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Reclamation of beneficial bioactivities of herbal antioxidant condensed tannin extracted from Euonymus laxiflorus

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Abstract

Natural condensed tannins (CT) have received great interest due to their vast beneficial bioactivities. Recently, Euonymus laxiflorus, an edible medicinal herb collected in the Central Highlands of Vietnam, was newly recognized as a natural source rich in antioxidant CT. The aim of this study is to scale up extraction and investigate various novel bioactivities of the isolated CT. The screening of the parts used rich in CT, such as heartwood, trunk bark, branch, and the leaves of EL, indicated that the EL trunk bark (ELTB) has the highest CT content. E. laxiflorus CT (ELCT) was extracted with high-level scale from ELTB via several techniques and then was qualified by NMR spectra and HPLC finger printing. A condensed tannin ELBT-3.1.d was isolated and showed its high molecular weight of ≥ 10 kDa and various in vitro bioactivities. ELBT-3.1.d demonstrated high antioxidant capacity (IC50 = 6.11 µg/mL), and potent enzymes inhibition targets in anti-diabetes, especially showing novel potential inhibition against human salivary α-amylase with IC50 value of 3.01 µg/mL. ELBT-3.1.d displayed no anti-NO effect but did show moderate to effective anticancer activities against A549, MCF-7, HepG2, and WiDr. Notably, condensed tannin ELBT-3.1.d displayed no toxicity to normal cells and a novel potential prebiotic effect on Lactobacillus paracasei BCRC14023 and Lactobacillus rhamnosus BCRC16000. In the animal test, ELCT demonstrated significant effect on reducing plasma glucose in mice at the doses of 50–100 mg/kg BW. The current study contributes to enrich the novel bioactivities of ELCT which may be suggested as functional food or drugs due to processing numerous beneficial bioactivities.

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Acknowledgements

This work was supported in part by a grant from Ton Duc Thang University (No: 3112/2019/QĐ-TĐT), the Ministry of Science and Technology, Taiwan (MOST 106-2320-B-032-001-MY3, MOST109-2923-B-032-001), the Ministry of Education, Taiwan (TKU 0657010), the Ministry of Science and Technology, Taiwan (MOST104-2320-B-077-006-MY3, MOST107-2320-B-077-003-MY3).

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Authors and Affiliations

  1. Division of Computational Mathematics and Engineering, Institute for Computational Science, Ton Duc Thang University, Ho Chi Minh City, 700000, Vietnam

    Van Bon Nguyen

  2. Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, 700000, Vietnam

    Van Bon Nguyen

  3. Faculty of Chemistry, University of Science, Ho Chi Minh City, 700000, Vietnam

    That Quang Ton

  4. Vietnam National University, Ho Chi Minh City, 700000, Vietnam

    That Quang Ton

  5. Department of Science and Technology, Tay Nguyen University, Buon Ma Thuot, 630000, Vietnam

    Dai Nam Nguyen, Thanh Thi Nguyen, Truong Nhan Ngu, Thi Hanh Nguyen, Chien Thang Doan, Thi Ngoc Tran & Minh Trung Nguyen

  6. Institute of Biotechnology and Environment, Tay Nguyen University, Buon Ma Thuot, 630000, Vietnam

    Nhat Duoc Ho & Anh Dzung Nguyen

  7. Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei, 11221, Taiwan

    Yao-Haur Kuo

  8. Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung, 40402, Taiwan

    Yao-Haur Kuo

  9. Department of Chemistry, Tamkang University, New Taipei City, 25137, Taiwan

    San-Lang Wang

  10. Life Science Development Center, Tamkang University, New Taipei City, 25137, Taiwan

    San-Lang Wang

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  1. Van Bon Nguyen
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Contributions

VBN and SLW took part in conceptualization and methodology; VBN involved in software analysis; VBN, SLW, TQT, TNN, ADN, and YHK involved in formal analysis; VBN, DNN, THN, NDH, MTN, CTD, TNT, and TTN involved in investigation; VBN took part in resource collection; VBN took part in data curation; VBN participated in original draft; SLW and VBN involved in review and editing; SLW and VBN took part in supervision; SLW and VBN participated in project administration.

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Correspondence to San-Lang Wang.

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Nguyen, V.B., Ton, T.Q., Nguyen, D.N. et al. Reclamation of beneficial bioactivities of herbal antioxidant condensed tannin extracted from Euonymus laxiflorus. Res Chem Intermed 46, 4751–4766 (2020). https://doi.org/10.1007/s11164-020-04251-3

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  • DOI: https://doi.org/10.1007/s11164-020-04251-3

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