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New Insights of Turmeric Extract-Loaded PLGA Nanoparticles: Development, Characterization and In Vitro Evaluation of Antioxidant Activity

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Abstract

Here, we presented new insights of the development of poly(lactic-co-glycolic acid) nanoparticles containing turmeric compounds (turmeric-PLGA-NPs) using emulsion-solvent evaporation method. The nanoparticulate system was characterized by size, zeta potential, morphology, release profile, partition parameter, stability and encapsulation efficiency (%EE). Antioxidant activity studies were also evaluated. The Korsmeyer-Peppas model (Mt/M∞ vs. t) was used to determine the release mechanisms of the studied system. Our results demonstrated the emulsion-solvent evaporation method was shown advantageous for producing turmeric-PLGA-NPs in the range of 145 nm with high homogeneity in size distribution, zeta potential of −21.8 mV and %EE about 72%. Nanoparticles were stable over a period of one month. In vitro study showed a release of curcumin governed by diffusion and relaxation of the polymeric matrix. The partition parameter of the extract in relation to blank-PLGA-NPs was 0.111 ± 0.008 M−1, indicating a low affinity of curcumin for the polymer matrix. Antioxidant ability of the turmeric-PLGA-NPs in scavenging the radical 2,2-azinobis (3-ethylbenzothiazoline- 6-sulfonic acid) (ABTS) was inferior to free turmeric extract and showed a concentration and time-dependent profile. The study concluded that PLGA nanoparticles are potential carriers for turmeric extract delivery.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors would like to thank Professor Dr. Pedro Ivo da Silva Maia for technical support and the Multiuser Laboratory of the Chemistry Institute at the Federal University of Uberlândia (UFU) for providing the equipment and technical support for analysis involving scanning electron microscope (SEM).

Funding

This work was supported by National Council of Technological and Scientific Development (CNPq-Brazil) (Grants: 115785/2020–5, 402142/2016–0 and 303659/2019–0), Federal University of Triângulo Mineiro (UFTM) and Research Supporting Foundation of Minas Gerais State (FAPEMIG) (Grant: APQ-02276-18).

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Authors and Affiliations

  1. Department of Nutrition, Federal University of Triângulo Mineiro, Rua Vigário Carlos, Uberaba, Minas Gerais, 38025-350, Brazil

    Camila Maria Gonzales, Kátia Aparecida da Silva & Kélin Schwarz

  2. School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Avenida do Café, s/n,, Ribeirão Preto, São Paulo, 14040-900, Brazil

    Luciana Facco Dalmolin & Renata Fonseca Vianna Lopez

  3. Institute of Exact and Natural Sciences and Education, Federal University of Triângulo Mineiro, Avenida Doutor Randolfo Borges Júnior, Uberaba, Minas Gerais, 38064-200, Brazil

    Natália Bueno Leite Slade & Jeferson Aparecido Moreto

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  1. Camila Maria Gonzales
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Contributions

Kélin Schwarz - Project administration, supervision, formal analysis and write-up; Jéferson A. Moreto - Supervision, formal analysis and write-up; Renata Fonseca Vianna Lopez - Formal analysis; Luciana Facco Dalmolin - Investigation; Camila Maria Gonzales - Investigation; Kátia Aparecida da Silva – Investigation; Natália Bueno Leite Slade – Investigation and write-up.

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Correspondence to Kélin Schwarz.

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Gonzales, C.M., Dalmolin, L.F., da Silva, K.A. et al. New Insights of Turmeric Extract-Loaded PLGA Nanoparticles: Development, Characterization and In Vitro Evaluation of Antioxidant Activity. Plant Foods Hum Nutr 76, 507–515 (2021). https://doi.org/10.1007/s11130-021-00929-0

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