Abstract
A high performance liquid chromatography with ultraviolet detection was proposed for the separation and determination of relative substances in industrial arbutin and its synthetical intermediates 4-acetoxyphenol (ATP) and arbutin pentaacetate (ABPA). Liquid chromatography–mass spectrometry was used for further confirmation of these relative substances. As relative impurities, hydroquione (HQ) and 1,4-diacetoxybenzene (DATB) in industrial ATP, HQ, ATP, and DATB in industrial ABPA, and HQ, ATP, DATB, and ABPA in industrial arbutin were, respectively, quantified. As the results showed, the linearity of standard curves was excellent with relative coefficients of over 0.999 for all the detected components, and the intra- and inter-day precisions of impurities determination were satisfactory, with relative standard deviations of not more than 4.0 %. The distribution and transfer of related substances were elucidated in the synthesis process of arbutin. The established HPLC method not only has great potential in quality control of arbutin but can also be used to instruct the optimization of the synthesis procedure.
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Acknowledgments
This work was supported by National Basic Research Program of China (973 Program, 2009CB421601, 2011CB911003), National Natural Science Foundation of China (21275069, 90913012, 21121091) and Starting Fund for Young Teachers and Analysis & Test Fund of Center of Materials Analysis, Nanjing University.
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Qiao, Jq., Xu, D., Lian, Hz. et al. Analysis of related substances in synthetical arbutin and its intermediates by HPLC–UV and LC–ESI–MS. Res Chem Intermed 41, 691–703 (2015). https://doi.org/10.1007/s11164-013-1221-1
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DOI: https://doi.org/10.1007/s11164-013-1221-1