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In Vitro Assay of Quinoa (Chenopodium quinoa Willd.) and Lupin (Lupinus spp.) Extracts on Human Platelet Aggregation

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Abstract

Cardiovascular disease (CVD) is the leading cause of death throughout the world. A major risk factor for CVD is platelet aggregation. Various plant extracts exhibit anti-aggregatory action in vitro. The dietary intake of traditional plant crops such as quinoa (Chenopodium quinoa Willd) and lupin (Lupinus spp., Fabaceae family), highly recognized for their high nutritional value, is increasing worldwide. The aim of the study was to assay possible antiplatelet effects of quinoa and lupin bean extracts in vitro. The proximate chemical composition of quinoa grains and the three most widely known lupin cultivars: blue (L. angustifolius), yellow (L. luteus or mutabilis) and white (L. albus) grown in Chile were analyzed. The anti-aggregation activity of the ethanol extracts of the crops was assayed using flow cytometry in ADP-stimulated human platelets, and their inhibition of the maximal platelet aggregation was measured. All the lupin extracts exhibited a significant anti-aggregatory effect (p < 0.0001), while quinoa extracts did not exert this effect compared to control platelets. In conclusion, lupin beans extracts exhibited an anti-aggregatory effect on activated human platelets.

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Abbreviations

ADP:

Adenosine diphosphate

ANOVA:

Analysis of variance

AOAC:

Association of Official Analytical Chemists

CVD:

Cardiovascular disease

PGE1:

Prostaglandin E1

PPP:

Platelet-poor plasma

PRP:

Platelet-rich plasma

PBS:

Phosphate-buffered saline

TRAP-6:

Thrombin receptor activator peptide

SD:

Standard deviation

SEM:

Standard error of the mean

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Acknowledgements

Project TEKES 1084/31/2016. “Sustainable utilization of Andean and Finnish crops”, University of Turku (Finland), for financial support for the chemical analysis of quinoa grains and lupin beans performed at the Universidad de Valparaíso and Chilean State Universities Network grant 1656-1756.

Author information

Authors and Affiliations

  1. Thematic Task Force on Aging, CUECH Research Network, Santiago, Chile

    Marcelo Alarcón, Eduardo Fuentes, Ivan Palomo & Mariane Lutz

  2. Thrombosis Research Center, Department of Clinical Biochemistry and Immunohematology, Faculty of Health Sciences and Interdisciplinary Excellence Research Program on Healthy Ageing (PIEI-ES), Universidad de Talca, 2 Norte 685, Talca, Chile

    Marcelo Alarcón, Michelle Bustos, Diego Mendez, Eduardo Fuentes & Ivan Palomo

  3. Interdisciplinary Center for Health Studies, CIESAL, Faculty of Medicine, Universidad de Valparaíso, Viña del Mar, Chile

    Mariane Lutz

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  1. Marcelo Alarcón
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Contributions

Conceptualization, Lutz M. and Palomo I.; Methodology, Bustos M.; Mendez D. and Fuentes E. and Writing – Review & Editing, Alarcon M. and Lutz M.

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Correspondence to Ivan Palomo or Mariane Lutz.

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Alarcón, M., Bustos, M., Mendez, D. et al. In Vitro Assay of Quinoa (Chenopodium quinoa Willd.) and Lupin (Lupinus spp.) Extracts on Human Platelet Aggregation. Plant Foods Hum Nutr 75, 215–222 (2020). https://doi.org/10.1007/s11130-019-00786-y

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