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The variability of light-harvesting complexes in aerobic anoxygenic phototrophs

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Abstract

Light-harvesting capacity was investigated in six species of aerobic anoxygenic phototrophic (AAP) bacteria using absorption spectroscopy, fluorescence emission spectroscopy, and pigment analyses. Aerobically grown AAP cells contained approx. 140–1800 photosynthetic reaction centers per cell, an order of magnitude less than purple non-sulfur bacteria grown semiaerobically. Three of the studied AAP species did not contain outer light-harvesting complexes, and the size of their reaction center core complexes (RC–LH1 core complexes) varied between 29 and 36 bacteriochlorophyll molecules. In AAP species containing accessory antennae, the size was frequently reduced, providing between 5 and 60 additional bacteriochlorophyll molecules. In Roseobacter litoralis, it was found that cells grown at a higher light intensity contained more reaction centers per cell, while the size of the light-harvesting complexes was reduced. The presented results document that AAP species have both the reduced number and size of light-harvesting complexes which is consistent with the auxiliary role of phototrophy in this bacterial group.

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Acknowledgments

This research was also supported by GAČR project P501/12/G055 and the EC-funded project Algatech Plus. The authors are indebted to Dr. Hanno Biebl and Prof. Vladimir Gorlenko for their kind gift of AAP strains, Prof. Neil Hunter for providing the Rba. sphaeroides LH2 def. mutant, and Dr. David Bína for recording the excitation spectra of Rsb. litoralis.

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  1. Center Algatech, Institute of Microbiology CAS, 37981, Třeboň, Czech Republic

    Vadim Selyanin, Dzmitry Hauruseu & Michal Koblížek

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  1. Vadim Selyanin
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  2. Dzmitry Hauruseu
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Correspondence to Vadim Selyanin.

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Selyanin, V., Hauruseu, D. & Koblížek, M. The variability of light-harvesting complexes in aerobic anoxygenic phototrophs. Photosynth Res 128, 35–43 (2016). https://doi.org/10.1007/s11120-015-0197-7

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