98, 119–130 (2021). https://doi.org/10.1007/s11230-021-09965-5

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